LncRNA XIST、miR-204及FN1在甲状腺乳头状癌中的表达及意义

Expression and Significance of LncRNA XIST, miR-204 and FN1 in Papillary Thyroid Carcinoma

摘要: 目的：探讨LncRNA XIST，miR-204及FN1在PTC组织和癌旁组织及LncRNA XIST在PTC细胞系中的表达及意义。方法：应用Real-time PCR技术检测20例甲状腺乳头状癌和其癌旁组织中LncRNA XIST，miR-204及FN1的表达情况，应用QPCR检测3株甲状腺癌细胞系(BCPAP, TPC-1, KTC-1) LncRNA XIST、miR-204-5p的表达水平，应用荧光原位杂交(FISH)检测PTC细胞系BCPAP中LncRNA XIST的表达定位，应用双荧光素酶报告基因检测人293T细胞的LncRNA XIST、miR-204及FN1相互作用。结果：与癌旁组相比，PTC癌组织组的LncRNA XIST的表达水平显著上调(p < 0.05)，PTC癌组织组的FN1的表达水平极显著上调(p < 0.01)，而PTC癌组织组的miR-204-5p的表达水平极显著下调(p < 0.01)，差异均具有统计学意义；BCPAP细胞的LncRNA XIST的表达水平最高，且miR-204-5p的表达水平最低；LncRNA XIST主要在细胞质中表达；LncRNA XIST与miR-204有相互作用(p < 0.05)，FN1与miR-204有相互作用(p < 0.05)。结论：LncRNA XIST在甲状腺乳头状癌中高表达，miR-204在甲状腺乳头状癌中低表达，FN1在甲状腺乳头状癌中高表达；LncRNA XIST，miR-204及FN1相互作用，参与甲状腺乳头状癌的发生发展，并可为甲状腺乳头状癌潜在诊断标志物和治疗靶点的寻找提供理论依据，LncRNA XIST可能是甲状腺乳头状癌诊断和治疗的一个新靶点。

Abstract: Objective: To investigate the expression and significance of LncRNA XIST, miR-204 and FN1 in PTC tissues and adjacent tissues, and LncRNA XIST in PTC cell lines. Method: The expression of LncRNA XIST, miR-204 and FN1 in 20 cases of papillary thyroid carcinoma and its adjacent tissues were detected by Real-time PCR technology, and 3 thyroid cancer cell lines (BCPAP, TPC-1, KTC-1) were detected by QPCR, the expression levels of LncRNA XIST and miR-204-5p were detected by fluorescence in situ hybridization (FISH) to detect the expression and location of LncRNA XIST in the PTC cell line BCPAP, and double luciferase reporter gene was used to detect the interaction of LncRNA XIST, miR-204 and FN1 in human 293T cells. Result: The expression level of LncRNA XIST in the PTC cancer tissue group was significantly increased in comparison with the adjacent group (p < 0.05), the expression level of FN1 in the PTC cancer tissue group was extremely significantly up-regulated (p < 0.01), while the expression level of miR-204-5p in the PTC cancer tissue group was extremely significantly down-regulated (p < 0.01), the differences are statistically significant；the expression level of LncRNA XIST in BCPAP cells was the highest, and the expression level of miR-204-5p was the lowest; LncRNA XIST was mainly expressed in the cytoplasm; LncRNA XIST interacted with miR-204 (p < 0.05), and FN1 interacted with miR-204 (p < 0.05). Conclusion: LncRNA XIST is highly expressed in papillary thyroid carcinoma, miR-204 is low expressed in papillary thyroid carcinoma, and FN1 is highly expressed in papillary thyroid carcinoma; the interaction between LncRNA XIST, miR-204 and FN1 is involved in the occurrence and development of thyroid papillary carcinoma, and can provide a theoretical basis for the search of potential diagnostic markers and therapeutic targets for thyroid papillary carcinoma. LncRNA XIST may be a new target for the diagnosis and treatment of thyroid papillary carcinoma.