产志贺毒素大肠杆菌基因分型技术的研究进展
Research Progress in Genotyping of Shiga Toxin-Producing Escherichia coli
DOI: 10.12677/AMB.2020.93018, PDF,    科研立项经费支持
作者: 张世钦, 王梓晨, 白芷烨, 李红梅*, 王 翔, 董庆利：上海理工大学医疗器械与食品学院，上海；汪 雯, 吉小凤：农产品质量安全危害因子与风险防控国家重点实验室(筹)，浙江省农业科学院农产品质量标准研究所，农业农村部农产品质量安全风险评估实验室(杭州)，浙江 杭州
关键词: 产志贺毒素的大肠杆菌；分型技术；全基因组测序；Shiga Toxin-Producing Escherichia coli； Genotyping Methods； Whole Genome Sequencing
摘要: 产志贺毒素大肠杆菌(Shiga toxin-producing Escherichia coli, STEC)是重要的食源性致病菌，能引起人和动物发生腹泻、出血性肠炎和溶血性尿毒综合症等疾病。为了更好的开展STEC检测、表征、分子进化分析及溯源调查，本文对国内外STEC基因分型技术包括基于酶切扩增分型方法、基于全基因组测序(Whole genome sequencing, WGS)分型方法以及多位点串联重复序列分析(MLVA)、DNA微阵列(DNA microarray)和成簇规律间隔短回文重复序列(CRISPR)分型技术研究进展进行综述，并对基因分型研究方法存在的问题进行探讨，为STEC的溯源追踪和流行病学调查提供参考。
Abstract: Shiga Toxin-producing Escherichia coli (STEC) is an important food-borne pathogen, which can cause diseases, such as diarrhea, hemorrhagic enteritis and hemolytic uremic syndrome in humans and animals. In order to implement the detection, characterization, molecular evolution analysis and traceability investigation of STEC more efficiently, the worldwide STEC genotyping techniques including the genotyping methods based on enzyme-cut amplification and Whole Genome Sequencing (WGS), Multiple Locus Variable number of tandem repeat Analysis (MLVA), DNA microarray and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were reviewed, and the issues of genotyping methods were discussed, which could provide the valuable information for the STEC traceability and epidemiological investigation.
文章引用：张世钦, 汪雯, 吉小凤, 王梓晨, 白芷烨, 李红梅, 王翔, 董庆利. 产志贺毒素大肠杆菌基因分型技术的研究进展[J]. 微生物前沿, 2020, 9(3): 125-132. https://doi.org/10.12677/AMB.2020.93018

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