西藏粗角猛蚁线粒体基因组序列分析
Mitochondrial Genome Sequence Analysis of Cerapachys xizangensis
DOI: 10.12677/HJAS.2019.92020, PDF,    国家自然科学基金支持
作者: 钟 烨, 周善义, 陈志林*:广西师范大学,珍稀濒危动植物生态与环境保护教育部重点实验室,广西 桂林;广西师范大学,广西珍稀濒危动物生态学重点实验室,广西 桂林
关键词: 蚁科线粒体基因组基因重排引物设计Formicidae Mitochondrial Genome Gene Rearrangement Primer Design
摘要: 报道西藏粗角猛蚁Cerapachys xizangensis基于高通量测序的线粒体基因组全序列,创造性地改进了环状DNA缺口处的引物设计补全序列方法。通过序列分析发现,西藏粗角猛蚁线粒体基因组为闭合的双链DNA分子,总长为16,769 bp,发生了两处基因重排现象:一处为基因洗牌,另一处为基因倒置。
Abstract: This paper reports mitochondrial genome of Cerapachys xizangensis based on high-throughput sequencing, and improves the method of primer design to circular DNA complete sequence creatively. Through the sequence analysis, we found that the mitochondrial genome of Cerapachys xizangensis is a double-stranded DNA molecule, with total length of 16,769 bp, and two gene rearrangements occurred: one is gene shuffle; the other is gene inversion.
文章引用:钟烨, 周善义, 陈志林. 西藏粗角猛蚁线粒体基因组序列分析[J]. 农业科学, 2019, 9(2): 114-125. https://doi.org/10.12677/HJAS.2019.92020

参考文献

[1] Boore, J.L. (1999) Animal Mitochondrial Genomes. Nucleic Acids Research, 27, 1767-1780. [Google Scholar] [CrossRef] [PubMed]
[2] Wang, Y., Chen, J., Jiang, L.Y., et al. (2015) Hemipteran Mitochondrial Genomes: Features Structures and Implications for Phylogeny. International Journal of Molecular Sciences, 16, 12382-12404. [Google Scholar] [CrossRef] [PubMed]
[3] Amaral, D.T., Mitani, Y., Ohmiya, Y., et al. (2016) Organization and Comparative Analysis of the Mitochondrial Genomes of Bioluminescent Elateroidea (Coleoptera: Polyphaga). Gene, 586, 254-262. [Google Scholar] [CrossRef] [PubMed]
[4] Beagley, C.T., Okada, N.A. and Wolstenholme, D.R. (1996) Two Mitochondrial Group I Introns in a Metazoan, the Sea Anemone Metridium senile: One Intron Contains Genes for Subu-nits 1 and 3 of NADH Dehydrogenase. Processdings of the National Academy of Sciences, 93, 5619-5623. [Google Scholar] [CrossRef] [PubMed]
[5] Fukami, H., Chen, C.A., Chiou, C.Y., et al. (2007) Novel Group I Introns Encoding a Putative Homing Endonuclease in the Mitochondrial Cox1 Gene of Scleractinian Corals. Journal of Molecular Evolution, 64, 591-600. [Google Scholar] [CrossRef] [PubMed]
[6] Simon, C., Frati, F., Beckenbach, A., et al. (1994) Evolution, Weighting, and Phylogenetic Utility of Mitochondrial Gene Sequences and a Compilation of Conserved Polymerase Chain Reaction Primers. Annals of the Entomological Society of America, 87, 651-701. [Google Scholar] [CrossRef
[7] Cameron, S.L., Dowton, M., Castro, L.R., et al. (2008) Mitochondrial Genome Organization and Phylogeny of Two Vespid Wasps. Genome, 51, 800-808. [Google Scholar] [CrossRef
[8] Gotzek, D., Clarke, J. and Shoemaker, D. (2010) Mitochondrial Genome Evolution in Fire Ants (Hymenoptera: Formicidae). BMC Evolutionary Biology, 10, 1-13. [Google Scholar] [CrossRef] [PubMed]
[9] Hasegawa, E., Kobayashi, K. and Yagi, N. (2011) Complete Mito-chondrial Genomes of Normal and Cheater Morphs in the Parthenogenetic Ant Pristomyrmex punctatus (Hymenoptera: Formicidae). Myrmecological News, 15, 85-90.
[10] Berman, M., Austin, C.M. and Miller, A.D. (2014) Characterisa-tion of the Complete Mitochondrial Genome and 13 Microsatellite Loci through Next-Generation Sequencing for the New Caledonian Spider-Ant. Leptomyrmexpallens. Molecular Biology Reports, 41, 1179-1187. [Google Scholar] [CrossRef] [PubMed]
[11] de Melo Rodovalho, C., Lyra, M.L. and Ferro, M. (2014) The Mitochondrial Genome of the Leaf-Cutter Ant Atta laevigata: A Mitogenome with a Large Number of Intergenic Spacers. PLoS ONE, 9, 1-9.
[12] Babbucci, M., Basso, A., Scupola, A., et al. (2014) Is It an Ant or a Butterfly? Convergent Evolution in the Mitochondrial Gene Order of Hymenoptera and Lepidoptera. Genome Biology and Evolution, 6, 3326-3343. [Google Scholar] [CrossRef] [PubMed]
[13] Kim, M.J., Hong, E.J. and Kim, I. (2015) Complete Mitochondrial Ge-nome of Camponotus atrox (Hymenoptera: Formicidae): A New tRNA Arrangement in Hymenoptera. Genome, 59, 59-74. [Google Scholar] [CrossRef] [PubMed]
[14] Yang, S., Li, X., Cai, L.G. and Qian, Z.Q. (2016) Characteriza-tion of the Complete Mitochondrial Genome of Formica selysi (Insecta: Hymenoptera: Formicidae: Formicinae). Mito-chondial DNA, 27, 3378-3380. [Google Scholar] [CrossRef] [PubMed]
[15] 唐觉, 李参. 西藏昆虫[M]. 北京: 科学出版社, 1982: 371-374.
[16] Clary, D.O. and Wolstenholme, D.R. (1985) The Mitochondrial DNA Molecule of Drosophila yakuba: Nucleotide Sequence, Gene Organization and Genetic Code. Journal of Molecular Evolution, 22, 252-271. [Google Scholar] [CrossRef
[17] Crozier, R.H. and Crozier, Y.C. (1993) The Mitochondrial Genome of the Honeybee Apis mellifera: Complete Sequence and Genome Organization. Genetics, 133, 97-117.
[18] Szymura, J., Lunt, D. and Hewitt, G. (1996) The Sequence of the Meadow Grasshopper (Chorthippus parallelus) Mitochondrial srRNA, ND2, CO1, CO2, ATPase8 and 9 tRNA Genes. Insect Molecular Biology, 5, 127-139. [Google Scholar] [CrossRef] [PubMed]
[19] Dowton, M., Belshaw, R., Austin, A.D., et al. (2002) Simultaneous Molecular and Morphological Analysis of Braconid Relationships (Insecta: Hymenoptera: Braconidae) In-dicates Independent mt-tRNA Gene Inversions within a Single Wasp Family. Journal of Molecular Evolution, 54, 210-226. [Google Scholar] [CrossRef] [PubMed]
[20] Dowton, M., Stephen, L., Cameron, J., et al. (2009) Characterization of 67 Mitochondrial tRNA Gene Rearrangements in the Hymenoptera Suggests That Mitochondrial tRNA Gene Position Is Selectively Neutral. Molecular Biology and Evolution, 26, 1607-1617. [Google Scholar] [CrossRef] [PubMed]
[21] Tay, W.T., Elfekih, S., Court, L., et al. (2014) Complete Mitochondri-al DNA Genome of Bemisiatabaci Cryptic Pest Species Complex Asia II-5 (Hemiptera: Aleyrodidae). Mitochondial DNA, 27, 1-2.
[22] Nishimoto, M., Okuyama, H., Kiyoshi, T., et al. (2017) Complete Mitochondrial Genome of the Japanese Bumblebee, Bombus Hypocrita Hypocrita (Insecta: Hymenoptera: Apidae). Mitochondrial DNA Part B, 2, 19-20. [Google Scholar] [CrossRef] [PubMed]
[23] Dowton, M. and Austin, A.D. (1999) Evolutionary Dynam-ics of a Mitochondrial Rearrangement “Hot Spot” in the Hymenoptera. Molecular Biology and Evolution, 16, 298-309. [Google Scholar] [CrossRef] [PubMed]
[24] Dowton, M., Castro, L.R., Campbell, S.L., et al. (2003) Frequent Mitochondrial Gene Rearrangement at the Hymenopteran nad3-nad5 Junction. Journal of Molecular Evolution, 56, 517-526. [Google Scholar] [CrossRef] [PubMed]