新会柑肉汁产絮培养基的研制及应用

Development and Application of Flocculant-Producing Bacteria Medium Based on the Juice of Xinhui Citrus

摘要: 为了解决微生物絮凝剂大量投产使用所面临的生产成本问题，本研究拟研制新型的廉价产絮培养基。利用新会柑加工废弃果肉的肉汁作为替代碳源(添加量为1%、10%和100%)，制备产絮培养基，经产絮芽孢杆菌发酵后，以高岭土模拟废水评价其絮凝效果，并以奶牛场尾水脱色效果进行验证，以葡萄糖产絮培养基作为对照。结果表明，在新会柑肉汁添加量1%水平下，絮凝效果最好，絮凝率为95.3% (含菌体)和94.7% (不含菌体)，接近对照培养基的絮凝率。对于奶牛场尾水，脱色率达到26.2% (含菌体)和25.9% (不含菌体)，较对照培养基的脱色率提高了一倍以上。可见，新会柑肉汁可用于制备廉价产絮培养基，所产絮凝剂的絮凝效果好，值得推广应用。

Abstract: In order to solve the production cost problem of microbial flocculant, a new type of cheap flocculant-producing bacteria medium was developed. The juice from the waste pulp of Xinhui citrus was taken as an alternative carbon source (adding amounts of 1%, 10% and 100%) to pre-pare the flocculant-producing bacteria medium. After fermentation by Bacillus sp., the flocculation efficiency was evaluated using the kaolin simulated wastewater, and was further verified by the decolorization effect of tail water from the dairy farm. The flocculant-producing bacteria medium based on glucose was used as the control. The results showed that the flocculation efficiency was the best at the adding amounts of 1%, and the flocculation rate was 95.3% (with bacterial cell) and 94.7% (without bacterial cell), which was close to the flocculation rate of the control medium. For the tail water of dairy farm, the decolorization rate reached 26.2% (with bacterial cell) and 25.9% (without bacterial cell), which was more than double that of the control medium. Hence, the juice of Xinhui citrus can be used for the preparation of cheap flocculant-producing bacteria medium, and the produced flocculant has favorable flocculation effect, which is worthy of a wide application.