阳春砂叶片受病害诱导产生更多的挥发性萜类

Leaves of Amomum villosum Lour. Produce More Volatile Terpenoids Induced by Disease

摘要: 目的：分析阳春砂病变叶片中的挥发性萜类，探究阳春砂叶片受病害刺激后挥发性萜类代谢的响应情况，为阳春砂抗病相关的萜类代谢及其抗病机理的研究奠定基础。方法：分别提取阳春砂病变叶片中健康部位和病变部位的挥发性萜类，采用GC-MS方法进行分析。结果：阳春砂叶片病变部位能检测到更多的挥发性萜类，有10种单萜和1种倍半萜仅在病变部位检测到，如葑烯、香芹醇、龙脑烯醛等，大多为含氧单萜。与健康部位相比，侧柏烯、莰烯、柠檬烯、松香芹醇的含量有显著性提高(p < 0.05)，其中柠檬烯的含量有极显著性提高(p < 0.01)，是健康部位的5.27倍。结论：阳春砂叶片受病害诱导产生更多的挥发性萜类，其种类增加，部分含量显著提高，这些挥发性萜类可能参与了阳春砂对病害的防御。

Abstract: Objective: To analyze the volatile terpenoids composition in the pathological leaves of Amomum villosum Lour. and to explore the response of metabolism of volatile terpenoids while the leaves are stimulated by pathogenic bacteria. Method: Volatile terpenoids in the healthy and diseased parts of pathological leaves of A. villosum were extracted respectively and detected by GC-MS. Result: More species of volatile terpenoids were detected in the diseased part, including ten monoterpenoids and one sesquiterpenoid, as fenchene, carveol, campholenal etc., most of which were oxygenated monoterpenoids. The contents of 3-thujene, camphene, D-limonene and pinocarveol in the diseased part were significantly higher than those in the healthy part (p < 0.05), especially the content of D-limonene in the diseased part was 5.27 times that in the health part (p < 0.01). Conclusion: Different quality and quantity volatiles terpenoids were released when the leaves of A. villosum were infected. More species of volatile terpenoids were induced and the contents of some volatile terpenoids had been significantly increased. These volatile terpenoids may be involved in the resistance to disease in A. villosum.