一种基于甲酰基香豆素识别Cys/Hcy的荧光性能研究
Study on the Fluorescence Properties of Cys/Hcy Based on Formyl Coumarin
DOI: 10.12677/HJCET.2020.102008, PDF,  被引量   
作者: 张汉涛, 胡路平, 夏胜涛, 李洪启*, 咸春颖, 夏婷婷, 张洪雨, 李 颢, 杨 琳：东华大学化学化工与生物工程学院，上海
关键词: 甲酰基；香豆素；荧光探针；Cys/Hcy；Formyl； Coumarin； Fluorescent Probe； Cys/Hcy
摘要: 设计、合成了一种基于甲酰基识别L-半胱氨酸(Cys)和L-高半胱氨酸(Hcy)的香豆素荧光探针。该探针在HEPES缓冲液(EtOH:H2O = 8:2 (v:v), pH = 7.1)中，对Cys和Hcy具有良好的选择性和较高的灵敏度。探针耐酸碱，在2~10的广泛pH区域内，对Cys和Hcy仍表现出良好的识别效果。在探针溶液中加入等当量的Cys或Hcy后，体系荧光强度增强2~3倍，响应时间分别为30 min、20 min。10当量其它常见氨基酸对其检测无干扰，对Cys和Hcy的最低检测限分别达到5.7 × 10−7 mol/L和5.2 × 10−7 mol/L。
Abstract: A coumarin fluorescent probe based on formyl recogniting L-cysteine (Cys) and L-homocysteine (Hcy) was designed and synthesized. The probe has good selectivity and high sensitivity to Cys and Hcy in HEPES buffer (EtOH:H2O = 8:2 (v:v), pH = 7.1). The probe is resistant to acids and al-kalis, and still shows good recogniting effect on Cys and Hcy in a wide pH range of 2~10. After adding equivalent Cys or Hcy to the probe solution, the fluorescence intensity of the system en-hances 2~3 times, responsing time is 30 min, 20 min. 10 equivalents of other common amino acids have no interference on the detection. The lowest detection limits are 5.7 × 10−7 mol/L and 5.2 × 10−7 mol/L.
文章引用：张汉涛, 胡路平, 夏胜涛, 李洪启, 咸春颖, 夏婷婷, 张洪雨, 李颢, 杨琳. 一种基于甲酰基香豆素识别Cys/Hcy的荧光性能研究[J]. 化学工程与技术, 2020, 10(2): 48-60. https://doi.org/10.12677/HJCET.2020.102008

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