检测水中两种异味物质的SPME-GC-MS/MS方法

Determination of Two Odor Chemicals in Water Using SPME-GC-MS/MS Method

摘要: 本实验室探索了一种新的定量分析测定生活饮用水中2-甲基异莰醇和土臭素两种异味物质的全自动固相微萃取–三重四级杆气质联用仪法，主要对测定模式进行了优化，其他条件均相同的情况下，用dMRM模式和SIM模式进行比较测定，通过测定最低浓度点计算两种模式下的检出限和定量限，同时测定浓度为20.0 ng/L、50.0 ng/L的空白加标样品计算加标回收率。结果显示，dMRM模式下2-甲基异莰醇的检出限和定量限分别是0.82 ng/L、2.75 ng/L、回收率91.1%~107.1%，土臭素的检出限和定量限分别是0.63 ng/L、2.09 ng/L、回收率86.5%~102.0%。本实验中的方法可以高效准确的测定水中痕量异味物质，能够在检测生活饮用水中异味物质领域得以广泛的应用。

Abstract: A new fully automated method for the quantitative analysis of two odor chemicals, 2-methyliso- boreneol and geosmin, in drinking water was developed and validated in this study. The method involved solid-phase microextraction (SPME) and gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The comparative determination was conducted using the dMRM and SIM modes under the same conditions. The limit of detection (LOD) and limit of quantification (LOQ) for two target chemicals were determined by analyzing the minimum concentration points in both modes. Blank-spiking tests were also performed on standard chemicals of 20.0 ng/L and 50.0 ng/L. Our results show that the LOD and LOQ for 2-methylisogonanol were 0.82 ng/L and 2.75 ng/L, respectively. Additionally, the blank-spiking test exhibited recoveries within the range of 91.1%~107.1% when analyzed in the dMRM mode. For geosmin, the LOD and LOQ were found to be 0.63 ng/L and 2.09 ng/L, respectively, and the blank-spiking test indicated recoveries for this chemical between 86.5% and 102.0%. The method can proficiently and precisely identify trace odor compounds in drinking water, and is expected to have extensive utility.