应用低场核磁共振技术测定D-果糖溶液浓度的方法研究
Detection of D-Fructose Solution with Different Concentrations by Low Field NMR
DOI: 10.12677/MP.2019.96030, PDF,    科研立项经费支持
作者: 张 凤, 钟 鹏*, 李诗妍：中国石油大学(华东)理学院，山东 青岛
关键词: D-果糖；浓度；低场核磁共振；CPMG序列；横向弛豫时间；峰面积；D-Fructose； Concentration； Low Field NMR； CPMG Sequence； Transverse Relaxation Time； Peak Area
摘要: 为了探索利用低场核磁共振技术测量D-果糖水溶液浓度的方法，本文采取CPMG自旋回波法，分析不同浓度D-果糖水溶液T2谱特征，找出各峰横向弛豫时间、峰面积与浓度之间的相关性。实验结果表明：随着D-果糖水溶液浓度增大，对应的“自由水”峰横向弛豫时间减小，“束缚水”峰共振峰面积增大，变化均呈一次线性相关，且拟合方程的R2均大于0.98，拟合精度较高。在此基础上提出了两种D-果糖水溶液浓度的核磁共振测量方法，并且通过实例验证，表明该方法可靠有效。
Abstract: In order to explore the method of measuring the concentration of d-fructose aqueous solution by low field nuclear magnetic resonance (NMR) technique, CPMG spin echo method was adopted in this paper to analyze the T2 spectrum characteristics of d-fructose aqueous solution with different concentrations, and to find out the correlation between the transverse relaxation time, peak area and concentration. The experiment results showed that as the concentration of d-fructose in aqueous solution increased, the transverse relaxation time of the “free water” decreased, and the formant area of the “bound water” increased, both of which showed linear change, and the R2 of the fitting equations were all greater than 0.98, indicating a high fitting accuracy. On this basis, two NMR methods for measuring the concentration of d-fructose aqueous solution were proposed, and the results showed that the method was reliable and effective.
文章引用：张凤, 钟鹏, 李诗妍. 应用低场核磁共振技术测定D-果糖溶液浓度的方法研究[J]. 现代物理, 2019, 9(6): 324-331. https://doi.org/10.12677/MP.2019.96030

参考文献

[1] 黄婷, 钟浪声, 倪海明, 等. D-果糖的性质、应用及市场前景[J]. 大众科技, 2017, 19(3): 40-42.
[2] Dounia, E.H., Henri, G., Jean Louis, G., et al. (2018) Consumption of Alcopops during Brain Maturation Period: Higher Impact of Fructose than Ethanol on Brain Metabolism. Frontiers in Nutrition, 5, 33.
[Google Scholar] [CrossRef] [PubMed]
[3] 罗雅君, 李珂, 赵琳, 等. 果糖的利用现状及其与现代流行病的关系研究进展[J]. 农产品加工·学刊(下), 2013(20): 60-63.
[4] 杨泉女, 周权驹, 王蕴波, 等. 甜玉米葡萄糖、果糖和蔗糖含量近红外反射光谱模型构建[J]. 中国农业科技导报, 2018, 20(1): 137-146.
[5] 岳虹, 赵贞, 刘丽君, 等. 高效液相色谱法测定发酵乳饮料中果糖、葡萄糖、蔗糖、麦芽糖及乳糖含量[J]. 乳业科学与技术, 2017, 40(2): 23-26.
[6] Rajkumar, R., Warsinke, A., Mohwald, H., et al. (2008) Analysis of Recognition of Fructose by Imprinted Polymers. Talanta, 76, 1119-1123.
[Google Scholar] [CrossRef] [PubMed]
[7] Bhand, S. (2010) Fructose-Selective Calorimetric Biosensor in Flow Injection Analysis. Analytica Chimica Acta, 668, 13-18.
[Google Scholar] [CrossRef] [PubMed]
[8] Ruizmatute, A.I., Rodriguezsanchez, S., Sanz, M.L., et al. (2010) Detection of Adulterations of Honey with High Fructose Syrups from Inulin by GC Analysis. Journal of Food Composition & Analysis, 23, 273-276.
[Google Scholar] [CrossRef] 
[9] 吕敏红. 新型长波长荧光探针的合成及性质研究[D]: [硕士学位论文]. 西安: 西北大学, 2014.
[10] Erikson, U., Standal, I.B., Aursand, I.G., et al. (2012) Use of NMR in Fish Processing Optimization: A Review of Recent Progress. Magnetic Resonance in Chemistry, 50, 471-480.
[Google Scholar] [CrossRef] [PubMed]
[11] Liao, S.H. and Wu, P.C. (2017) A Study of Spin-Lattice Relaxation Rates of Glucose, Fructose, Sucrose and Cherries Using High-Tc SQUID-Based NMR in Ultralow Magnetic Fields. Superconductor Science & Technology, 30, Article ID: 084006.
[Google Scholar] [CrossRef] 
[12] 刘纯友, 马美湖, 王庆玲, 等. 核磁共振技术在食品脂质研究中的应用新进展[J]. 食品工业科技, 2017, 38(12): 342-346.
[13] 朱俊, 郭原, 尚鹤龄, 等. 核磁共振实验的误差研究[J]. 云南师范大学学报: 自然科学版, 2009, 29(3): 43-45.
[14] 白怀勇, 周格, 王殿生. 不同浓度乙醇-水溶液核磁共振实验研究[J]. 实验技术与管理, 2013(8): 39-42.
[15] 高铁军, 孟祥省, 王书运. 近代物理实验[M]. 北京: 科学出版社, 2009.
[16] 邢庆洋, 庞明利. 结晶果糖在饮料领域的应用优势[J]. 食品安全导刊, 2016(7): 50-51.
[17] 熊婷, 张英力, 蔡清. 基于低场磁共振技术的糖溶液检测[J]. 中国计量大学学报, 2013, 24(3): 219-224.
[18] Van, D. (2010) Applications of Time-Domain NMR in Food Science and Technology. London Royal Society of Chemistry, 36, 50-53.
[19] Ruan, R.R., Wang, X., Chen, P.L., et al. (1999) Study of Water in Dough Using Nuclear Magnetic Resonance. Cereal Chemistry, 76, 231-235.
[Google Scholar] [CrossRef] 
[20] 俎栋林. 核磁共振成像学[M]. 北京: 高等教育出版社, 2004: 46-53.
[21] 李资玲, 刘成梅, 万婕, 等. 核磁共振研究膳食纤维面包制作过程的水分迁移行为[J]. 食品科学, 2007, 28(10): 127-130.