PINK1和Parkin在结直肠癌组织中的表达及临床价值

Expression and Clinical Significance of PINK1 and Parkin in Colorectal Cancer

摘要: 目的：监测结直肠癌组织中与线粒体自噬过程紧密相关的基因PINK1和Parkin的表达情况，并探讨其存在的临床价值。方法：应用免疫组织化学染色SP法检测肠镜检查或手术过程中获取的120例结直肠癌组织、45例结直肠腺瘤组织和45例正常结直肠组织(距肿瘤 > 5 cm)中PINK1和Parkin的表达情况，分析PINK1和Parkin之间的内在关联性以及PINK1和Parkin的表达与结直肠癌患者的性别、年龄、肿瘤体积、病理类型、分化程度、淋巴结转移、远处转移和临床TNM分期等关联性。结果：免疫组化结果表明PINK1和Parkin阳性表达均主要发生在肿瘤细胞胞浆中。120例结直肠癌组织中PINK1和Parkin阳性表达率分别为56.67% (68/120)和47.5% (57/120)，明显低于肠腺瘤组织和正常肠组织中的表达(P < 0.01)。PINK1和Parkin蛋白在结直肠癌组织中的表达具有正相关性(r = 0.5528, P < 0.05)。在结直肠癌组织中，PINK1和Parkin的阳性表达与其分化程度、淋巴结转移、远处转移和临床TNM分期具有明显的关联性(P < 0.05)；而与患者年龄、性别、肿瘤体积、病理类型无关(P > 0.05)。结论：PINK1和Parkin在结直肠癌组织中呈现低水平表达状态，提示线粒体自噬活性的降低在结直肠癌的发生发展和侵袭转移中可能发挥了一定作用。

Abstract: Objective: To observe the expression of PINK1 and Parkin closely related to mitochondrial autoph-agy in colorectal cancerandn and to explore the clinical significance. Methods: Immunohistochemi-cal SP methods was applied to detect the expressions of PINK1 and Parkin in 120 colorectal cancer tissues, 45 intestinal adenoma tissues and 45 normal intestinal tissues (5 cm away from the cancer). The correlation between PINK1 and Parkin was analyzed; while, the relationships between the ex-pression of PINK1, Parkin and the clinicopathological features, including sex, age, size, pathological type, differentiation degress, lymph node metastasis, distant organs metastasis and TNM stage were analyzed. Results: Immunohistochemical results showed that the positive expressions of PINK1 and Parkin were mainly located in the cytoplasm of the cells, and their positive expression rates in 120 colorectal cancer tissues were 56.67% (68/120) and 47.5% (57/120), respectively, which were both significantly lower than those in intestinal adenoma tissues and normal intestinal tissues (P < 0.01). There was a positive correlation between the expression of PINK1 and Parkin in colorectal cancer (r = 0.5528, P < 0.05). In colorectal cancer, the positive expression of PINK1 and Parkin was significantly correlated with the degree of differentiation, distant organ metastasis, lymph node metastasis and TNM stage (P < 0.05); and they were both independent to age, sex, tumor size and pathological type (P > 0.05). Conclusion: PINK1 and Parkin were low expressed in colorectal cancer, which suggested that the decrease of the mitophagy activity may play a role in the occurrence, de-velopment, invasion and metastasis of colorectal cancer.