常用杀虫剂辛硫磷、阿维毒死稗的光谱特征研究

Spectroscopic Studies on the Aqueous Solution of Commonly Used Insecticide Phoxim and Avermectin Chlorpyrifos

摘要: 利用紫外–可见吸收光谱、荧光光谱和三维荧光光谱等技术研究了杀虫剂(辛硫磷、阿维•毒死蜱)水溶液的光谱特性。结果表明，在相同的实验条件下，辛硫磷在275 nm处有特征吸收峰，阿维•毒死蜱在335 nm处有特征吸收峰；辛硫磷和阿维•毒死蜱水溶液的荧光光谱、三维荧光光谱具有显著的差异，辛硫磷有两个荧光区域，在λ_ex\λ_em = 245 nm~285 nm\270 nm~310 nm处，激发波长为265 nm，峰值位置为292 nm；在λ_ex\λ_em = 245 nm~300 nm\310 nm~380 nm处，最佳激发波长为275 nm，有两处荧光峰，峰值位置分别为322 nm和334 nm；阿维•毒死蜱的主要荧光区域在λ_ex\λ_em = 310 nm~370 nm\360 nm~510 nm处，共有两处峰，峰值位置分别在422 nm和424 nm处，所对应的激发波长为335 nm和355 nm。本研究为农药杀虫剂残留的定性检测分析提供了实验参考。

Abstract: Fluorescence spectra and fluorescence spectra of three-dimensional technology is utilized to study the insecticide (phoxim, avermectin chlorpyrifos) spectral characteristics of an aqueous solution of the absorption spectra. The result shows that the phoxim characteristic absorption peak is at 275 nm and the avermectin chlorpyrifos characteristic absorption peak is at 335 nm under the same experimental conditions. Fluorescence spectroscopy and phoxim, avermectin chlorpyrifos aqueous solution of three-dimensional fluorescence spectrum have significant differences. There are two fluorescent areas of phoxim in λex\λem = 245 nm - 285 nm\270 nm - 310 nm, the excitation wave-length is 265 nm and the peak position is 292 nm. In λex\λem = 245 nm - 300 nm\310 nm - 380 nm and the best excitation wavelength is at 275 nm, there are two fluorescence peaks, the peak positions are 322 nm and 334 nm; Fluorescent area distribution of avermectin chlorpyrifos is mainly in λex\λem = 310 nm - 370 nm\360 nm - 510 nm. Both of two peaks positions are at 422 nm and 424 nm, respectively, the corresponding excitation wavelength is 335 nm and 355 nm. This study analyzed the qualitative detection of insecticide to provide a reference for the experiment.