金边龙舌兰总皂苷的提取工艺及体外抗氧化研究

Study on Extraction Technology and in Vitro Antioxidant of Total Saponins from Agave americana var. Marginata

摘要: 目的：探究金边龙舌兰总皂苷提取率的影响因素，优选总皂苷的提取工艺条件，研究总皂苷的体外抗氧化活性。方法：通过改变乙醇浓度、料液比、超声时间、超声温度等参数，结合正交试验，来优化金边龙舌兰总皂苷的提取过程；利用对DPPH自由基和羟基自由基的清除率进行金边龙舌兰总皂苷的体外抗氧化研究。结果：提取工艺最佳条件为乙醇浓度60%，料液比1:25，超声时间60 min，超声温度80℃，提取率为10.213%。结论：正交试验优选了金边龙舌兰中总皂苷的最优提取过程，体外抗氧化研究验证了金边龙舌兰总皂苷具有一定的抗氧化性能。本研究为金边龙舌兰的进一步开发利用提供了依据。

Abstract: Objective: To explore the factors affecting the extraction rate of total saponins from Agave americana var. Marginata, optimize the extraction conditions of total saponins from Agave americana var. Marginata, and study the antioxidant activity of total saponins from Agave americana var. Marginata in vitro. Methods: By changing parameters such as ethanol concentration, solid-liquid ratio, ultrasound time, ultrasound temperature, and combining orthogonal experiments, the extraction process of total saponins from Agave americana var. Marginata was optimized; In vitro antioxidant study of total saponins from Agave americana var. Marginata using their scavenging rates against DPPH and hydroxyl radicals. Results: The optimum extraction conditions were as follows: the concentration of ethanol was 60%, the ratio of solid to liquid was 1:25, the ultrasonic time was 60 min, and the ultrasonic temperature was 80˚C. The extraction rate was 10.213%. Conclusion: The optimum extraction process of total saponins from Agave americana var. Marginata was optimized by orthogonal test, and the in vitro antioxidant study verified that the total saponins from Agave americana var. Marginata had good in vitro antioxidant activity. This study provides a basis for the further development and utilization of Agave americana var. Marginata.