低氧胁迫下红鳍东方鲀肝脏全基因组甲基化和基因表达分析
Genome-Wide Methylation and Gene Expression Analysis of Liver of Takifugu Rubripes under Hypoxic Stress
DOI: 10.12677/ojfr.2025.121005, PDF,    科研立项经费支持
作者: 向雪建, 吴思润, 魏仁杰, 李 岩, 陆 芸, 刘 洋*：大连海洋大学水产与生命学院，辽宁 大连；王秀利：大连海洋大学水产与生命学院，辽宁 大连；辽宁省河鲀良种繁育及健康养殖重点实验室，辽宁 大连
关键词: 红鳍东方鲀；低氧；肝脏；甲基化；转录组；Takifugu rubripes； Hypoxia； Liver； Methylation； Transcriptome
摘要: 本研究利用WGBS和RNA-Seq技术，比较分析了常氧和低氧胁迫下红鳍东方鲀肝脏的DNA甲基化和基因表达差异。研究鉴定了差异甲基化区域(DMRs)、差异甲基化基因(DMGs)和差异表达基因(DEGs)，并进行了GO和KEGG富集分析。通过关联甲基化和转录组数据，探索了二者之间的潜在联系，发现甲基化程度升高与转录组表达降低呈负相关趋势，并鉴定了一些在甲基化和转录组数据中同时富集的通路和基因，例如robo2和prkcb。研究旨在揭示低氧胁迫下红鳍东方鲀肝脏的分子机制。
Abstract: In this study, WGBS and RNA-Seq technology were used to compare and analyze the differences in DNA methylation and gene expression in the liver of Takifugu rubripes under normoxia and hypoxic stress. Differentially methylated regions (DMRs), differentially methylated genes (DMGs) and differentially expressed genes (DEGs) were identified and GO and KEGG enrichment analyses were performed. By correlating methylation and transcriptome data, the potential link between the two was explored, and a negative correlation between increased methylation and decreased transcriptome expression was found, and some pathways and genes enriched in both methylation and transcriptome data were identified, such as robo2 and prkcb. The aim of this study was to reveal the molecular mechanism of the liver of the Takifugu rubripes under hypoxic stress.
文章引用：向雪建, 吴思润, 魏仁杰, 李岩, 陆芸, 刘洋, 王秀利. 低氧胁迫下红鳍东方鲀肝脏全基因组甲基化和基因表达分析 [J]. 水产研究, 2025, 12(1): 33-49. https://doi.org/10.12677/ojfr.2025.121005

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