miR-34a-5p在糖尿病视网膜病玻璃体液中的表达及临床意义
The Clinical Significance of miR-34a-5p Level in Diabetic Retinopathy
DOI: 10.12677/jcpm.2024.34357, PDF,    科研立项经费支持
作者: 蒋晓云*, 鲍 宁, 蒋正轩, 陶黎明#：安徽医科大学第二附属医院眼科，安徽 合肥
关键词: miR-34a-5p；糖尿病视网膜病变；玻璃体液；生物标志物；Diabetic Retinopathy； miR-34a-5p； Vitreous Fluid； Biomarker
摘要: 目的：探究miR-34a-5p在糖尿病视网膜病患者玻璃体液中的表达水平及临床意义。方法：招募2023年10月至2024年2月安徽医科大学第二附属医院PDR患者共5例，特发性黄斑裂孔患者5例(对照组)。收集行玻璃体切除术的PDR患者玻璃体液5只眼，同期收取因特发性黄斑裂孔行玻璃体切除术患者玻璃体液5只眼为对照组，反转录实时荧光定量聚合酶连反应法(qRT-PCR)检测玻璃体液miR-34a-5p的表达。所有统计学均用SPSS 26.0进行分析，采用GraphPad Prism 8软件进行作图。结果：miR-34a-5p在糖尿病视网膜病变患者玻璃体液中的表达上升。结论：miR-34a-5p可作为糖尿病视网膜病新型生物标志物和治疗靶标。
Abstract: Aim: To investigate the expression of miR-34a-5p in vitreous fluid in human body, and explore the clinical significance of miR-34a-5p in diabetic retinopathy. Methods: A total of 5 patients with PDR and 5 patients with idiopathic macular hole (IMH) were recruited from the Second Affiliated Hospital of Anhui Medical University from October 2023 to February 2024. Vitreous fluid was collected from 5 eyes of PDR patients before vitrectomy, and another vitreous fluid from 5 eyes of IMH patients collected as the control group. The levels of miR-34a-5p were detected by qRT-PCR. Basic information and samples of vitreous fluid were collected before vitrectomy. All statistics were analyzed using SPSS 26.0, and GraphPad Prism 8 software was used for mapping. Results: The expression of miR-34a-5p increased in vitreous fluid of diabetic retinopathy patients. Conclusions: MiR-34a-5p can be used as a novel biomarker and therapeutic target for diabetic retinopathy.
文章引用：蒋晓云, 鲍宁, 蒋正轩, 陶黎明. miR-34a-5p在糖尿病视网膜病玻璃体液中的表达及临床意义[J]. 临床个性化医学, 2024, 3(4): 2505-2511. https://doi.org/10.12677/jcpm.2024.34357

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