生物炭对农田土壤中氮循环及氧化亚氮产生的影响与机理
Effect and Mechanism of Biochar on Nitrogen Cycle and Nitrous Oxide Production in Farmland Soil
DOI: 10.12677/OJNS.2023.112029, PDF,   
作者: 范红叶, 叶孝杰, 吴文豪:浙江树人学院生物与环境工程学院,浙江 杭州;王泽宇*:浙江树人学院交叉科学研究院,浙江 杭州
关键词: N2O氮循环硝态氮铵态氮生物炭N2O Nitrogen Cycle Nitrate Nitrogen Ammonium Nitrogen Biochar
摘要: 氧化亚氮(N2O)作为温室气体和臭氧层破坏者备受学者关注,其中农田土壤是非人为条件下N2O最主要的排放源。土壤中参与氮素循环的生物/非生物过程中复杂多变,探索不同路径的氮转化机制以及对N2O的贡献度有助于对N2O减排提供机理剖析。生物炭因其高孔隙度、强吸附性、化学稳定性和大阳离子交换量等优点,会对土壤中氮素的转化产生直接/间接的影响,并显著改善/恶化土壤N2O排放。因此,总结了生物炭对土壤生态系统中氮素的转化与N2O排放的研究现状,分别论述了生物炭对无机氮循环与N2O排放的影响,并从生物炭吸附、影响土壤理化性质、群落结构多样性以及关键功能基因等方面揭示了其作用机制。基于以上内容,对今后生物炭在N2O增汇减排领域的进一步理论研究和相关技术推广进行了展望。
Abstract: Nitrous oxide (N2O) as a greenhouse gas and ozone layer destroyer has attracted much attention from scholars, and farmland soil is the main source of N2O emissions under non-human conditions. The biotic/abiotic processes involved in the nitrogen cycle in soil are complex and changeable. Exploring the nitrogen transformation mechanism of different pathways and the contribution to N2O will help provide a mechanism analysis for N2O emission reduction. Due to its advantages of high porosity, strong adsorption, chemical stability and large cation exchange capacity, biochar can have direct/indirect effects on nitrogen transformation in soil and significantly improve/worse soil N2O emissions. Therefore, this paper summarizes the research status of biochar on nitrogen transformation and N2O emission in soil ecosystems, discusses the effects of biochar on inorganic nitrogen cycle and N2O emission, and discusses the effects of biochar adsorption, soil physical and chemical properties, and community structure. Diversity as well as key functional genes revealed its mechanism of action. Based on the above content, the further theoretical research and related technology promotion of biochar in the field of N2O sink emission reduction in the future are prospected.
文章引用:范红叶, 叶孝杰, 吴文豪, 王泽宇. 生物炭对农田土壤中氮循环及氧化亚氮产生的影响与机理[J]. 自然科学, 2023, 11(2): 243-252. https://doi.org/10.12677/OJNS.2023.112029

参考文献

[1] Liu, C.-R., Zhu, J.-Y., Li, Y.-Y., et al. (2022) Emission of Nitrous Oxide (N2O) from Lake Taihu and the Corre-sponding Potential Driving Factors. Environmental Science, 43, 4118-4126.
[2] Huo, P., Liu, Y., Xu, C., et al. (2023) Characteristics of Dissolved N2O and Indirect N2O Emission Factor in the Groundwater of High Nitrate Leaching Areas in Northwest China. Science of the Total Environment, 868, Article ID: 161646. [Google Scholar] [CrossRef] [PubMed]
[3] Peng, X., Ji, Q., Angell, J.H., et al. (2021) Long-Term Fertilization Alters Nitrous Oxide Cycling Dynamics in Salt Marsh Sediments. Environmental Science & Technology, 55, 10832-10842. [Google Scholar] [CrossRef] [PubMed]
[4] Asadyar, L., Xu, C.-Y., Wallace, H.M., et al. (2021) Soil-Plant Nitrogen Isotope Composition and Nitrogen Cycling after Biochar Applications. Environmental Science and Pollution Research, 28, 6684-6690. [Google Scholar] [CrossRef] [PubMed]
[5] Xu, H., Wang, C., Liang, Z., et al. (2015) The Structure and Component Characteristics of Partial Nitrification Biofilms under Autotrophic and Heterotrophic Conditions. Ap-plied Microbiology and Biotechnology, 99, 3673-3683. [Google Scholar] [CrossRef] [PubMed]
[6] Zhang, N., He, Y., Yi, X., et al. (2022) Rapid Start-Up of Autotrophic Shortcut Nitrification System in SBR and Microbial Community Analysis. Environmental Technology, 43, 4363-4375. [Google Scholar] [CrossRef] [PubMed]
[7] Kozlowski, J.A., Kits, K.D. and Stein, L.Y. (2016) Comparison of Nitrogen Oxide Metabolism among Diverse Ammonia-Oxidizing Bacteria. Frontiers in Microbiol-ogy, 7, 1090. [Google Scholar] [CrossRef] [PubMed]
[8] Young, M.N., Boltz, J., Rittmann, B.E., et al. (2022) Thermodynamic Analysis of Intermediary Metabolic Steps and Nitrous Oxide Production by Ammoni-um-Oxidizing Bacteria. Environmental Science & Technology, 56, 12532-12541. [Google Scholar] [CrossRef] [PubMed]
[9] Jung, M.-Y., Gwak, J.-H., Rohe, L., et al. (2019) Indications for Enzymatic Denitrification to N2O at Low pH in an Ammonia-Oxidizing Archaeon. ISME Journal, 13, 2633-2638. [Google Scholar] [CrossRef] [PubMed]
[10] Liu, S., Han, P., Hink, L., et al. (2017) Abiotic Conversion of Extracellular NH2OH Contributes to N2O Emission during Ammonia Oxidation. Environmental Science & Tech-nology, 51, 13122-13132. [Google Scholar] [CrossRef] [PubMed]
[11] Xia, Z., Wang, Q., She, Z., et al. (2019) Nitrogen Removal Pathway and Dynamics of Microbial Community with the Increase of Salinity in Simultaneous Nitrification and Denitrification Process. Science of the Total Environment, 697, Article ID: 134047. [Google Scholar] [CrossRef] [PubMed]
[12] Liu, H., Liu, D., Huang, Z., et al. (2023) Bioaugmentation Reconstructed Nitrogen Metabolism in Full-Scale Simultaneous Partial Nitrification-Denitrification, Anammox and Sulfur-Dependent Nitrite/Nitrate Reduction (SPAS). Bioresource Technology, 367, Article ID: 128233. [Google Scholar] [CrossRef] [PubMed]
[13] Li, J., Peng, Y., Yang, S., et al. (2022) Successful Ap-plication of Anammox Using the Hybrid Autotrophic-Heterotrophic Denitrification Process for Low-Strength Wastewater Treatment. Environmental Science & Technology, 56, 13964-13974. [Google Scholar] [CrossRef] [PubMed]
[14] Qiu, Y., Ji, Y., Tian, Y., et al. (2023) Engineering Demonstration of the Remediation of Urban Water Using a Novel MES Enhanced Ecological Floating Bed: From Construction to Long-Term Performance. Chemical Engineering Journal, 454, Article ID: 140024. [Google Scholar] [CrossRef
[15] Lu, J., Wang, M., Wei, J., et al. (2023) Electrolysis-Integrated Constructed Wetland with Pyrite Filler for Simultaneous Enhanced Phosphorus and Nitrogen Removal. Chemical Engineering Journal, 451, Article ID: 138542. [Google Scholar] [CrossRef
[16] Cao, Q., Chen, Y., Li, X., et al. (2023) Low C/N Promotes Stable Partial Nitrification by Enhancing the Cooperation of Functional Microorganisms in Treating High-Strength Ammonium Landfill Leachate. Journal of Environmental Management, 329, Article ID: 116972. [Google Scholar] [CrossRef] [PubMed]
[17] Tang, W., Chen, D., Phillips, O.L., et al. (2018) Effects of Long-Term Increased N Deposition on Tropical Montane Forest Soil N-2 and N2O Emissions. Soil Biology & Bio-chemistry, 126, 194-203. [Google Scholar] [CrossRef
[18] Stange, C.F., Spott, O., Arriaga, H., et al. (2013) Use of the Inverse Abundance Approach to Identify the Sources of NO and N2O Release from Spanish Forest Soils under Oxic and Hypoxic Conditions. Soil Biology & Biochemistry, 57, 451-458. [Google Scholar] [CrossRef
[19] Jansen-Willems, A.B., Lanigan, G.J., Clough, T.J., et al. (2016) Long-Term Elevation of Temperature Affects Organic N Turnover and Associated N2O Emissions in a Permanent Grassland Soil. Soil, 2, 601-614. [Google Scholar] [CrossRef
[20] Su, P., Gao, C., Zhang, X., et al. (2023) Microplastics Stimulated Nitrous Oxide Emissions Primarily through Denitrification: A Meta-Analysis. Journal of Hazardous Materials, 445, Article ID: 130500. [Google Scholar] [CrossRef] [PubMed]
[21] Antileo, C., Jaramillo, F., Candia, O., et al. (2022) Long-Term Nitrite-Oxidizing Bacteria Suppression in a Continuous Activated Sludge System Exposed to Frequent Changes in pH and Oxygen Set-Points. Journal of Environmental Management, 318, Article ID: 115545. [Google Scholar] [CrossRef] [PubMed]
[22] Wrage-Moennig, N., Horn, M.A., Well, R., et al. (2018) The Role of Nitrifier Denitrification in the Production of Nitrous Oxide Revisited. Soil Biology & Biochemistry, 123, 3-16. [Google Scholar] [CrossRef
[23] Duan, P., Song, Y., Li, S., et al. (2019) Responses of N2O Production Pathways and Related Functional Microbes to Temperature across Greenhouse Vegetable Field Soils. Geoderma, 355, Article ID: 113904. [Google Scholar] [CrossRef
[24] Lin, H., Yuan, Q., Yu, Q., et al. (2022) Plants Mitigate Nitrous Oxide Emissions from Antibiotic-Contaminated Agricultural Soils. Environmental Science & Technology, 56, 4950-4960. [Google Scholar] [CrossRef] [PubMed]
[25] Li, Q., Hou, Z., Huang, X., et al. (2023) Methanation and Chemolitrophic Nitrogen Removal by an Anaerobic Membrane Bioreactor Coupled Partial Nitri-fication and Anammox. Frontiers of Environmental Science & Engineering, 17, 68. [Google Scholar] [CrossRef
[26] Zhao, L., Fu, G., Pang, W., et al. (2023) A Novel Autotrophic Denitrification and Nitrification Integrated Constructed Wetland Process for Marine Aquaculture Wastewater Treatment. Chemosphere, 321, Article ID: 138157. [Google Scholar] [CrossRef] [PubMed]
[27] Wang, Z., Gao, J., Zhao, Y., et al. (2023) Deci-phering the Coupling of Partial Nitrification/Anammox and Sulfur Autotrophic Denitrification: Microbial Metabo-lism and Antibiotic Resistance Genes Propagation. Chemical Engineering Journal, 452, Article ID: 139176. [Google Scholar] [CrossRef
[28] Parn, J., Verhoeven, J.T.A., Butterbach-Bahl, K., et al. (2018) Nitrogen-Rich Organic Soils under Warm Well-Drained Conditions Are Global Nitrous Oxide Emission Hotspots. Nature Communications, 9, 1135. [Google Scholar] [CrossRef] [PubMed]
[29] Kool, D.M., Wrage, N., Zechmeister-Boltenstern, S., et al. (2010) Nitrifier Denitrification Can Be a Source of N2O from Soil: A Revised Approach to the Dual-Isotope La-belling Method. European Journal of Soil Science, 61, 759-772. [Google Scholar] [CrossRef
[30] Zuo, J., Xu, L., Guo, J., et al. (2023) Microbial Community Structure Analyses and Cultivable Denitrifier Isolation of Myriophyllum aquaticum Constructed Wet-land under Low C/N Ratio. Journal of Environmental Sciences, 127, 30-41. [Google Scholar] [CrossRef] [PubMed]
[31] Zhang, Q., Zheng, J., Zhao, L., et al. (2023) Succession of Mi-crobial Communities Reveals the Inevitability of Anammox Core in the Development of Anammox Processes. Bi-oresource Technology, 371, Article ID: 128645. [Google Scholar] [CrossRef] [PubMed]
[32] Friedl, J., Scheer, C., De Rosa, D., et al. (2021) Sources of Nitrous Oxide from Intensively Managed Pasture Soils: The Hole in the Pipe. Environmental Research Letters, 16, Article ID: 065004. [Google Scholar] [CrossRef
[33] Lv, Y.-T., Chen, X., Zhang, X., et al. (2023) Intermolecular Adhesion Forces Explain the Formation of Denitrifying Granular Sludge Driven by Acidic pH under Ambient Temperature. Chemical Engineering Journal, 454, Article ID: 140314. [Google Scholar] [CrossRef
[34] Wang, H., Yan, Z., Ju, X., et al. (2023) Quantifying Nitrous Oxide Production Rates from Nitrification and Denitrification under Various Moisture Conditions in Agricultural Soils: Laboratory Study and Literature Synthesis. Frontiers in Microbiology, 13, Article ID: 1110151. [Google Scholar] [CrossRef] [PubMed]
[35] Qu, Z., Wang, J., Almoy, T., et al. (2014) Excessive Use of Nitrogen in Chinese Agriculture Results in High N2O/(N2O + N-2) Product Ratio of Denitrification, Primarily Due to Acidification of the Soils. Global Change Biology, 20, 1685-1698. [Google Scholar] [CrossRef] [PubMed]
[36] Li, L., Yang, M., Li, J., et al. (2022) Potential Denitrification Activity Response to Long-Term Nitrogen Fertilization—A Global Meta-Analysis. Journal of Cleaner Production, 336, Ar-ticle ID: 130451. [Google Scholar] [CrossRef
[37] Chen, Z., White, J.F., Malik, K., et al. (2022) Soil Nutrient Dynamics Relate to Epichloe? Endophyte Mutualism and Nitrogen Turnover in a Low Nitrogen Environment. Soil Biology & Biochemistry, 174, Article ID: 108832. [Google Scholar] [CrossRef
[38] Aldossari, N. and Ishii, S. (2021) Isolation of Cold-Adapted Nitrate-Reducing Fungi That Have Potential to Increase Nitrate Removal in Woodchip Bioreactors. Journal of Ap-plied Microbiology, 131, 197-207. [Google Scholar] [CrossRef] [PubMed]
[39] Zou, Y., Hirono, Y., Yanai, Y., et al. (2014) Isotopomer Analysis of Nitrous Oxide Accumulated in Soil Cultivated with Tea (Camellia sinensis) in Shizuoka, Central Japan. Soil Biology & Biochemistry, 77, 276-291. [Google Scholar] [CrossRef
[40] Liu, C., Guo, Z., Zhang, H., et al. (2023) Single-Cell Raman Spectra Reveals the Cytochrome c-Mediated Electron Transfer in Nanoscale Zero-Valent Iron Coupled Denitrifica-tion Process. Chemical Engineering Journal, 454, Article ID: 140241. [Google Scholar] [CrossRef
[41] Su, Q., Domingo-Felez, C., Jensen, M.M., et al. (2019) Abiotic Nitrous Oxide (N2O) Production Is Strongly pH Dependent, but Contributes Little to Overall N2O Emissions in Bi-ological Nitrogen Removal Systems. Environmental Science & Technology, 53, 3508-3516. [Google Scholar] [CrossRef] [PubMed]
[42] Onley, J.R., Ahsan, S., Sanford, R.A., et al. (2018) Denitrifica-tion by Anaeromyxobacter dehalogenans, a Common Soil Bacterium Lacking the Nitrite Reductase Genes nirS and nirK. Applied and Environmental Microbiology, 84, 1985. [Google Scholar] [CrossRef
[43] Liu, S., Berns, A.E., Vereecken, H., et al. (2017) Interactive Ef-fects of MnO2, Organic Matter and pH on Abiotic Formation of N2O from Hydroxylamine in Artificial Soil Mixtures. Scientific Reports, 7, Article No. 39590. [Google Scholar] [CrossRef] [PubMed]
[44] Heil, J., Vereecken, H. and Brueggemann, N. (2016) A Review of Chemical Reactions of Nitrification Intermediates and Their Role in Nitrogen Cycling and Nitrogen Trace Gas Formation in Soil. European Journal of Soil Science, 67, 23-39. [Google Scholar] [CrossRef
[45] Buessecker, S., Tylor, K., Nye, J., et al. (2019) Effects of Sterilization Techniques on Chemodenitrification and N2O Production in Tropical Peat Soil Microcosms. Biogeosciences, 16, 4601-4612. [Google Scholar] [CrossRef
[46] Duan, P., Shen, H., Jiang, X., et al. (2020) The Contributions of Hydroxylamine and Nitrite to NO and N2O Production in Alkaline and Acidic Vegetable Soils. Journal of Soils and Sediments, 20, 2903-2911. [Google Scholar] [CrossRef
[47] Wei, J., Zhang, X., Xia, L., et al. (2022) Role of Chemical Reactions in the Nitrogenous Trace Gas Emissions and Nitrogen Retention: A Meta-Analysis. Science of the Total Environment, 808, Article ID: 152141. [Google Scholar] [CrossRef] [PubMed]
[48] Yin, Y., Yang, C., Li, M., et al. (2021) Research Progress and Prospects for Using Biochar to Mitigate Greenhouse Gas Emissions during Composting: A Review. Science of the Total Environment, 798, Article ID: 149294. [Google Scholar] [CrossRef] [PubMed]
[49] Sun, H., Yi, Z., Jeyakumar, P., et al. (2022) Citric Acid Modified Biochar Application at a Low Dosage Can Synchronically Mitigate the Nitrogenous Gas Pollutants Emis-sion from Rice Paddy Soils. Environmental Pollution, 312, Article ID: 120068. [Google Scholar] [CrossRef] [PubMed]
[50] Chu, L., Hennayake, H.M.K.D. and Sun, H. (2019) Biochar Effectively Reduces Ammonia Volatilization from Nitrogen-Applied Soils in Tea and Bamboo Plantations. Phy-ton-International Journal of Experimental Botany, 88, 261-267. [Google Scholar] [CrossRef
[51] Yang, F., Cao, X., Gao, B., et al. (2015) Short-Term Effects of Rice Straw Biochar on Sorption, Emission, and Transformation of Soil NH4+-N. Environmental Science and Pollution Research, 22, 9184-9192. [Google Scholar] [CrossRef] [PubMed]
[52] Kuo, Y.-L., Lee, C.-H. and Jien, S.-H. (2020) Reduction of Nutrient Leaching Potential in Coarse-Textured Soil by Using Biochar. Water, 12, 2012. [Google Scholar] [CrossRef
[53] Chen, X., Yang, S.-H., Jiang, Z.-W., et al. (2021) Biochar as a Tool to Reduce Environmental Impacts of Nitrogen Loss in Water-Saving Irrigation Paddy Field. Journal of Cleaner Pro-duction, 290, Article ID: 125811. [Google Scholar] [CrossRef
[54] Cao, T., Meng, J., Liang, H., et al. (2017) Can Biochar Provide Ammonium and Nitrate to Poor Soils? Soil Column Incubation. Journal of Soil Science and Plant Nutrition, 17, 253-265. [Google Scholar] [CrossRef
[55] Borchard, N., Schirrmann, M., Luz Cayuela, M., et al. (2019) Biochar, Soil and Land-Use Interactions That Reduce Nitrate Leaching and N2O Emissions: A Me-ta-Analysis. Science of the Total Environment, 651, 2354-2364. [Google Scholar] [CrossRef] [PubMed]
[56] Xie, Y., Dong, C., Chen, Z., et al. (2021) Successive Biochar Amendment Affected Crop Yield by Regulating Soil Nitrogen Functional Microbes in Wheat-Maize Rotation Farmland. Environmental Research, 194, Article ID: 110671. [Google Scholar] [CrossRef] [PubMed]
[57] Fallah, N., Yang, Z., Tayyab, M., et al. (2021) Depth-Dependent Influence of Biochar Application on the Abundance and Community Structure of Diazotrophic under Sugarcane Growth. PLOS ONE, 16, e0253970. [Google Scholar] [CrossRef] [PubMed]
[58] Li, P., Peng, Y., Wang, S., et al. (2022) N2O Emission from Partial Nitrification and Full Nitrification in Domestic Wastewater Treatment Process. Water, 14, 3195. [Google Scholar] [CrossRef
[59] Li, F., Liang, X., He, S., et al. (2020) Biochar Slows Gross Nitrifica-tion and Gasses N Emission via Lower Autotrophic Nitrification in Paddy Soils. Journal of Soils and Sediments, 20, 629-640. [Google Scholar] [CrossRef
[60] Zhao, J., Zhao, J., Yang, W., et al. (2022) Mechanisms of NO and N2O Production by Enriched Nitrifying Sludge in a Sequencing Batch Reactor: Effects of Hydroxylamine. Journal of Environmental Management, 316, Article ID: 115237. [Google Scholar] [CrossRef] [PubMed]
[61] Chen, X., Ni, B.-J. and Sin, G. (2019) Nitrous Oxide Production in Autotrophic Nitrogen Removal Granular Sludge: A Modeling Study. Biotechnology and Bioengi-neering, 116, 1280-1291. [Google Scholar] [CrossRef] [PubMed]
[62] Han, P., Wu, D., Sun, D., et al. (2021) N2O and NOy Production by the Comammox Bacterium Nitrospira inopinata in Comparison with Canonical Ammonia Oxidizers. Water Research, 190, Article ID: 116728. [Google Scholar] [CrossRef] [PubMed]
[63] Xie, J., Yan, J., He, H., et al. (2021) Evaluation of the Key Factors to Dominate Aerobic Ammonia-Oxidizing Archaea in Wastewater Treatment Plant. International Biodete-rioration & Biodegradation, 164, Article ID: 105289. [Google Scholar] [CrossRef
[64] Zhang, X., Duan, P., Wu, Z., et al. (2019) Aged Biochar Stimulated Ammonia-Oxidizing Archaea and Bacteria-Derived N2O and NO Production in an Acidic Vegetable Soil. Science of the Total Environment, 687, 433-440. [Google Scholar] [CrossRef] [PubMed]
[65] Wang, M., Yang, M., Fan, T., et al. (2023) Activating Soil Nitrification by Co-Application of Peanut Straw Biochar and Organic Fertilizer in a Rare Earth Mining Soil. Science of the Total Environment, 866, Article ID: 161506. [Google Scholar] [CrossRef] [PubMed]
[66] Fan, H., Shen, Z., Wang, X., et al. (2022) NO Reduction Reaction by Kiwi Biochar-Modified MnO2 Denitrification Catalyst: Redox Cycle and Reaction Process. Catalysts, 12, 870. [Google Scholar] [CrossRef
[67] Ahmed, M., Ahmad, S., Fayyazul, H., et al. (2019) In-novative Processes and Technologies for Nutrient Recovery from Wastes: A Comprehensive Review. Sustainability, 11, 4938. [Google Scholar] [CrossRef
[68] Viaene, J., Peiren, N., Vandamme, D., et al. (2023) Screening tests for N Sorption Allow to Select and Engineer Biochars for N Mitigation during Biomass Processing. Waste Management, 155, 230-239. [Google Scholar] [CrossRef] [PubMed]
[69] Wang, B., Gan, F., Dai, Z., et al. (2021) Air Oxidation Coupling NH3 Treatment of Biomass Derived Hierarchical Porous Biochar for Enhanced Toluene Removal. Journal of Hazardous Materials, 403, Article ID: 123995. [Google Scholar] [CrossRef] [PubMed]
[70] Cao, X., Reichel, R. and Bruggemann, N. (2022) Fenton Oxidation of Biochar Improves Retention of Cattle Slurry Nitrogen. Journal of Environmental Quality, 51, 1319-1326. [Google Scholar] [CrossRef] [PubMed]
[71] Zhang, X., Jiao, Y., Wang, B., et al. (2023) Biochar Amendments and Climate Warming Affected Nitrification Associated N2O and NO Production in a Vegetable Field. Journal of Environmental Management, 330, Article ID: 117178. [Google Scholar] [CrossRef] [PubMed]
[72] Saggar, S., Jha, N., Deslippe, J., et al. (2013) Denitrifi-cation and N2O:N-2 Production in Temperate Grasslands: Processes, Measurements, Modelling and Mitigating Negative Impacts. Science of the Total Environment, 465, 173-195. [Google Scholar] [CrossRef] [PubMed]
[73] Aamer, M., Shaaban, M., Hassan, M.U., et al. (2020) Biochar Mitigates the N2O Emissions from Acidic Soil by Increasing the nosZ and nirK Gene Abundance and Soil pH. Journal of Environmental Management, 255, Article ID: 109891. [Google Scholar] [CrossRef] [PubMed]
[74] Liu, N., Liao, P., Zhang, J., et al. (2020) Characteristics of Denitrification Genes and Relevant Enzyme Activities in Heavy-Metal Polluted Soils Remediated by Biochar and Compost. Science of the Total Environment, 739, Article ID: 139987. [Google Scholar] [CrossRef] [PubMed]
[75] Wang, X., Dan, Y., Diao, Y., et al. (2022) Transport Characteristics of Polystyrene Microplastics in Saturated Porous Media with Biochar/Fe3O4-Biochar under Various Chemical Conditions. Science of the Total Environment, 847, Article ID: 157576. [Google Scholar] [CrossRef] [PubMed]
[76] Pan, Y., She, D., Chen, X., et al. (2021) Elevation of Biochar Application as Regulator on Denitrification/NH3 Volatilization in Saline Soils. Environmental Science and Pollution Research, 28, 41712-41725. [Google Scholar] [CrossRef] [PubMed]
[77] Zhang, X., Zhang, J., Song, M., et al. (2022) N2O and NO Production and Functional Microbes Responding to Biochar Aging Process in an Intensified Vegetable Soil. Envi-ronmental Pollution, 307, Article ID: 119491. [Google Scholar] [CrossRef] [PubMed]