高同型半胱氨酸通过EMT促进鼻咽癌转移作用及机制

Effect and Mechanism of Hyperhomocysteine on Metastasis of Nasopharyngeal Carcinoma through EMT

摘要: 目的：探讨同型半胱氨酸HCY对鼻咽癌CNE-2细胞侵袭和转移能力的影响及其机制。方法：高浓度HCY处理CNE-2细胞，通过相差显微镜观察细胞形态的改变，通过软琼脂克隆形成以及侵袭和转移实验观察对其克隆形成能力、侵袭和转移能力的影响，并探讨可能的分子机制。结果：HCY处理后，CNE-2细胞从卵圆形向纺锤形转变，伴随着克隆形成能力、侵袭和迁移能力的增强。WB结果显示HCY的处理上调了CNE-2细胞间充质标记物、下调了CNE-2细胞上皮标志物的表达，提示其可能是通过EMT促进CNE-2细胞的恶性转化。

Abstract: Objective: To investigate the effect of Homocysteine on the invasion and migration capability in nasopharyngeal carcinoma CNE-2 cells and to explore the possible mechanism. Methods: After high concentration HCY treatment, the cell morphology of CNE-2 cells was evaluated by phase-contrast microscopy, the colony forming ability was detected by soft agar cloning experiment, and the in-vasion and migration capability was reflexed by invasion and migration experiments. Moreover, the mechanism was also investigated. Results: HCY exposure led to the transformation of the cob-blestone-like cells CNE-2 to spindle-like, fibroblastic cells, accompanied by enhanced colony forming ability and invasion and migration capability. Western blot showed that the mesenchymal marker was up-regulated and the epithelial marker was down-regulated after HCY treatment, which im-plied that HCY might work through EMT to promote the malignant transformation of CNE-2 cells.