摘要:
目的：本文对居住于云南省的1020名汉族无关个体19个常染色体短串联重复序列位点进行分析,旨在为法医物证学亲权鉴定和个体识别提供科学全面的群体遗传学基础数据。方法：本研究采用Chelex-100法提取样本DNA、PowerPlex® 21 System试剂盒对D3S1358等19个常染色体的短串联重复序列进行复合扩增。结果：共检出262个等位基因和1006种基因型，经检验，除vWA外，基因型分布均符合Hardy-Weinberg平衡规律(P > 0.05)，累积非父排除率(CPE)为0.99999998，累积个人识别能力(TPD)为9.9 × 10−17。结论：19个常染色体STR基因座在云南汉族人群中具有较高的非父排除率和个体识别能力，可为法医物证学亲权鉴定和个体识别提供基础数据。
Abstract:
Objective: Analyze the loci of 19 autosomal short tandem repeat loci in 1020 unrelated individuals of Han nationality in Yunnan province, China, aiming to provide comprehensive scientific basic data for paternity testing and forensic DNA identification instruments. Methods: In this study, the Chelex-100 method was used to extract sample DNA and the PowerPlex® 21 System kit was used to amplify 19 autosomal short tandem repeats such as D3S1358. Results: A total of 262 alleles and 1006 genotype were detected. After testing, except for vWA, the genotype distribution accords with the Hardy-Weinberg equilibrium (P > 0.05), the cumulative non-parent exclusion rate (CPE) was 0.99999998, and the cumulative personal identification ability (TPD) was 9.9 ×10−17. Conclusion: 19 autosomal str loci have high non-father exclusion rate and individual recognition ability in Yunnan Han population, which can provide good basic data for paternity identification and personal identification of forensic evidence.
文章引用：杨朔, 杨晓佩, 方宝雄, 张寿勋, 计艾岑, 赵涛, 王理琦, 资润, 夏生, 赵斐, 张柠, 张秀峰, 李佳珏, 胡利平, 钟树荣. 云南汉族人群19个常染色体STR基因座遗传多态性研究[J]. 自然科学, 2018, 6(6): 437-443.
https://doi.org/10.12677/OJNS.2018.66057