文冠果花药发育与活性氧平衡的关系
Studies on the Relationship between Anther Development and Reactive Oxygen Balancing in Xanthoceras sorbifolia Bunge
DOI: 10.12677/HJAS.2012.23007, PDF, HTML, XML,  被引量    国家自然科学基金支持
作者: 张淑静, 郑彩霞*, 李凤兰：北京林业大学生物科学与技术学院；王 强：中国科学院水生生物研究所
关键词: 文冠果；花药；雄性不育；活性氧代谢；Xanthoceras sorbifolia B.; Anthers; Male Sterile; Metabolism of Reactive Oxygen
摘要: 本文分析了文冠果不育和可育花药自小孢子母细胞至双核花粉粒发育期间的活性氧的平衡性，结果表明：在发育过程中不育花药中的SOD与CAT活性不协调，Vc含量低，造成H2O2、MDA和有机自由基积累。这可使线粒体等重要细胞器膜破坏，影响了花药发育能量的供应和营养物质的供应而最终导致花药不育。
Abstract: The balancing of the metabolism of reactive oxygen in male fertile anthers and male sterile anthers in Xanthoceras sorbifolia B. from microspore mother cells stage to binuclear stage was studied in this paper. The results indicated that the activity of SOD and CAT was inharmonious, and the content of Vc was less in the male-sterile anthers during their development, which cause the H2O2, MDA and organic free radical accumulating in the male-sterile anthers. As a result, the membranes of some important organelles such as mitochondria were destroyed. It result in the anthers sterile because of impact supply of energy and nutrient substance that anthers upgrowth needed.
文章引用：张淑静, 王强, 郑彩霞, 李凤兰. 文冠果花药发育与活性氧平衡的关系[J]. 农业科学, 2012, 2(3): 37-42. http://dx.doi.org/10.12677/HJAS.2012.23007

参考文献

[1] 王爱国, 植物的氧代谢, 余叔文, 汤章城主编, 植物生理与分子生物学第二版[M]. 北京: 科学出版社, 1998.
[2] R. Mittler. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 2002, 7(9): 405-410.
[3] P. Schopfer, A. Liszkay. Plasma membrane generated reactive oxygen intermediates and their role in cell growth of plants. Bio Factors, 2006, 28: 73-81.
[4] C. Gapper, L. Dolan. Control of plant development by reactive oxygen species. Plant Physiology, 2006, 141: 341-345.
[5] J. Bailey-Serres, R. Mittler. The roles of reactive oxygen species in plant cells. Plant Physiology, 2006, 141: 311-311.
[6] 林植芳, 刘楠. 活性氧调控植物生长发育的研究进展[J]. 植物学报, 2012, 47(1): 74-86.
[7] J. Foreman, V. Demidchik, J. H. F. Bothwell, P. Mylone, H. Miedema, M. A. Torres, P. Linstead, S. Costa, C. Brownlee, J. D. G. Jones, J. M. Davies and L. Dolan. Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. Nature, 2003, 422: 442-446.
[8] M. Sagi, O. Davydov, S. Orazova, Z. Yesbergenova, R. Ophir, J. W. Stratmann, R. Fluhr. Plant respiratory burst oxidase homologes impinge on wound responsiveness and development in Lycopersicon esculentum. Plant Cell, 2004, 16: 616-628.
[9] E. Cadenas. Biochemistry of oxygen toxicity. Ann. Rev. Biochem. , 1989, 58: 79-100.
[10] 王爱国, 邵从本, 罗广华, 郭俊彦, 梁厚果. 活性氧对大豆下胚轴线粒体结构与功能的损伤[J]. 植物生理与分子生物学学报, 1990, (1): 13-18.
[11] 陈少裕. 膜脂过氧化对植物细胞的伤害[J]. 植物生理学通讯, 1991, 27(2): 84-90.
[12] 袁琳, 克热木, 伊力, 张利权. NaCl胁迫对阿月浑子实生苗活性氧代谢与细胞膜稳定性的影响[J]. 植物生态学报, 2005, (6): 119-125.
[13] 刘正鲁, 朱月林, 胡春梅, 魏国平, 杨立飞, 张古文. 氯化钠胁迫对嫁接茄子生长, 抗氧化酶活性和活性氧代谢的影响[J]. 应用生态学报, 2007, (3): 537-541.
[14] 陈贤丰, 梁承邺. HPGMR不育花药能量代谢、H2O2的积累与雄性不育的关系[J]. 植物生理学通讯, 1991, 27(1): 21-24.
[15] 林植芳, 梁承邺, 孙谷畴, 林桂珠. 雄性不育水稻小孢子败育与花药的有机自由基水平[J]. 植物学报, 1993, 35(3): 215- 221.
[16] 张明永, 梁承邺, 段俊, 黄毓文. 油菜细胞质雄性不育系发育进程中活性氧的代谢[J]. 植物学报, 1997, 39(5): 480-482.
[17] 蒋培东, 朱云国, 王晓玲, 朱 伟, 张小全, 解海岩, 王学德. 棉花细胞质雄性不育花药的活性氧代谢[J]. 中国农业科学, 2007, 40(2): 244-249.
[18] 米海莉, 张曦燕, 樊云芳, 李越鲲, 曹有龙. 枸杞雄性不育与植株发育进程中活性氧代谢的关系[J]. 江西农业大学学报, 2008, 30(5): 796-798.
[19] 苗锦山, 杨文才, 李美芹, 沈火林. 葱细胞质雄性不育与花蕾发育过程中活性氧代谢关系研究[J]. 华北农学报, 2009, 24(4): 92-95.
[20] 王俊生. 小麦生理型雄性不育花药活性氧代谢和基因表达分析[D]. 西北农林科技大学, 2010.
[21] 汪李宏. 生理型雄性不育小麦花药结构和活性氧变化的研究[D]. 西北农林科技大学, 2011.
[22] 郑彩霞, 李凤兰. 文冠果两性花花粉败育原因的进一步研究[J]. 北京林业大学学报, 1993, 15(1): 78-84.
[23] 彭伟秀, 王保柱, 李凤兰. 文冠果败育花药和花粉发育的解剖学研究[J]. 河北农业大学学报, 1999, 22(3): 35-37.
[24] 张敏, 王頔, 张雷, 吕雪芹, 唐亮, 王莉. 文冠果雌雄花发育过程形态结构比较[J]. 电子显微学报, 2012, 31(2): 154-162.
[25] 马凯, 高述民, 胡青, 程朋军, 吴江黛, 李凤兰. 文冠果雄蕊发育的解剖学及雄性不育蛋白的研究[J]. 北京林业大学学报, 2004, 26(5): 40-43.
[26] M. M. Bradford. Arapid and sensitive method for the quantification of microgram quanlities of proteins utlizing the principle of protein-dye binding. Anal. Biochem, 1976, 72: 248-254.
[27] 李合生, 孙群, 赵世杰等. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[28] R. S. Dhindsa. Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoide dismutase and catalase. Journal of Exp. Bot, 1981, 32: 93-101.
[29] 赵世杰, 许长城. 植物组织中丙二醛测定方法的改进[J]. 植物生理学通讯, 1994, 30(3): 207-210.
[30] 蔡武城, 袁厚积主编. 生物物质常用化学分析法[M]. 北京: 科学出版社, 1982.
[31] M. Mojović, M. Vuletić, G. G. Bačić and Z. Vučinić. Oxygen radicals produced by plant plasma membranes: An EPR spin- trap study. Journal of Exp Bot, 2004, 55: 2523-2531.
[32] H. J. Rogers. Is there an important role for reactive oxygen species and redox regulation during floral senescence? Plant, Cell and Environment, 2012, 35: 217-233.
[33] 庞勇, 马锋旺, 徐凌飞. 抗坏血酸对苹果组培苗耐热性的生理效应[J]. 果树学报, 2005, 22(2): 160-162.

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