优良品种龙滩珍珠李自花结实分子机理初探

Pre-Study on the Molecular Mechanism of Self-Compatibility in Longtanzhenzhuli

摘要: 本研究主要以广西本地生长的优质的具自花结实李品种龙滩珍珠李为材料，根据李属雌蕊S-RNase基因的序列信息设计了一对特异性保守引物，对龙滩珍珠李基因组DNA进行PCR扩增，并对扩增产物进行克隆、测序以及序列分析，成功克隆出两个S-RNase基因片段，其中一个为在中国李中首次发现并命名为PsS-RNase-28，大小为727 bp，其中包含有367 bp的内含子，仅有360 bp的外显子编码序列，与黑刺李(Prunus spinosa)S-RNase S1基因(EF636467.1)同源率达到98%，与其它李S- RNase基因的同源率都达到79%以上。另一个扩增片段大小为718 bp，包含有343 bp的内含子，其中仅有375 bp的外显子编码序列，与中国李(Prunus salicina)PsS-RNase-h基因(AB084148.1)相似性达到99%，与其它李S-RNase基因的相似性都达到85%以上。这两个基因片段的获得及其后续分析有利于进一步探讨龙滩珍珠李自花结实的分子机理。

Abstract: Longtanzhenzhuli which is a new variety of plum with high quality were used as the experimental materials in this research. A specific primer was designed based on the conserved sequences of plum (Prunus salicina Lindl.) S-Rnase(S) genes, two specific fragments were isolated from Longtanzhenzhuli genome DNA with tese specific primers. The sequencing results showed that these fragments were confirmed as the plum S genes. One was first cloned from Prunus salicina and named PsS-RNase-28, with length of 727 bp, containing 367 bp intron and 360 bp Exton. Comparing with Prunus spinosa S-RNase S1 gene (EF636467.1), it showed high identity (98%), more than 79% with other plums S-RNase genes. The other fragment with 718 bp in length, containing 343 bp intron and 375 bp Exton, showed high identity (99%) with Prunus salicina PsS-RNase-h gene (AB084148.1), and more than 85% with other plums S-RNase genes. And sequences follow-up analysis of these fragments would be benefited to study on the mechanism of self-compatibility of Longtanzhenzhuli.