不同黑种草子挥发油中百里醌的含量

Content of Thymoquinone in Volatile Oil from Black Seed (Nigella) of Different Habitats

摘要: 目的：测定不同产地黑种草子挥发油中百里醌的含量。方法：将5种不同产地的黑种草子用两种不同化学提取方法提取挥发油，建立定量核磁共振波谱法测定挥发油中的百里醌。使用500 MHZ Bruker Avance III (TMS, δ = 0 ppm)核磁共振谱仪，以氖代氯仿为溶剂，在测定温度25℃、延迟时间2 s和扫描次数64的条件下扫描，通过化学移位及峰面积归一化法测定各黑种草子挥发油中百里醌相对百分比含量。结果：以化学位移在δ = 6.5 ppm与δ = 6.6 ppm处的百里醌的氢质子峰作为定量峰，其峰面积比与其质量比的线性回归方程为Y = 0.493x − 0.0005，相关系数为0.999。5种不同产地黑种草子挥发油中百里醌在方法1中测得USA1 (0.065%)、USA2 (0.093%)、USA3 (0.043%)、Tibe (0.043%)、Guangxi (0.062%)。在方法2中测得USA1 (0.263%)、USA2 (0.153%)、USA3 (0.037%)、Tibet (0.200%)、Guangxi (0.224%)。结论：分析结果表明，因产地及提取方法差异，黑种草子挥发油中百里醌含量测定是有差异的。文章通过测定不同产地黑种草子挥发油中的百里醌含量，为进一步研究开发黑种草子的药用价值奠定基础。

Abstract: Objective: To determine the content of quinone in volatile oil from black seed (Nigella) of different habitats. Methods: The essential oils were extracted from the seeds of black grass from five different habitats by two different chemical extraction methods, and the thymoquinone in the essential oils was determined by quantitative nuclear magnetic resonance spectroscopy. A 500 MHz Bruker Avance III (TMS, δ = 0 ppm) nuclear magnetic resonance spectrometer was used, with deuterochloroform as the solvent, at a measurement temperature of 25˚C, a delay time of 2 seconds, and 64 scans, to determine the relative percentage content of thymoquinone in the volatile oils of black grass seeds by chemical shift and peak area normalization. Results: Using the hydrogen proton peak of thymoquinone with a chemical shift at δ = 6.5 ppm and δ = 6.6 ppm as the quantitative peak, the peak area ratio of thymoquinone and its mass ratio were linear regression equation Y = 0.493x − 0.0005, the correlation coefficient was 0.999, and thymoquinone in volatile oils of black grass from five different habitats was determined as USA1 (0.065%), USA2 (0.093%), USA3 (0.043%), Tibet (0.043%), Guangxi (0.062%) in method 1. In method 2, USA1 (0.263%), USA2 (0.153%), USA3 (0.037%), Tibet (0.200%), and Guangxi (0.224%) were measured. Conclusion: The results showed that there were differences in the determination of thymoquinone content in the essential oil of Herba Nigella due to the differences in origin and extraction methods. In this paper, the content of thymoquinone in the essential oil of black seed grass from different habitats was determined to lay a foundation for further research and development of the medicinal value of black seed grass.