观察清肝利水方对高眼压大鼠眼压及视网膜神经节细胞保护作用

Protective Effects of Qing-Gan-Li-Shui Formula on Intraocular Pressure and Retinal Ganglion Cells of Glaucoma Rats

摘要: 目的：青光眼是目前致盲的第二大眼病。清肝利水方是上海名老中医邹菊生先生的经验方，以清肝利水为治则，临床治疗高眼压型青光眼取得明显疗效。本课题探究该方对高眼压大鼠眼压及视网膜神经节细胞的保护作用。方法：采用灼伤巩膜上静脉方法阻断房水回流途径建立高眼压大鼠模型，分别给予清肝利水方、清肝利水方合噻吗心安滴眼液、噻吗心安滴眼液治疗，另设模型组、假手术组。定时监测眼压变化，治疗末分离视网膜，进行H&E染色，神经节细胞计数并观察视网膜形态结构。结果：成功稳定建立高眼压大鼠模型；治疗28天后，中合西药组(9.45 ± 1.04)降眼压效果优于中药组(10.50 ± 1.98)，且同时优于西药组(10.91 ± 1.76)，且中药参与治疗组降眼压效果和缓稳定。视网膜神经节细胞计数方面，中合西药组(53.00 ± 12.33)高于中药组(47.80 ± 9.60)，高于西药组(38.00 ± 12.30)。结论：清肝利水方对高眼压大鼠眼压及视网膜神经节细胞具有保护作用。

Abstract: Objectives: Glaucoma ranks second among those that induce vision loss. Qing-Gan-Li-Shui Formula is derived from Jusheng Zou, an experienced TCM doctor, and exhibited effective for glaucoma. This project is focusing on related basic research on glaucoma rats’ intraocular pressure (IOP) and retinal ganglion cells (RCGs). Methods: Modeling by occluding episcleral veins. Groups are treated with Qing-Gan-Li-Shui Formula and Timolol Maleate drops, respectively and concurrently. Model group and sham-model group were established as control. We monitored IOP regularly, collected and stained retina with H&E dye to observe RCGs. Results: Model of high IOP was established suc-cessfully. IOP of Chi-Western Group (9.45 ± 1.04) is better than individual significantly (Chinese Medical Group: 10.50 ± 1.98; Western Medical Group: 10.91 ± 1.76). RCGs of Chi-Western Group (53.00 ± 12.33) are more than Chinese Medical Group’s (47.80 ± 9.60) and Western Medical Group’s (38.00 ± 12.30) significantly. Conclusions: Qing-Gan-Li-Shui Formula exhibited protective effects on glaucoma with reduction on IOP and inhibition on RGCs apoptosis.