白梨SOT基因家族成员组织表达特性的分析
Analysis of Tissue Specific Expression of SOT Gene Family in White Pear
摘要: 本文旨在鉴定出白梨山梨醇转运蛋白(SOT)基因家族成员，利用生物信息学和基因组织表达特性的分析，明确梨各组织中参与山梨醇运输的主要SOT基因。基于全基因组测序结果，采用生物信息学方法鉴定出苹果、白梨、桃、梅、草莓和黑树莓6个蔷薇科果树物种SOT基因家族序列信息，并进行基因结构、理化特性等相关分析，并利用实时荧光定量PCR(qRT-PCR)技术测定PbSOTs基因在白梨各个器官组织中的表达特性。从六个蔷薇科果树物种分离出SOT基因家族成员62个，其中，PbSOT11、PbSOT12、PbSOT14、PbSOT16、PbSOT23和PbSOT24在白梨各组织中几乎都不表达；PbSOT5仅在花中表达；PbSOT4在白梨各组织中基本也都不表达，PbSOT7/8仅在茎、花、果中表达；PbSOT10和PbSOT13在白梨各组织中同样都不表达，PbSOT9仅在果实中表达；PbSOT6和PbSOT20在所有组织中均表达；PbSOT1/2仅在根、茎、花和种子中表达，而PbSOT3和PbSOT19/21和PbSOT22在根、茎、花、叶和果实中都表达，但在种子中基本不表达。PbSOT20是梨山梨醇“源–库”迁移过程各组织的主要调控基因。而PbSOT6和PbSOT19/21是根组织中山梨醇转运主要调控基因。
Abstract: The purpose of this paper is to identify sorbitol transporter (SOT) gene family members in white pear and the major SOT genes for sorbitol transport in pear tissues, using bioinformatics and the analysis of gene expression characteristic. Based on the sequenced results of whole genome from Malus domestica, Fragaria vesca, Prunus mume, Prunus persica, Pyrus bretschneideri and Rubus occidentalis, the gene sequences, gene structure, physical and chemical properties were analyzed by the method of bioinformation, the expression characteristics of PbSOTs gene in the various or-gans of white pear were analyzed by real-time fluorescence quantitative PCR (qRT-PCR). In this study, a total of 62 SOT genes were identified from six Rosaceae species. PbSOT11, PbSOT12, PbSOT14, PbSOT16, PbSOT23 and PbSOT24 were almost not expressed in Chinese white pear tissues; PbSOT5 was only expressed in flowers; PbSOT4 was also almost not expressed in all tissues of Chinese white pear, and PbSOT7/8 was only expressed in stem, flower and fruit; PbSOT10 and PbSOT13 were equally unexpressed in the white pear tissues, PbSOT9 was only expressed in fruit; PbSOT6 and PbSOT20 were expressed in all tissues; PbSOT1/2 was just expressed in root, stem, flower, and seed, and PbSOT3 was expressed in roots, stems, flowers, leaves and fruits, but not in seeds, as well as PbSOT19/21 and PbSOT22. PbSOT20 is the main regulatory gene for sorbitol transportation from source to sink in all kinds of tissues in pear, while PbSOT6 and PbSOT19/21 are the possible regulatory genes for sorbitol transportation in root tissue.
文章引用：余彩云, 谷超, 张绍铃. 白梨SOT基因家族成员组织表达特性的分析[J]. 植物学研究, 2018, 7(5): 496-506. https://doi.org/10.12677/BR.2018.75060

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