生物医学  >> Vol. 2 No. 1 (January 2012)

基于CdTe和CdSe量子点体外PDT灭活白血病HL60细胞实验研究
Experimental Research on the Vitro Inactivation of HL60 Cells Based on CdTe and CdSe Quantum Dots

DOI: 10.12677/hjbm.2012.21001, PDF, HTML, 下载: 2,836  浏览: 10,296  国家自然科学基金支持

作者: 贺宇路*, 黄康强, 姜飞, 王健, 熊建文

关键词: CdSeCdTe量子点(QDs)光动力疗法(PDT)CCK-8<
CdSe; CdTe; Quantum Dots (QDs); Photodynamic Therapy (PDT); CCK-8

摘要: 近年来,随着光动力疗法基础研究的不断深入,量子点作为潜在的光敏剂对癌细胞的灭活效应引起了人们的极大关注,实验利用羧基乙酸包覆的量子点CdTe和CdSe进行了QDs-PDT体外灭活HL60细胞的实验研究。实验结果表明:CdTe和CdSe的对HL60细胞灭活的最佳光照剂量分别为15 J/cm2和18 J/cm2,最佳作用浓度均为2.0 µmol/L;在最佳作用参数下两者的灭活效率分别为62.7%,83.0%,基于CdSe的QDs-PDT灭活效率优于CdTe。
Abstract: Quantum dots used to destruct the cancer cells through the photocatalytic reaction has caused great attention in recent years. In the paper, thiol-capped CdTe and CdSe QDs were used to destruct the HL60 cells. The results showed that the best light irradiation dose of CdTe and CdSe were 15 J/cm2 and 18 J/cm2. The best concentration of additive QDs were all 2.0 µmol/L. The inactivation efficiency were 62.7% and 83.0% under the best destruction para- meters, respectively. Additionally, the destruction efficiency of CdSe is better than that of CdTe in the optimized condi- tions.

文章引用: 贺宇路, 黄康强, 姜飞, 王健, 熊建文. 基于CdTe和CdSe量子点体外PDT灭活白血病 HL60细胞实验研究 [J]. 生物医学, 2012, 2(1): 1-5. http://dx.doi.org/10.12677/hjbm.2012.21001

参考文献

[1] J. Tyrrell, S. M. Campbell and A. Curnow. Monitoring the accu- mulation and dissipation of the photosensitizer protoporphyrin IX during standard dermatological methyl-aminolevulinate photo- dynamic therapy utilizing noninvasive fluorescence imaging and quantification. Photodiagnosis and Photodynamic Therapy, 2011, 1(8): 30-38.
[2] C. P. Chang, D. J. Nagel and M. E. Zaghloul. Irradiance depen- dence of photobleaching of resorufin. Journal of Photochemistry and Photobiology A: Chemistry, 2011, 2(217): 430-432.
[3] J. S. Dysart, M. S. Patterson, T. J. Farrell, et a1. Relationship between mTHPC fluorescence photobleaching and cell viability during in Vitro photodynamic treatment of DP16 cells. Photo- chemistry Photobiology A, 2002, 2(75): 289-295.
[4] R. Bakalova, H. Ohba, Z. Zhelev, et al. Quantum dot anti2CD conjugates: Are they potential photosensitizers or potentiators of classical photosensitizing agents in photody namictherapy of cancer. Nano Letters, 2004, 9(4): 1567-1573.
[5] A. C. S. Samia, X. B. Chen and C. Burda. Semiconductor quan- tum dots for photodynamic therapy. Journal of the American Che- mical Society, 2003, 51(125): 15736-15737.
[6] R. Generalov, S. Kavaliauskiene1, S. Westrom, et al. Entrapment in phospholipid vesicles quenches photoactivity of quantum dots. International Journal of Nanomedicine, 2011, 6: 1875-1888.
[7] S. P. Singh. Multifunctional magnetic quantum dots for cancer theranostics. Journal of Biomedical Nanotechnology, 2011, 7(1): 95-97.
[8] M. Nyk, K. Palewska, L. Kepinski, K. A. Wilk, et al. Fluore- scence resonance energy transfer in a non-conjugated system of CdSe quantum dots/zinc-phthalocyanine. Journal of Lumine- scence, 2010, 12(130): 2487-2490.
[9] C. R. Kagan, C. B. Murray and G. Bawendim. Long-range resonance transfer of electronic excitations in close-packed Cdse quantum dot solids. Physical Review, 1996, 12(54): 8633-8643.
[10] L. Jasmina, J. C. Sung, M. Francoise, et al. Unmodified cad- mium telluride quantum dots induce resctive oxygen species formation leading to multiple organelle damage and cell death. Chemistry and Biology, 2005, 11(12): 1227-1234.
[11] V. Biju, S. Mundayoor, R. V. Omkumar, et al. Bioconjugated quantum dots for cancer research: Present status, prospects and remaining issues. Biotechnology, 2010, 2(28): 199-213.
[12] H. Zeng, M. Korbelik, D. I. Mclean, et a1. Monitoring photo- product formation and photobleaching by fuorescence spectro- scopy has the potentialto improve PDT dosimetry with a verte- porfin-like photosensitizer. Photochem Photobiol, 2002, 4(75): 398-405.
[13] 熊建文, 肖化, 张镇西. MTT法和CCK-8法检测细胞活性之测试条件比较[J]. 激光生物学报, 2007, 5(16): 526-531.
[14] 肖化, 熊建文, 吴继明等. ALA-PDT对白血病肿瘤细胞作用的参数研究[J]. 激光生物学报, 2004, 5(13): 353-357.
[15] D. D. Xu, W. S. C. Cho, P. Wu, et al. Photo-activated pheo- phorbide a inhibits the growth of prostate cancer cells. Laser Physics, 2011, 9(21): 1670-1674.
[16] Y. Han, G. Y. Xie, N. Liu, et al. Effects of CdTe quantum dots on the antioxidant enzymes activity and lipid peroxidation in testes of mice. Asian Journal of Ecotoxicology, 2010, 6(5): 894-898.