“道家补益禁方”对小鼠非特异性免疫功能的影响

The Effect of “The Tonifying Secret Recipe of Taoists” on Non-Specific Immune Function in Mice

摘要: 目的：探讨“道家补益禁方”对小鼠非特异性免疫功能的影响，为其开发利用奠定基础。方法：采用MTT法、溶血分光光度法、改良Mayer法及抗体芯片法检测小鼠非特异性免疫功能及细胞因子含量。结果：应用“道家补益禁方”后，小鼠的NK细胞杀伤力、CH50活性均增加；IL 2含量显著提高，IL 8分泌减少。结论：“道家补益禁方”能提高小鼠的非特异性免疫应答水平，调节细胞因子分泌，可以有效地增强机体非特异性免疫功能。 Objective: To explore the effect of “The tonifying secret recipe of Taoists” on non-specific immune function in mice, to establish the foundation for its development and utilization. Methods: To assay the immunity function and cytokine of mice with MTT, hemolysis spectrophotometric method, improved Mayer method and ELISA method. Results: “The tonifying secret recipe of Taoists” improved activity of NK and the total complement activity in serum, while increasing content of IL 2 and reduced that of IL 8. Conclusion: “The tonifying secret recipe of Taoists” could reinforce non-specific immune response, accommodate lymphocyte to secrete cytokine and enhance the body’s non-specific immune function effectively.