新斯的明经穴位注射对大鼠关节疼痛的镇痛作用及机制研究

Analgesic Efficacy and Mechanism of Acupoint Injection of Neostigmine in a Rat Model of Joint Pain

摘要: 目的：新斯的明可经关节腔注射发挥外周镇痛作用，但关节注射存在技术难度和并发症风险。本研究目的为制备大鼠关节疼痛动物模型，经穴位注射新斯的明，分析镇痛效果及作用机制，为新斯的明外周注射治疗关节疼痛的临床应用提供临床前研究依据。方法：采用雄性Wistar大鼠经踝关节注射单碘醋酸钠生理盐水溶液制备关节疼痛模型，经行为学观察及痛阈测量确认模型制备成功。实验组经后三里穴位注射新斯的明，对照组注射等体积生理盐水。注射后测量两组实验动物舔足次数和热辐射痛阈，取血清样本测量前炎症因子白介素1-1β (IL-1β)和肿瘤坏死因子-α (TNF-α)水平。结果：与对照组相比，实验组动物经穴位新斯的明注射后的舔足次数减少，热辐射痛阈提高，血清IL-1β和TNF-α水平降低，两组间上述指标结果差异具有统计学意义(p < 0.01)。结论：研究表明经穴位于关节周围注射新斯的明，可提高关节疼痛实验动物热辐射痛阈，降低体内前炎症因子表达水平，产生镇痛作用。

Abstract: Objective: Neostigmine could provide peripheral analgesic effects via intra-articular injection, but with obvious technical difficulty and potential complications. The purpose of this study is to inves-tigate the analgesic effect and mechanism of acupoint injection of neostigmine in a rat model of joint pain. Methods: Animal models of ankle joint pain were prepared via intra-articular injection of monosodium iodoacetate in male Wistar rats. The experimental group received acupoint injection of neostigmine while the control group received acupoint injection of normal saline. The numbers of hind paw licking and radiation pain threshold were recorded, and serum IL-1β and TNF-α were measured using ELISA. Results: Compared with the control group, the animals in the experimental group presented less hind paw licking, higher radiation threshold and lower levels of serum IL-1β and TNF-α (p < 0.01). Conclusion: Acupoint injection of neostigmine elevated the radiation pain threshold and lowered the level of proinflammatory pain mediators, providing effective analgesia in the rat model of joint pain.