AQP1促进鼻咽癌细胞的迁移和侵袭
Aquaporin 1 Promotes the Migration and Invasion of Nasopharyngeal Carcinoma Cell in Vitro
DOI: 10.12677/ACM.2020.104102, PDF,    科研立项经费支持
作者: 覃云英, 罗宁斌：广西医科大学附属肿瘤医院，广西 南宁
关键词: 水通道蛋白1；鼻咽癌；迁移；侵袭；Aquaporin 1； Nasopharyngeal Carcinoma； Migration； Invasion
摘要: 目的：探讨水通道蛋白1 (AQP1)对人鼻咽癌细胞迁移及侵袭能力的影响。方法：采用shRNA转染人鼻咽癌细胞5-8F后，用Western blot检测AQP1蛋白的表达量，Transwell实验检测细胞的迁移及侵袭能力。结果：shRNA质粒转染细胞能显著降低人鼻咽癌细胞5-8F中AQP1的蛋白表达水平(P < 0.05)。Transwell实验发现抑制AQP1的表达可降低鼻咽癌细胞的迁移侵袭能力(P < 0.05)。结论：AQP1可促进鼻咽癌细胞的迁移和侵袭，可成为鼻咽癌治疗后潜在靶点。
Abstract: Objective: To investigate the effects of AQP1 on nasopharyngeal carcinoma cell migration and invasion. Methods: Nasopharyngeal carcinoma cell 5-8F was transfected with short hairpin RNA (shRNA) targeting AQP1. And then, Western blot was used to detect the AQP1 protein expression, and Transwell assay was used to detect cell migration and invasion. Results: The expression of AQP1 was effectively downregulated in nasopharyngeal carcinoma cell by shRNA targeting AQP1. Knockdown of AQP1 inhibited nasopharyngeal carcinoma cell migration and invasion in vitro (P < 0.01). Conclusion: Aquaporin 1 can promote the migration and invasionin nasopharyngeal carcinoma cell. Thus, AQP1 may serve as a potential therapeutic target for the treatment of TNBC.
文章引用：覃云英, 罗宁斌. AQP1促进鼻咽癌细胞的迁移和侵袭[J]. 临床医学进展, 2020, 10(4): 652-657. https://doi.org/10.12677/ACM.2020.104102

参考文献

[1] 曹骥, 李秋林, 容敏华, 葛莲英, 余家华. 2015年广西肿瘤登记地区恶性肿瘤发病和死亡分析[J]. 中国癌症防治杂志, 2019, 11(1): 43-51.
[2] 中国抗癌协会鼻咽癌专业委员会, 陈晓钟, 李金高, 等. 转移性鼻咽癌治疗专家共识[J]. 中华放射肿瘤学杂志, 2018, 27(1): 23-28.
[3] 覃云英, 罗宁斌, 王鹏, 等. AQP1在不同转移潜能的人鼻咽癌细胞株中的表达[J]. 临床医学进展, 2018, 8(10): 948-952.
[4] 李卓, 康炜, 辛娜, 等. AQP1-shRN A 表达质粒载体的构建与筛选[J]. 重庆医学, 2015(30): 4183-4186.
[5] 张珺, 于世英, 殷铁军. AQP1、HPA和VEGF在非小细胞肺癌组织中的表达和临床意义[J]. 中国现代医学杂志, 2009, 19(18): 2750-2753+2756.
[6] 张斌, 刘芳芳, 马勇杰, 等. 水通道蛋白1在乳腺癌细胞质中的表达及其与患者临床病理特征和预后的关系[J]. 中华肿瘤杂志, 2013, 35(12): 904-909.
[7] Kang, B.W., Kim, J.G., Lee, S.J., et al. (2015) Expression of Aquaporin-1, Aqua-porin-3, and Aquaporin-5 Correlates with Nodal Metastasis in Colon Cancer. Oncology, 88, 369-376. [Google Scholar] [CrossRef] [PubMed]
[8] Chetry, M., Li, S., Liu, H., et al. (2018) Prognostic Values of Aquaporins mRNA Expression in Human Ovarian Cancer. Bioscience Reports, 38. [Google Scholar] [CrossRef] 
[9] 李琦, 张威, 张宝. 鼻咽癌中水通道蛋白1表达与血管生成能力的相关性研究[J]. 临床耳鼻咽喉头颈外科杂志, 2010, 24(24): 1115-1118.
[10] Steeg, P.S. (2006) Tumor Metastasis: Mechanistic Insights and Clinical Challenges. Nature Medicine, 12, 895-904. [Google Scholar] [CrossRef] [PubMed]
[11] Nguyen, D.X., Bos, P.D. and Massagué, J. (2009) Metastasis: From Dis-semination to Organ-Specific Colonization. Nature Reviews Cancer, 9, 274-284. [Google Scholar] [CrossRef] [PubMed]
[12] Wei, X. and Dong, J. (2015) Aquaporin 1 Promotes the Proliferation and Migration of Lung Cancer Cell in Vitro. Oncology Reports, 34, 1440-1448. [Google Scholar] [CrossRef] [PubMed]
[13] Wang, Y., Fan, Y., Zheng, C., et al. (2017) Knockdown of AQP1 In-hibits Growth and Invasion of Human Ovarian Cancer Cells. Molecular Medicine Reports, 16, 5499-5504. [Google Scholar] [CrossRef] [PubMed]
[14] Verkman, A.S. (2002) Aquaporin Water Channels and Endothelial Cell Function. Journal of Anatomy, 200, 617-627. [Google Scholar] [CrossRef] [PubMed]
[15] Verkman, A.S., Hara-Chikuma, M. and Papadopoulos, M.C. (2008) Aquaporins-New Players in Cancer Biology. Journal of Molecular Medicine, 86, 523-529. [Google Scholar] [CrossRef] [PubMed]
[16] Verkman, A.S. (2009) Knock-out Models Reveal New Aquaporin Functions. Handbook of Experimental Pharmacology, 190, 359-381. [Google Scholar] [CrossRef] [PubMed]

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