高产谷胱甘肽酵母的诱变筛选及发酵条件的优化

Mutagenesis Screening of High-Yielding Glutathione Yeast and Optimization of Fermentation Conditions

摘要: 为了更好的利用微生物发酵合成谷胱甘肽(glutathione, GSH)，本研究通过对产朊假丝酵母菌株进行紫外诱变，并选取抗性筛选物乙硫氨酸对出发菌株进行致死率实验，筛选到一株高产谷胱甘肽突变菌株。采用5因素5水平的正交设计实验对发酵合成GSH的培养条件中培养温度、摇床转速、蛋白胨和硫酸铵浓度之比、初始pH值及接种量的组成进行优化。结果表明：发酵条件为培养温度26℃、200 r/min、蛋白胨和硫酸铵浓度之比5:2、初始pH值5.0及接种量10%时突变菌株的谷胱甘肽产量最高，达到了187.31 mg/L，即有利于菌体合成GSH。

Abstract: In order to synthesize glutathione (GSH) by microbial fermentation, a mutant strain with high glutathione yield was screened in this study through UV mutagenesis of Candida utilis and lethality test on the original strain by selecting ethionine as resistant screening material. The orthogonal design experiment with 5 factors and 5 levels was used to optimize the fermentation conditions of glutathione synthesis, including the culture temperature, the rotation speed of the shaking table, the ratio of peptone and ammonium sulfate concentration, the initial pH value and the composition of inoculation amount. The results showed that when the fermentation temperature was 26˚C, 200 r/min, the ratio of tone and ammonium sulfate was 5:2, the initial pH value was 5.0 and the inoculation amount was 10%, the glutathione yield of the mutant strain was the highest, which reached 187.31 mg/L, which was conducive to the synthesis of GSH.