干旱胁迫下甘蓝型油菜叶片碳酸酐酶活性的变化
Changes of Carbonic Anhydrase Activities in Brassica napus Leaves under Drought Stress
DOI: 10.12677/BR.2024.132012, PDF,   
作者: 邓秋红, 罗丽丹, 张秀花, 陈舒丽*：湖北科技学院基础医学院，湖北 咸宁；罗刘明：宝武集团武汉钢铁有限公司，条材厂，湖北 武汉
关键词: 甘蓝型油菜；碳酸酐酶；干旱诱导；净光合速率；叶面喷锌；Brassica napus； Carbonic Anhydrase； Drought Stress； Net Photosynthesis Rate； Foliar Zinc Spraying
摘要: 本文研究了盆栽条件下叶面喷锌对抽薹期甘蓝型油菜叶片的碳酸酐酶活性和抗旱性的影响。用不同浓度的ZnSO4∙7H2O喷洒抽薹期油菜叶片1周后，进行干旱处理及复水处理。结果显示：在干旱渐进以及复水过程中喷锌组油菜叶片碳酸酐酶活性、净光合速率和脯氨酸含量均高于未喷锌组，而丙二醛含量则低于未喷锌组。说明叶面喷锌可以使甘蓝型油菜碳酸酐酶活性增加，进而可增加甘蓝型油菜的抗旱性。
Abstract: This paper studied the effects of foliar zinc spraying on the carbonic anhydrase activity and drought resistance of Brassica napus leaves during the bolting stage under potted conditions. The rapeseed leaves at the bolting stage were sprayed with different concentrations of ZnSO4∙7H2O for 1 week, followed by drought treatment and rehydration treatment. The results showed that the carbonic anhydrase activity, net photosynthetic rate and proline content of rapeseed leaves in the zinc-sprayed group were higher than those in the non-zinc-sprayed group, while the malondialde-hyde content was lower than that in the non-zinc-sprayed group during the gradual drought and rewatering process. This shows that foliar zinc spraying can increase carbonic anhydrase activities in Brassica napus, which in turn can increase the drought resistance of Brassica napus.
文章引用：邓秋红, 罗刘明, 罗丽丹, 张秀花, 陈舒丽. 干旱胁迫下甘蓝型油菜叶片碳酸酐酶活性的变化[J]. 植物学研究, 2024, 13(2): 103-109. https://doi.org/10.12677/BR.2024.132012

参考文献

[1] Tripp, B.C., Smith, K. and Ferry, J.G. (2001) Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Bi-ological Chemistry, 276, 48615-48618. [Google Scholar] [CrossRef] 
[2] Smith, K.S. and Ferry, J.G. (2000) Prokaryotic Carbonic Anhydrases. FEMS Microbiology Reviews, 24, 335-366. [Google Scholar] [CrossRef] [PubMed]
[3] 张震林, 高煜珠. 碳酸酐酶在高等植物光合碳代谢中的作用[J]. 江苏农业学报, 1992, 8(2): 7-12.
[4] Johansson, I.M. and Forsman, C. (1993) Kinetic Studies of Pea Carbonic Anhydrase. European Journal of Biochemistry, 218, 439-446. [Google Scholar] [CrossRef] [PubMed]
[5] Moskvin, O.V., Ivanov, B.N., Ignatova, L.K., et al. (2000) Light-Induced Stimulation of Carbonic Anhydrase Activity in Pea Thylakoids. FEBS Letters, 470, 375-377. [Google Scholar] [CrossRef] 
[6] Lu, Y.K. and Stemler, A.J. (2002) Extrinsic Photosystem II Carbonic Anhydrase in Maize Mesophyll Chloroplasts. Plant Physiology, 128, 643-649. [Google Scholar] [CrossRef] [PubMed]
[7] Rowlett, R.S., Chance, M.R., Wirt, M.D., et al. (1994) Kinetic and Struc-tural Characterization of Spinach Carbonic anhydrase. Biochemistry, 33, 13967-13976.
[8] Demir, N., Demir, Y. and Yildirim, A. (1997) Carbonic Anhydrases from Leaves and Roots of Daucus carota. Phytochemistry, 44, 1247-1250. [Google Scholar] [CrossRef] 
[9] 张悦扬, 赵希胜, 等. 烟草(Nicotiana tabacum)碳酸酐酶基因NtCA7的克隆及表达模式分析[J]. 分子植物育种, 2019, 17(14): 4567-4574.
[10] Hatch, M.D. and Burnell, J.N. (1990) Carbonic Anhydrase Activity in Leaves and Its Role in the First Step of C4 Photosynthesis. Plant Physiology, 93, 825-828. [Google Scholar] [CrossRef] [PubMed]
[11] Brownell, P.F., Bielig. L.M. and Grof, C.P.L. (1991) In-creased Carbonic Anhydrase Activity in Leaves of Sodium-Deficient C4 Plants. Australian Journal of Plant Physiology, 18, 589-592. [Google Scholar] [CrossRef] 
[12] 李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[13] Stewart, G.R. and Lee, J.A. (1974) The Role of Proline Accumulation in Halophytes. Planta, 120, 279-289. [Google Scholar] [CrossRef] 
[14] 武玉叶, 李德全. 土壤水分胁迫对冬小麦叶片渗透调节及叶绿体超微结构的影响[J]. 华北农学报, 2001, 16(2): 87-93.
[15] 姚庆群, 谢贵水. 干旱胁迫下光合作用的气孔与非气孔限制[J]. 热带农业科学, 2005, 25(4): 80-85.
[16] 孙卫红, 吴秋霞, 等. 干旱胁迫下番茄叶片碳酸酐酶活性的变化[J]. 植物生理学报, 2015, 51(4): 424-428.
[17] Wang, L.M., Jin, X., Li, Q.B., et al. (2016) Comparative Proteomics Reveals Phosphorylation of β Carbonic Anhydrase1 Might Be Important for Adaptation to Drought Stress in Brassica napus. Scientific Reports, 6, Article No. 39024. [Google Scholar] [CrossRef] [PubMed]