基于网络药理学的刺五加注射液治疗冠心病心绞痛作用机制

Mechanism of Action of Eleuthero in the Treatment of Angina Pectoris in Coronary Heart Disease Based on Network Pharmacology

摘要: 目的：初步探索刺五加注射液治疗冠心病心绞痛的作用机制。方法：通过检索ECTM数据库和文献收集刺五加注射液的化合物成分，后利用PubChem、SwissADME、SwissTargetPrediction数据库获得刺五加注射液的化合物靶点数据集。通过TTD、DisGeNET、GeneCards三大数据库获得冠心病心绞痛相关靶点，与刺五加注射液靶点合并去重后取交集并绘制Venny图。运用Cyoscape 3.9.1软件筛选核心靶点与构建疾病–交集靶点–化合物网络。利用Metascape平台进行KEGG通路和GO功能富集分析，并利用微生信平台绘制点线图和矩阵点图。利用Autodock tools1.5.7、Autodock vina1.1.2对核心靶点及对应成分进行分子对接，并运用Pymol 2.5软件进行视化处理。结果：刺五加注射液主要作用于TNF、IL6、NFKB1、HIF-1A等核心靶点，调节IL-17、脂质与动脉粥样硬化等信号通路从而发挥治疗冠心病心绞痛的作用。

Abstract: Objective: To explore the mechanism of action of Eleuthero in the treatment of coronary heart disease angina. Methods: The compound components of Eleuthero were collected by searching the ECTM database and literature, and then the compound target dataset of Eleuthero was obtained by using PubChem, SwissADME and SwissTargetPrediction databases. The angina related targets of coronary heart disease were obtained through the three databases of TTD, DisGeNET and GeneCards, and the targets of Eleuthero were combined and deduplicated, and the Venny diagram was plotted. Cyoscape 3.9.1 software was used to screen core targets and construct disease-intersection target-compound networks. The Metascape platform was used for KEGG pathway and GO function enrichment analysis, and the micro-biotech platform was used to draw dot line diagrams and matrix dot diagrams. Autodock tools 1.5.7 and Autodock vina 1.1.2 were used to molecularly dock the core targets and corresponding components, and Pymol 2.5 software was used for visualization processing. Results: Eleuthero mainly acts on core targets such as TNF, IL6, NFKB1, HIF-1A, etc., and regulates IL-17, lipid and atherosclerosis signaling pathways, thereby playing a role in the treatment of coronary heart disease angina.