PI  >> Vol. 1 No. 2 (November 2012)

    镧对铽荧光增强效应测定药物加替沙星胶囊中的加替沙星含量
    Determination the Gatifloxacin Drug Content in Gatifloxacin Capsule by Effect of Lanthanum on Terbium Fluorescence Enhancement Effect

  • 全文下载: PDF(333KB) HTML   XML   PP.15-20   DOI: 10.12677/PI.2012.12004  
  • 下载量: 2,430  浏览量: 11,175   科研立项经费支持

作者:  

赵悦辉,李烨,李刚,宋冬雪,AbdullahAbdu Ahmed ,郑极慧,吕玉光:佳木斯大学药学院;
谭博,马丽丽:佳木斯市食品药品检验所;
王冠乔:沈阳药科大学

关键词:
稀土离子喹诺酮类加替沙星荧光分光光度法Rare Earth Ions; Quinolone; Gatifloxacin; Fluorescence Spectrophotometry

摘要:

探讨第四代喹诺酮类药物加替沙星与稀土离子铽离子形成的配合物的荧光特性。应用荧光分光光度计测定药物加替沙星与稀土离子铽离子形成的配合物的荧光强度,通过加入表面活性剂、加入共发光离子La3+、改变溶液的pH等增敏方法选择最佳条件。镧对该体系具有较强的荧光增强效应,最佳的pH值为7.5,选用0.1 mol·L–1 NH4Cl-HCl效果最佳,加替沙星的最佳浓度为1.0 × 10–6 mol·L–1。对荧光新体系的荧光增强作用的机理进行了初步探讨,最终建立了Tb3+-La3+-GFLX-SDS新体系,上述研究对于稀土元素分析、生物和药物分析都具有潜在的应用价值,为稀土和喹诺酮类药物配合物的相关研究提供了一定的理论和实验基础,可以用于测定人工合成样品和制剂的含量,该方法选择性好,灵敏度高,结果令人满意。

To study fluorescence of the forth generation of quinolones gatifloxacin and the rare earth ion complexes. Apply fluorescence spectrophotometry detects the fluorescence intensity of gatifloxacin and the rare earth ion com-plexes. Through the surface active agent, change pH and the emitting ions were added to enhance the fluorescence in-tensity to achieve the establishment of a high sensitivity, high selectivity of the fluorescent system. Tb3+-GFLX-SDS system can be strongly emitted by La3+, the best pH 7.5, GFLX 1.0 × 10–6 mol·L–1, 0.1 mol·L–1 NH4Cl-HCl. The fluo-rescence enhancement mechanism was discussed. Tb3+-La3+-GFLX-SDS new system was established. Rare earth ele-ment analysis for the study, biological and pharmaceutical analysis has potential applications for rare earth complexes of a quinolone-related research provide a theoretical and experimental basis. A test method based on this co-lumines- cence system was applied to the determination of the medicine content with satisfactory results.

文章引用:
赵悦辉, 李烨, 谭博, 马丽丽, 王冠乔, 李刚, 宋冬雪, AbdullahAbdu Ahmed, 郑极慧, 吕玉光. 镧对铽荧光增强效应测定药物加替沙星胶囊中的加替沙星含量[J]. 药物资讯, 2012, 1(2): 15-20. http://dx.doi.org/10.12677/PI.2012.12004

参考文献

[1] 陈超森, 曾卓, 熊淑群. 喹诺酮类药物的研究进展[J]. 精细化工中间体, 2005, 35(5): 1-5.
[2] 陈磊, 高忠良, 刘雁等. 喹诺酮类药物母环的衍变及合成研究[J]. 精细石油化工进展, 2005, 6(11): 37-42.
[3] 王玉成, 王秀云, 郭慧元. 近年我国喹诺酮类抗菌剂的研究进展[J]. 中国医药工业杂志, 2004, 35(3): 179-185.
[4] 粱诚. 氟喹诺酮类药物生产现状与研究进展[J]. 精细与专用化学品, 2005, 13(5): 1-3.
[5] 连宁, 孙春燕, 赵慧春. 加替沙星的荧光光度法测定[J]. 分析测试学报, 2002, 21: 79-81.
[6] M. Cordoba-Borrego, M. Cordoba-Diaz, I. Bernabe and D. Cordoba-Diaz. Determination of norfloxacin by fluorescence in the presence of different antacids: Quantification of analytical interferences. Journal of Pharmaceutical and Biomedical Analysis, 1996, 8(14): 977-982.
[7] A. Navalón, O. Ballesteros, R. Blanc and J. L. Vílchez. Deter- mination of ciprofloxacin in human urine and serum samples by solid-phase spectrofluorimetry. Talanta, 2000, 5(52): 845-852.
[8] 仲宣惟. 荧光光度法测定氟罗沙星胶囊含量[J]. 天津药学, 2000, 12: 65-66.
[9] 徐莉英等. 诺氟沙星在胶束体系中的荧光分析方法[J]. 理化检验–化学分册, 2002, 38: 487-488.
[10] 王静萍, 杜黎明, 许庆琴. 环丙沙星在胶束体系中的荧光特性研究及应用[J]. 光谱学与光谱分析, 2002, 22(2): 287-288.
[11] 高建华, 陈彬, 菅军伟. 血清中盐酸洛美沙星的测定及荧光光谱特性[J]. 郑州大学学报(自然科学版), 2000, 32(4): 74-78.
[12] 颜承农, 上官风云, 潘祖亭等. 盐酸芦氟沙星的荧光光谱分析法研究[J]. 分析科学学报, 2003, 19(2): 136-138.
[13] 杜黎明, 王静萍, 范哲锋, 叶玉香. 氧氟沙星在胶束体系中的荧光特性及应用[J]. 分析化学, 2002, 30(1): 59-61.
[14] M. C. Mahedero, D. Bohoyo, F. Salinas and T. Ardila. Improve- ment sineuro Pimsensitized fiuorimetric determination of demee- locyelin and methacyeline. Journal of Pharmaceutical and Bio- medical Analysis, 2005, 37(5): 1101-1104.
[15] C. Y. Chen, C. C. Guo and W. Jiang. Rapid determination minoeyeline in Pharmaeutieal for mulations and humanne/serumsam Plesbase on the minoeyeline-euroPiurn-sodiutndode eylbenzene sulfonate lumineseenc system. Analytical Letters, 2009, 42(11): 22-42.
[16] C. X. Sun, J. H. Yang, L. Li, et al. Advances in the study of luminescence probes for proteins. Journal of Chromatography B, 2004, 803(1): 173-190.
[17] 吕玉光, 赵悦辉, 李刚, 王莹, 李烨等. 采用荧光光谱法测定谷氨酸诺氟沙星氯化钠注射液中诺氟沙星的含量[J]. 中国卫生检验杂志, 2012, 8: 1-3.
[18] M. Rizk, Y. El-Shabrawy. Fluorimetric determination of aminoglycoside antibiotics using lanthanide probe ion spectroscopy. Talanta, 1995, 42(12): 1849-1856.
[19] 童裳伦, 项光宏, 黄迪金等. 表面活性剂敏化铽离子荧光探针对氧氟沙星的测定[J]. 分析化学, 2004, 32(5): 619-621.
[20] 周静, 杜黎明, 陈彩萍. 铽-培氟沙星的荧光特性研究及培氟沙星的测定[J]. 光谱学与光谱分析, 2004, 24(6): 717-719.
[21] 杜黎明, 晋卫军, 董川等. 司帕沙星-亚硝酸荧光体系性质的研究及应用[J]. 化学通报(论文摘要), 2000, 4: 34-34.
[22] 杜黎明, 范哲锋, 郭全娥. 司帕沙星与卤素反应产物的荧光特性及其应用[J]. 分析化学, 2001, 29(3): 249-252.
[23] L. Li, J. H. Yang, X. Wu, et al. Analysis of guanosine-50-monoPHosPHate at nM level based on the columinescence effect of Tb-Gd-GMP system. Journal of Luminescence, 2004, 109(1): 31-37.
[24] C. X. Sun, J. H. Yang and L. Li. Advances in the study of luminescence probes for proteins. Journal of Chromatography B, 2004, 803(2): 173-190.
[25] L. Wang. Spectrofluorimetric determination of trace amounts of Europium(Ⅲ) ion with Lutetium(Ⅲ)-sparfloxacin-sodium do- decyl sulfate luminescence enhancement system. Analytical Sci- ences, 2004, 20(1): 1237-1239.