一株降解呕吐毒素细菌的筛选及其鉴定
Screening and Identification of a Deoxynivalenol (DON)-Degrading Bacteria
DOI: 10.12677/AMB.2021.101006, PDF,   
作者: 范卓莹, 余 挺*：浙江大学生物系统工程与食品科学学院，馥莉食品科学研究院，农业部农产品营养评价重点实验室，农业部农产品采后处理重点实验室，智能食品加工技术与装备国家地方联合工程实验室，浙江 杭州
关键词: 呕吐毒素；降解；筛选鉴定；解单端孢菌素微杆菌；Deoxynivalenol (DON)； Degradation； Screening and Identification； Microbacterium trichothecenolyticum
摘要: 本文利用苯基环氧乙烷为唯一碳源从咖啡渣中筛选出7株具有呕吐毒素降解潜力的菌株，通过iElisa酶联免疫法确定呕吐毒素降解率，最终得到一株呕吐毒素降解能力较强的菌株XY-3。通过分子生物学技术对其进行鉴定，并初步探究了其生理生化特性。结果表明，筛选得到的菌株XY-3为解单端孢菌素微杆菌(Microbacterium trichothecenolyticum)，其在24 h内对呕吐毒素的降解率可达到27.71%。XY-3为革兰氏阳性菌，可利用多种碳源，并具有较强的耐盐性，但是对酸性环境较敏感。本研究可为呕吐毒素生物降解拓展研究范围，并为后续的研究提供基础支撑。
Abstract: In this paper, 7 strains with deoxynivalenol (DON) degradation potential were selected from coffee grounds using styrene oxide as the only carbon source, and the degradation rate of DON was determined. Finally, a strain XY-3 with the outstanding degrading ability of DON was obtained, which was identified by molecular biology technology, and its physiological and biochemical characteristics were preliminarily explored. The results showed that XY-3 was identified as Microbacterium trichothecenolyticum, and its degradation rate of DON can reach 27.71% within 24 hours. XY-3 is a gram-positive bacterium that can use a variety of carbon sources and has strong salt tolerance, but it is more sensitive to acidic environments. This study can expand the scope of research for the biodegradation of DON and provide basic support for further research.
文章引用：范卓莹, 余挺. 一株降解呕吐毒素细菌的筛选及其鉴定[J]. 微生物前沿, 2021, 10(1): 43-51. https://doi.org/10.12677/AMB.2021.101006

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