山东百部遗传多样性分析
Genetic Diversity of Stemona shandongensis Populations
DOI: 10.12677/BR.2013.21001, PDF, HTML, XML,  被引量 下载: 3,525  浏览: 13,779  科研立项经费支持
作者: 张 雷, 马 燕, 臧德奎：山东农业大学林学院，泰安
关键词: AFLP；山东百部；遗传多样性；居群； AFLP; Stemona shandongensis; Genetic Diversity; Population
摘要:

利用AFLP分子标记技术对山东百部4个居群120个样品进行了遗传多样性分析。8对引物组合共扩增出282条清晰可辨条带，其中多态性条带137条，多态性位点百分率48.58%；在种级水平上，Nei’s基因多样性指数为0.1441，Shannon多态性信息指数为0.2485；居群总遗传多样性(Ht)为0.1646，居群内遗传多样性为0.1682，基因分化系数为0.0210。UPGMA聚类表明，AFLP聚类结果与表型分化聚类结果一致，居群间具有一定程度的遗传分化，并通过表型性状表现出来。

Abstract: Genetic diversity and genetic structure of 120 individuals of four Stemona shandongensis popula- tions were investigated by AFLP method. A total of 282 bands were observed with 8 primer combination, in- cluding 137 polymorphic bands. The percentage of polymorphic bands was 48.58%. In species level, Nei’s gene diversity index was 0.1441; Shannon’s information index was 0.2485; total gene diversity was 0.1646; population gene diversity was 0.1682, gene differentiation coefficient was 0.0210. UPGMA cluster based on AFLP makers was similar to the cluster based on morphological traits; there was no significant correlation between genetic distance and geographic distance. The results showed that there was genetic differentiation among populations, and it could express by morphological characteristics.

文章引用：张雷, 马燕, 臧德奎. 山东百部遗传多样性分析[J]. 植物学研究, 2013, 2(1): 1-5. http://dx.doi.org/10.12677/BR.2013.21001

参考文献

[1] 臧得奎, 彭卫东. 百部属一新种[J]. 植物研究, 1996, 16(4): 413-414.
[2] Z.-H. Ji, B. E. Duyfjes. E. stemona. In: Z. Y. Wu, P. H. Raven and D. Y. Hong, Eds., Flora of China, Beijing: Science Press, St. Louis: MBG Press, 2000, 24: 70-72.
[3] 李法曾, 赵遵田, 樊守金. 山东植物精要[M]. 北京: 科学出版社, 2004.
[4] 白音, 包英华, 王文权等. 国产石斛属植物亲缘关系的AFLP分析[J]. 园艺学报, 2007, 34(6): 1569-1574.
[5] 甘娜, 谭向红, 陈其兵等. 应用RAPD标记和细胞质基因组PCR-RFLP技术研究大花蕙兰的遗传多样性[J]. 园艺学报, 2006, 33(2): 349-355.
[6] 张春雨, 陈学森, 何天明. Genetic structure of Malus sieversii population from Xinjiang, China, revealed by SSR markers [J]. 遗传学报, 2007, 34(10): 947-955.
[7] 赵庆芳, 李巧峡, 马世荣等. 青藏高原东部矮生嵩草遗传多样性的RAPD研究[J]. 生态学报, 2006, 26(8): 2494-2501.
[8] 易杨杰, 张新全, 黄琳凯等. 野生狗牙根种质遗传多样性的SRAP研究[J]. 遗传, 2008, 30(1): 94-100.
[9] 陈良华, 胡庭兴, 张帆等. 用AFLP技术分析四川核桃资源的遗传多样性[J]. 植物生态学报, 2008, 32(6): 1326-1372.
[10] 黄久香, 黄妃本, 许涵等. 海南岛青梅AFLP标记的遗传多样性[J]. 林业科学, 2008, 44(5): 46-52.
[11] G. S. H. Fang, R. Grumet. A quick and inexpensive method for removing polysaccharides from plant genomic DNA. Biotech- niques, 1992, 13: 52-57.
[12] F. C. Yeh, R. C. Yang and T. Boyle. POPGENE, microsoft windows-based freeware for population genetic analysis release 1.31. Edmonton: University of Alberta, 1999.
[13] M. Nei, W. H. Li. Mathematical model for studying genetic variation interims of restriction Endonucleases. Proceeding of the National Academy Sciences of the United States of the America, 1979, 76: 5269-5273.
[14] F. J. Rohlf. NTSYS pc2.1: Numerical taxonomy and multivariate analysis system version 2.1. New York: Applied Biostatistics Inc., 2000.
http:∥www.exetersoftware.com/cat/ntsyspc/ntsyspc.html.
[15] 张雷, 邱乾栋, 臧德奎. 山东百部的表型分化研究[J]. 山东林业科技, 2009, 5: 29-32.
[16] 洪锐民, 王昱生, 杨建华. 羊草(Leymus chinensis)等植物居群克隆表型变异和遗传变异的分子遗传–生态学分析[J]. 东北农业大学学报, 2001, 32(4): 313-319.
[17] 张雷, 邱乾栋, 臧德奎. 山东百部居群的空间分布格局[J]. 武汉植物学研究, 2009, 27(6): 617-621.
[18] Y. Naito, A. Konuma, H. Iwata, Y. Suyama, K. Seiwa, T. Okuda, S. L. Lee, N. Muhammad and Y. Tsumura. Self and inbreeding depression in seeds and seedlings of Neobalanocarpus heimii (Dipterocarpaceae). Journal of Plant Research, 2005, 118: 423- 430.