机械离子通道蛋白Piezo1在咬合创伤大鼠牙周组织中的表达及功能研究
Expression and Functional Study of Mechanosensitive Ion Channel Protein Piezo1 in Periodontal Tissues of Rats with Occlusal Trauma
DOI: 10.12677/acm.2025.1592601, PDF,   
作者: 周佳瑶：重庆医科大学口腔医学院，重庆
关键词: 咬合创伤；牙周组织；Piezo1；Occlusal Trauma； Periodontal Tissue； Piezo1
摘要: 目的：通过研究机械离子通道Piezo1在咬合创伤大鼠牙周组织与正常大鼠牙周组织中的表达差异情况，探讨其在咬合创伤性牙周炎中发生发展的作用。方法：12周龄雄性SD大鼠30只，随机分为5组(其中，1个正常对照组和4个实验组)，每组6只。正常对照组不作任何处理，实验组通过在大鼠左上颌第一磨牙颌面粘接树脂并内置不锈钢丝形成高出颌面1 mm的树脂层以建立咬合创伤实验动物模型，分别于建模后1、3、7、14天处死各组大鼠，收集大鼠咬合创伤侧颌骨组织，通过HE、TRAP染色分析咬合创伤牙周组织形态变化，同时应用免疫组织化学染色法、蛋白质印迹法(Western blot)检测Piezo1蛋白在各组大鼠牙周膜中的表达变化。结果：随着咬合创伤时间的增加，Piezo1在咬合创伤组大鼠牙周组织中阳性表达呈显著上升趋势。同时，与正常对照组相比，piezo1在咬合创伤大鼠牙周组织中显著高表达。结论：机械离子通道蛋白Piezo1可能在大鼠咬合创伤性牙周炎发生发展过程中发挥重要作用。
Abstract: Objective: By investigating the expression differences of the mechanosensitive ion channel Piezo1 in the periodontal tissues of rats with occlusal trauma versus normal rats, this study explores its role in the development of traumatic occlusal periodontitis. Methods: Thirty 12-week-old male SD rats were randomly divided into 5 groups (including 1 normal control group and 4 experimental groups), with 6 rats in each group. The normal control group received no treatment, while the experimental groups underwent resin bonding on the occlusal surface of the left maxillary first molar with embedded stainless steel wire to create a 1 mm-high resin layer, establishing an occlusal trauma animal model. Rats in each group were euthanized at 1, 3, 7, and 14 days post-modeling, and the occlusal trauma-side jawbone tissues were collected. Hematoxylin-eosin (HE) and tartrate-resistant acid phosphatase (TRAP) staining were used to analyze morphological changes in the periodontal tissues. Immunohistochemical staining and Western blot were employed to detect Piezo1 protein expression changes in the periodontal ligament of each group. Results: With prolonged occlusal trauma duration, Piezo1 expression in the periodontal tissues of the trauma groups showed a significant upward trend. Additionally, compared with the normal control group, Piezo1 expression was significantly higher in the periodontal tissues of rats with occlusal trauma. Conclusion: The mechanosensitive ion channel protein Piezo1 may play a crucial role in the development of traumatic occlusal periodontitis in rats.
文章引用：周佳瑶. 机械离子通道蛋白Piezo1在咬合创伤大鼠牙周组织中的表达及功能研究[J]. 临床医学进展, 2025, 15(9): 1122-1130. https://doi.org/10.12677/acm.2025.1592601

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