反硝化过程中的关键影响因素——电子供体
The Key Influencing Factor in Denitrification Process—Electron Donor
DOI: 10.12677/OJNS.2022.105101, PDF,  被引量    科研立项经费支持
作者: 范红叶, 郑燊鹏, 许宇虹, 刘皓月:浙江树人学院生物与环境工程学院,浙江 杭州;熊千慧:浙江树人学院树兰国际医学院,浙江 杭州;王泽宇*:浙江树人学院交叉科学研究院浙江省污染暴露与健康干预重点实验室,浙江 杭州
关键词: 反硝化电子供体有机碳源无机碳源Denitrification Electron Donor Organic Carbon Source Inorganic Carbon Source
摘要: 废水中的硝酸盐(NO3- )污染已成为一个严重的全球生态问题。生物脱氮通过反硝化作用将NO3-还原为氮气(N2),是一种高效、经济的去除NO3-的工艺。然而,NO3-作为电子受体须接受电子供体传递来的电子以完成其还原,因此电子供体的差异严重影响其反硝化性能。本文综述了用于生物脱氮的各种电子给体的最新进展。根据碳源(电子供体)的类型,反硝化可分为异养反硝化和自养反硝化。本综述从脱氮性能和环境影响方面,总结并比较了基于不同电子供体的生物脱氮工艺,并提出了探索异养/自养反硝化的最佳配置和不同的有机/无机源组合可能是未来的研究方向。
Abstract: Nitrate (NO3-) pollution in wastewater has become a serious global ecological problem. Biological denitrification reduces NO3- to nitrogen (N2) through denitrification, which is an efficient and economical process for removing NO3- . However, as an electron acceptor, NO3- must accept electrons from electron donors to complete its reduction, so the difference of electron donors seriously affects its denitrification performance. This article reviewed the recent progress of various electron donors for biological denitrification. According to the type of carbon source (electron donor), denitrification could be divided into heterotrophic denitrification and autotrophic denitrification. This review summarized and compared biological denitrification processes based on different electron donors in terms of denitrification performance and environmental impact. And it is proposed that explor-ing the optimal configuration of heterotrophic/autotrophic denitrification and different combinations of organic/inorganic sources may be future research directions.
文章引用:范红叶, 郑燊鹏, 许宇虹, 刘皓月, 熊千慧, 王泽宇. 反硝化过程中的关键影响因素——电子供体[J]. 自然科学, 2022, 10(5): 885-896. https://doi.org/10.12677/OJNS.2022.105101

参考文献

[1] Peng, C., et al. (2018) Identification of Nitrate Pollution Sources through Various Isotopic Methods: A Case Study of the Huixian Wetland. Environmental Science, 39, 5410-5417.
[2] Sheng, T., et al. (2018) Nitrate-Nitrogen Pollution Sources of an Underground River in Karst Agricultural Area Using 15N and 18O Isotope Technique. Environmental Sci-ence, 39, 4547-4555.
[3] Wang, Y.-L., Feng, M.-Q. and Dong, X.-Q. (2019) Analysis of Nitrate Pollution Sources in the Rainy Season of the Lower Fenhe River. Environmental Science, 40, 4033-4041.
[4] Chen, X., et al. (2022) High Efficient Bio-Denitrification of Nitrate Contaminated Water with Low Ammonium and Sulfate Production by a Sul-fur/Pyrite-Based Bioreactor. Bioresource Technology, 346, Article ID: 126669. [Google Scholar] [CrossRef] [PubMed]
[5] Graham, D.W., et al. (2010) Correlations between in Situ De-nitrification Activity and Nir-Gene Abundances in Pristine and Impacted Prairie Streams. Environmental Pollution (Barking, Essex: 1987), 158, 3225-3229. [Google Scholar] [CrossRef] [PubMed]
[6] Li, R., et al. (2016) Woodchip-Sulfur Based Heterotrophic and Autotrophic Denitrification (WSHAD) Process for Nitrate Contaminated Water Remediation. Water Research, 89, 171-179. [Google Scholar] [CrossRef] [PubMed]
[7] Tong, S., et al. (2013) Characteristics of Hetero-trophic/Biofilm-Electrode Autotrophic Denitrification for Nitrate Removal from Groundwater. Bioresource Technology, 148, 121-127. [Google Scholar] [CrossRef] [PubMed]
[8] Tian, T. and Yu, H.-Q. (2020) Denitrification with Non-Organic Electron Donor for Treating Low C/N Ratio Wastewaters. Bioresource Technology, 299, Article ID: 122686. [Google Scholar] [CrossRef] [PubMed]
[9] Liu, H., et al. (2022) Effect of S2O32−-S Addition on Anammox Coupling Sulfur Autotrophic Denitrification and Mechanism Analysis Using N and O Dual Isotope Effects. Water Research, 218, Article ID: 118404. [Google Scholar] [CrossRef] [PubMed]
[10] Yang, X., et al. (2022) Effect of Electric Current Intensity on Performance of Polycaprolactone/FeS2-Based Mixotrophic Biofilm-Electrode Reactor. Bioresource Technology, 361, Ar-ticle ID: 127757. [Google Scholar] [CrossRef] [PubMed]
[11] Bai, Y., et al. (2022) Role of Iron(II) Sulfide in Autotrophic Denitrification under Tetracycline Stress: Substrate and Detoxification Effect. The Science of the Total Environment, 850, Article ID: 158039. [Google Scholar] [CrossRef] [PubMed]
[12] Li, S., Jiang, Z. and Ji, G. (2022) Effect of Sulfur Sources on the Competition between Denitrification and DNRA. Environmental Pollution, 305, Article ID: 119322. [Google Scholar] [CrossRef] [PubMed]
[13] Pang, Y., Hu, L. and Wang, J. (2022) Mixotrophic Denitrifica-tion Using Pyrite and Biodegradable Polymer Composite as Electron Donors. Bioresource Technology, 351, Article ID: 127011. [Google Scholar] [CrossRef] [PubMed]
[14] Wang, P., et al. (2022) Use of Sponge Iron as an In-direct Electron Donor to Provide Ferrous Iron for Nitrate-Depen- dent Ferrous Oxidation Processes: Denitrification Per-formance and Mechanism. Bioresource Technology, 357, Article ID: 127318. [Google Scholar] [CrossRef] [PubMed]
[15] Zhang, H., et al. (2022) Nitrogen Removal from Low Car-bon/Nitrogen Polluted Water Is Enhanced by a Novel Synthetic Micro-Ecosystem under Aerobic Conditions: Novel In-sight into Abundance of Denitrification Genes and Community Interactions. Bioresource Technology, 351, Article ID: 127013. [Google Scholar] [CrossRef] [PubMed]
[16] Wang, J., et al. (2022) Elucidating the Role of Carbon Shell in Autotrophic Denitrification Driven by Carbon-Coated Nanoscale Zerovalent Iron. Chemical Engineering Journal, 434, Article ID: 134656. [Google Scholar] [CrossRef
[17] Zhang, X.-N., et al. (2022) Thiosulfate as External Electron Donor Accelerating Denitrification at Low Temperature Condition in S-0-Based Autotrophic Denitrification Biofilter. Environ-mental Research, 210, Article ID: 113009. [Google Scholar] [CrossRef] [PubMed]
[18] de Albuquerque, F.P., et al. (2022) Carbon Cloth Amendment for Boosting High-Solids Anaerobic Digestion with Percolate Recirculation: Spatial Patterns of Microbial Communities. Chemosphere, 307, Article ID: 135606. [Google Scholar] [CrossRef] [PubMed]
[19] Fu, X., et al. (2022) Application of External Carbon Source in Heterotrophic Denitrification of Domestic Sewage: A Review. Science of the Total Environment, 817, Article ID: 153061. [Google Scholar] [CrossRef] [PubMed]
[20] Li, C., et al. (2022) Initial Carbon Release Char-acteristics, Mechanisms and Denitrification Performance of a Novel Slow Release Carbon Source. Journal of Environ-mental Sciences, 118, 32-45. [Google Scholar] [CrossRef] [PubMed]
[21] Li, H., et al. (2022) Efficient Nitrogen Removal from Stormwater Runoff by Bioretention System: The Construction of Plant Carbon Source-Based Hetero-trophic and Sulfur Autotrophic Denitrification Process. Bioresource Technology, 349, Article ID: 126803. [Google Scholar] [CrossRef] [PubMed]
[22] Li, J., et al. (2006) Removal of Organic Matter and Nitrogen from Distillery Wastewater by a Combination of Methane Fermentation and Denitrification/Nitrification Processes. Jour-nal of Environmental Sciences (China), 18, 654-659.
[23] Louzeiro, N.R., et al. (2002) Methanol-Induced Biological Nutrient Removal Kinetics in a Full-Scale Sequencing Batch Reactor. Water Research, 36, 2721-2732. [Google Scholar] [CrossRef
[24] Xu, Z., Dai, X. and Chai, X. (2018) Effect of Different Car-bon Sources on Denitrification Performance, Microbial Community Structure and Denitrification Genes. Science of the Total Environment, 634, 195-204. [Google Scholar] [CrossRef] [PubMed]
[25] Gao, Y., et al. (2020) Denitrification Performance Evaluation and Kinetics Analysis with Mariculture Solid Wastes (MSW) Derived Carbon Source in Marine Recirculating Aquacul-ture Systems (RAS). Bioresource Technology, 313, Article ID: 123649. [Google Scholar] [CrossRef] [PubMed]
[26] Li, H., et al. (2021) Porous Solid Carbon Source-Supported Denitrification in Simulated Mariculture Wastewater. Environmental Technology, 42, 1196-1203. [Google Scholar] [CrossRef] [PubMed]
[27] Liu, F., et al. (2016) The Use of Fermentation Liquid of Wastewater Primary Sedimentation Sludge as Supplemental Carbon Source for Denitrification Based on Enhanced An-aerobic Fermentation. Bioresource Technology, 219, 6-13. [Google Scholar] [CrossRef] [PubMed]
[28] Si, Z., et al. (2018) Intensified Heterotrophic Denitrification in Constructed Wetlands Using Four Solid Carbon Sources: Denitrification Efficiency and Bacterial Community Structure. Bioresource Technology, 267, 416-425. [Google Scholar] [CrossRef] [PubMed]
[29] Igielski, S., Kjellerup, B.V. and Davis, A.P. (2019) Under-standing Urban Stormwater Denitrification in Bioretention Internal Water Storage Zones. Water Environment Research, 91, 32-44. [Google Scholar] [CrossRef
[30] Ghane, E., et al. (2018) Carbon Quality of Four-Year-Old Woodchips in a Denitrification Bed Treating Agricultural Drainage Water. Transactions of the Asabe, 61, 995-1000. [Google Scholar] [CrossRef
[31] Hellman, M., et al. (2021) Substrate Type Determines Micro-bial Activity and Community Composition in Bioreactors for Nitrate Removal by Denitrification at Low Temperature. Science of the Total Environment, 755, Article ID: 143023. [Google Scholar] [CrossRef] [PubMed]
[32] Fan, Z., Hu, J. and Wang, J. (2012) Biological Nitrate Re-moval Using Wheat Straw and PLA as Substrate. Environmental Technology, 33, 2369-2374. [Google Scholar] [CrossRef] [PubMed]
[33] Xu, Z., Dai, X. and Chai, X. (2019) Effect of Temperature on Tertiary Nitrogen Removal from Municipal Wastewater in a PHBV/PLA-Supported Denitrification System. Environ-mental Science and Pollution Research, 26, 26893-26899. [Google Scholar] [CrossRef] [PubMed]
[34] Chen, Y., et al. (2011) Effects of Dissolved Oxygen on Extra-cellular Enzymes Activities and Transformation of Carbon Sources from Plant Biomass: Implications for Denitrification in Constructed Wetlands. Bioresource Technology, 102, 2433-2440. [Google Scholar] [CrossRef] [PubMed]
[35] Yang, Z., et al. (2020) Nitrogen Removal Performance in Pi-lot-Scale Solid-Phase Denitrification Systems Using Novel Biodegradable Blends for Treatment of Waste Water Treat-ment Plants Effluent. Bioresource Technology, 305, Article ID: 122994. [Google Scholar] [CrossRef] [PubMed]
[36] Xie, Y., et al. (2019) An Iron-Carbon-Activated Carbon and Zeolite Composite Filter, Anaerobic-Aerobic Integrated Denitrification Device for Nitrogen Removal in Low C/N Ratio Sewage. Water Science and Technology, 80, 223-231. [Google Scholar] [CrossRef] [PubMed]
[37] Park, J.-H., et al. (2016) Pyrosequencing Analysis of Microbial Com-munities in Hollow Fiber-Membrane Biofilm Reactors System for Treating High-Strength Nitrogen Wastewater. Chem-osphere, 163, 192-201. [Google Scholar] [CrossRef] [PubMed]
[38] Zhu, C., et al. (2017) Enhanced Denitrification at Biocath-ode Facilitated with Biohydrogen Production in a Three- Chambered Bioelectrochemical System (BES) Reactor. Chemi-cal Engineering Journal, 312, 360-366. [Google Scholar] [CrossRef
[39] Mahl, U.H., et al. (2015) Two-Stage Ditch Floodplains Enhance N-Removal Capacity and Reduce Turbidity and Dissolved P in Agricultural Streams. Journal of the American Water Re-sources Association, 51, 923-940. [Google Scholar] [CrossRef
[40] Zhou, P., et al. (2021) Electrochemical Insight into the Activated Algal Biochar Assisted Hydrogenotrophic Denitrification at Biocathode Using Bioelectrochemical System (BES). Pro-cess Biochemistry, 103, 60-64. [Google Scholar] [CrossRef
[41] Fan, C., et al. (2021) Sulfur Transformation in Sulfur Auto-trophic Denitrification Using Thiosulfate as Electron Donor. Environmental Pollution, 268, Article ID: 115708. [Google Scholar] [CrossRef] [PubMed]
[42] Zhang, L., et al. (2021) Elemental Sulfur as Electron Donor and/or Acceptor: Mechanisms, Applications and Perspectives for Biological Water and Wastewater Treatment. Water Re-search, 202, Article ID: 117373. [Google Scholar] [CrossRef] [PubMed]
[43] Watsuntorn, W., et al. (2017) Hydrogen Sulfide Oxidation under Anoxic Conditions by a Nitrate-Reducing, Sulfide- Oxidizing Bacterium Isolated from the Mae Urn Long Luang Hot Spring, Thailand. International Biodeterioration & Biodegradation, 124, 196-205. [Google Scholar] [CrossRef
[44] Li, W., et al. (2013) Physical Characteristics and Formation Mechanism of Denitrifying Granular Sludge in High-Load Reactor. Bioresource Technology, 142, 683-687. [Google Scholar] [CrossRef] [PubMed]
[45] Yang, W., et al. (2016) Sulfide-Driven Autotrophic Denitrifica-tion Significantly Reduces N2O Emissions. Water Research, 90, 176-184. [Google Scholar] [CrossRef] [PubMed]
[46] Guo, J., et al. (2022) Selective Reduction of Nitrate to Nitrogen by Fe0-Cu0-CuFe2O4 Composite Coupled with Carbon Dioxide Anion Radical under UV Irradiation. Chemosphere, 295, Article ID: 133785. [Google Scholar] [CrossRef] [PubMed]
[47] Ruby, C., et al. (2006) In Situ Redox Flexibility of FeII-III Oxyhydroxycarbonate Green Rust and Fougerite. Environmental Science & Technology, 40, 4696-4702. [Google Scholar] [CrossRef] [PubMed]
[48] Meyer, A., et al. (2010) A New 5N Tracer Method to Determine N Turno-ver and Denitrification of Pseudomonas stutzeri. Isotopes in Environmental and Health Studies, 46, 409-421. [Google Scholar] [CrossRef] [PubMed]
[49] Mao, J., et al. (2022) Resource Utilization of Waste Tailings: Simulated Removal of Nitrogen from Secondary Effluent by Autotrophic Denitrification Based on Pyrite Tailings. Fron-tiers in Environmental Science, 10, Article ID: 949618. [Google Scholar] [CrossRef
[50] Li, R., et al. (2020) Pyrrhotite-Sulfur Autotrophic Denitrification for Deep and Efficient Nitrate and Phosphate Removal: Synergistic Effects, Secondary Minerals and Microbial Commu-nity Shifts. Bioresource Technology, 308, Article ID: 123302. [Google Scholar] [CrossRef] [PubMed]
[51] Yang, Y., et al. (2017) Nanostructured Pyrrhotite Supports Autotrophic Denitrification for Simultaneous Nitrogen and Phosphorus Removal from Secondary Effluents. Chemical Engineering Journal, 328, 511-518. [Google Scholar] [CrossRef
[52] Zhao, L., et al. (2022) Simultaneous Heterotrophic and FeS2-Based Ferrous Autotrophic Denitrification Process for Low-C/N Ratio Wastewater Treatment: Nitrate Removal Performance and Microbial Community Analysis. Science of the Total Environment, 829, Article ID: 154682. [Google Scholar] [CrossRef] [PubMed]
[53] Hosono, T., et al. (2015) Nitrogen, Carbon, and Sulfur Iso-topic Change during Heterotrophic (Pseudomonas aureofaciens) and Autotrophic (Thiobacillus denitrificans) Denitrifi-cation Reactions. Journal of Contaminant Hydrology, 183, 72-81. [Google Scholar] [CrossRef] [PubMed]
[54] Huang, Z., et al. (2020) Mercury Oxidation Coupled to Auto-trophic Denitrifying Branched Sulfur Oxidation and Sulfur Disproportionation for Simultaneous Removal of Hg(0) and NO. Applied Microbiology and Biotechnology, 104, 8489- 8504. [Google Scholar] [CrossRef] [PubMed]
[55] Soundaranayaki, K. and Gandhimathi, R. (2020) Enhancing the Nitrogen Removal of Vertical Flow Constructed Wetland by Using Organic Media. Desalination and Water Treatment, 175, 125-140. [Google Scholar] [CrossRef
[56] Sanchez, I., et al. (2008) Assessment of the Addition of Thiobacillus denitrificans and Thiomicrospira denitrificans to Chemolithoautotrophic Denitrifying Bioreactors. International Microbi-ology: The Official Journal of the Spanish Society for Microbiology, 11, 179-184.