烟草PILS6基因的生物信息学与表达模式分析

Bioinformatics Characteristics and Expression Pattern Analysis of PILS6 Gene in Nicotiana tabacum L.

摘要: 作为生长素输出载体的PILS6在生长素的极性运输中起关键作用，对植株根系的生长发育有重要影响。本研究以拟南芥AtPILS6基因为基础，通过生物信息学和表达模式分析，在烟草中鉴定4个NtPILS6基因(基因ID分别为Nta17g07840.1、Nta18g06260.1、Nta20g08460.1、Nta23g21260.1)。实验数据表明，这4个NtPILS6基因各自独立地分布在烟草的4条不同染色体上。进一步分析显示，这些基因所编码的NtPILS6蛋白具有相似的理化性质，氨基酸数量介于406至425个之间，分子量在44.31至46.30 kDa范围内，等电点值集中在8.92至9.06之间，且每个基因均含有10个内含子。亚细胞定位预测结果显示，NtPILS6蛋白定位于细胞质膜，并具备典型的跨膜结构。在启动子区域，存在多种与光、植物激素及环境胁迫响应相关的顺式作用元件。通过表达模式分析，发现NtPILS6基因在烟草的不同发育阶段和组织中均有表达，在根中表达显著，并且均在干旱胁迫下表达上调。研究结果将为更加深入地了解烟草NtPILS6基因及其在烟草根系生长发育过程中的功能验证奠定基础，为烤烟品种的遗传改良提供理论基础和基因资源。

Abstract: As an auxin efflux carrier, PILS6 plays a pivotal role in the polar transport of auxin, exerting substantial influence on the growth and development of plant root systems. Building upon the foundation of the Arabidopsis AtPILS6 gene, this study identified four NtPILS6 genes (with gene IDs Nta17g07840.1, Nta18g06260.1, Nta20g08460.1, and Nta23g21260.1) in tobacco through bioinformatics and expression pattern analysis. Experimental data revealed that these four NtPILS6 genes are independently distributed across four distinct chromosomes in tobacco. Further analysis indicated that the NtPILS6 proteins encoded by these genes share similar physicochemical properties, with amino acid counts ranging from 406 to 425, molecular weights spanning from 44.31 to 46.30 kDa, and isoelectric point values clustered between 8.92 and 9.06. Each gene contains 10 introns. Subcellular localization prediction results showed that NtPILS6 proteins are localized to the plasma membrane and possess typical transmembrane structures. In the promoter regions, a variety of cis-acting elements related to light, plant hormones, and environmental stress responses were identified. Expression pattern analysis demonstrated that NtPILS6 genes are expressed throughout different developmental stages and tissues in tobacco, with notable expression in roots, and all genes are upregulated under drought stress. These findings lay the groundwork for a deeper understanding of the tobacco NtPILS6 genes and the validation of its function in tobacco root growth and development, providing a theoretical basis and genetic resources for the genetic improvement of flue-cured tobacco varieties.