电针对兔激素性股骨头坏死血清HIF-1α、VEGF、BMP2、Osterix的影响

Effect of Electroacupuncture on Serum HIF-1α, VEGF, BMP2, and Osterix in Rabbits with Steroid-Induced Femoral Head Necrosis

摘要: 目的：观察电针对兔激素性股骨头坏死血清中成血管因子HIF-1α、VEGF；成骨因子BMP2、Osterix；H型血管标志物CD31；骨破坏相关因子MMP13的影响，探索电针治疗激素性股骨头坏死的治疗作用及机制。方法：选取健康雄性新西兰大白兔18只，随机分成正常组、模型组、电针组(电针环跳穴、髀关穴)，每组6只。运用qRT-PCR试剂盒检测兔血清中HIF-1α、VEGF、BMP2、Osterix含量。运用免疫荧光染色检测H型血管标志物CD31、骨破坏相关因子MMP13表达情况。结果：HIF-1α、VEGF、BMP2、Osterix的含量，模型组浓度明显低于正常组(P < 0.05)，电针组浓度明显高于模型组(P < 0.05)。免疫荧光染色中，模型组CD31的表达明显低于正常组，电针组CD31的表达明显高于模型组；模型组的MMP13表达量明显高于正常组，电针组的MMP13表达量明显低于模型组。结论：大量糖皮质激素的运用可下调VEGF、HIF-α、BMP2、Osterix、CD31的表达，从而造成骨质破坏和血管破坏，导致股骨头坏死。电针能调控成血管相关因子VEGF、HIF-α，成骨相关因子BMP2、Osterix，H型血管标志物CD31，使VEGF、HIF-α、BMP2、Osterix、CD31浓度均增高，促进股骨头内血管新生，骨破坏相关因子MMP13表达量下降，从而增强骨组织自身修复能力。这可能是电针治疗激素性股骨头坏死的机制之一。

Abstract: Objective: To observe the effect of electroacupuncture on angiogenic factors HIF-1α and VEGF; osteogenic factors BMP2 and Osterix; H-type vascular marker CD31; and bone destruction-related factor MMP13 in the serum of rabbits with steroid-induced femoral head necrosis, and to explore the therapeutic effect and mechanism of electroacupuncture in the treatment of steroid-induced femoral head necrosis. Methods: Eighteen healthy male New Zealand white rabbits were randomly divided into a normal group, a model group, and an electroacupuncture group (electroacupuncture at Huantiao acupoint and Biguan acupoint), with 6 rabbits in each group. The levels of HIF-1α, VEGF, BMP2, and Osterix in rabbit serum were detected by qRT-PCR kit. Immunofluorescence staining was used to detect the expression of H-type vascular marker CD31 and bone destruction-related factor MMP13. Results: The concentrations of HIF-1α, VEGF, BMP2, and Osterix in the model group were significantly lower than those in the normal group (P < 0.05), and the concentrations in the electroacupuncture group were significantly higher than those in the model group (P < 0.05). In immunofluorescence staining, the expression of CD31 in the model group was significantly lower than that in the normal group, and the expression of CD31 in the electroacupuncture group was significantly higher than that in the model group; the expression of MMP13 in the model group was significantly higher than that in the normal group, and the expression of MMP13 in the electroacupuncture group was significantly lower than that in the model group. Conclusion: The use of a large amount of glucocorticoids can downregulate the expression of VEGF, HIF-α, BMP2, Osterix, and CD31, thereby causing bone destruction and vascular destruction, leading to femoral head necrosis. Electroacupuncture can regulate angiogenesis-related factors VEGF, HIF-α, osteogenic-related factors BMP2, Osterix, and H-type vascular marker CD31, increasing the concentrations of VEGF, HIF-α, BMP2, Osterix, and CD31, promoting angiogenesis in the femoral head, and reducing the expression of bone destruction-related factor MMP13, thereby enhancing the self-repair ability of bone tissue. This may be one of the mechanisms of electroacupuncture treatment of steroid-induced femoral head necrosis.