阿克苏地区胡杨树腐烂病菌系统发育分析

Phylogenetic Analysis of Rot Pathogens of Populus euphratica in Aksu Region

摘要: 以阿克苏地区农田防护林胡杨树(Populus euphratica)腐烂病病菌为研究材料，采用常规组织分离法进行分离纯化，观察形态学特征并结合ITS与β-tubulin序列进行鉴定。利用邻接法(Neighbor-joining, NJ)构建系统发育树，进行序列分析。结果表明代表菌株的菌落形态为白色、羽毛状，有气生菌丝产生，后期出现黑色产孢体。分生孢子器浅埋于树皮下，器壁黑褐色、内陷，孔口1个，分生孢子器由9至14个小腔室组成，共用璧，呈散射状分布，形态学特征均符合金黄壳囊孢(Cytospora chrysosperma)，ITS与β-tubulin序列与金黄壳囊孢(C. chrysosperma)同源性高达99%以上。通过致病性测定显示分离菌为胡杨的致病菌与新疆巴州胡杨腐烂病病原菌相同。

Abstract: Populus euphratica rot pathogen surrounding farmland shelterbelt was used as the research material in the Aksu region, and was isolated and purified by conventional tissue separation method. Morphological characteristics were observed and ITS and β-tubulin. The phylogenetic tree was constructed using Neighbor-joining (NJ) for sequence analysis. The results show that the colony morphology of the strain was white and feathery, with the generation of air-borne mycelium and the emergence of black sporogenesis in the later period. Conidia are shallowly buried under the bark, with conidium wall dark brown and sunken, and 1 orifice. Conidia are composed of 9 to 14 small cavities, sharing the same wall, with scatter-like distribution. Morphological characteristics are consistent with C. chrysosperma. Both are in line with C. chrysosperma. Biological analysis shows that the ITS and β-tubulin sequences are more than 99% homologous to C. chrysosperma. It is the same as the pathogen of Populus euphratica in Bazhou, and the pathogen of Populus euphratica.