兰属新材料叶绿素含量及叶绿体超微结构分析

Chlorophyll Contents and Chloroplast Ultrastructure of Chlorophyll Deficient Mutant in Cymbidum

摘要: 以组培诱变获得的兰属春剑叶色突变新材料LCS-2和亲本LCS-1为试材，分别测定叶片叶绿素和类胡萝卜素含量，并对叶片叶肉细胞进行叶绿体超微结构观察。结果表明：(1) LCS-2叶绿素含量明显低于LCS-1，叶绿素a/b的比值大于LCS-1；(2) LCS-2叶绿体数量少，形状不规则，膜结构解体，无类囊体，叶绿体内无淀粉粒，嗜锇滴较多；(3) LCS-1叶绿体较多，形状规则，基粒片层清晰，淀粉粒多而大，嗜锇滴较少。表明经组培诱变的叶色突变新材料与亲本存在明显的叶绿素含量及叶绿体结构差异。

Abstract: The varieties used in this research were Cymbidum LCS-2 with verge line pattern and Cymbidum LCS-1. Chlorophyll contents and chloroplast ultrastructure were researched. The results showed that: (1) The chlorophyll contents in LCS-2 is lower than that in LCS-1. But the ratio of chlorophyll-a to chlorophyll-b of LCS-2 was higher than that of LCS-1. (2) There was a fewer chloroplasts with abnormal shape in LCS-2 cells, in which there were no thylakoids or starch grains, but many osmiophil globules, the membrane of the chloroplast had disjointed. (3) There were more regular chloroplasts in cells of LCS-1, with more and bigger starch grains but fewer osmiophil globules. Grana lamella could be seen clearly too. The results can provide evidence for variety breeding for Cymbidum with verge line pattern lives.