乙肝病毒生物标志物及其检测方法研究进展
Progress of Hepatitis B Virus Biomarkers and Detection Methods
DOI: 10.12677/MD.2013.34013, PDF, HTML,    科研立项经费支持
作者: 张力玲：广州计量检测技术研究院，广州；隋志伟, 刘瑛颖, 王 晶, 傅博强：中国计量科学研究院，北京；陈 文, 王佳媛：北京联合大学应用文理学院，北京
关键词: 乙型肝炎病毒；生物标志物；检测；Hepatitis B Virus; Biomarker; Detection
摘要: 全世界约有3.5亿人感染乙型肝炎病毒(Hepatitis B virus, HBV)，其中中国有约1.2亿人。应用HBV生物标志物检测，对于HBV的诊断、防治至关重要。目前我国针对HBV的检测方法非常多，这些检测方法基于不同的HBV生物标志物和不同的检测原理，其在临床上的应用以及检测效果也有所不同，因此了解HBV生物标志物及其检测方法的最新进展就显得更为重要。本文逐一介绍了HBV的理化特征、结构、生物标志物以及检测方法的研究进展，以供HBV的临床检测参考。 Hepatitis B is one of the most common infectious diseases around the world. About 350 million people are infected with hepatitis B virus totally in the world, and 120 million people are infected in China. It is very important to apply biomarker detection for diagnosis, prevention and treatment of HBV. At present, there are many detection methods of HBV based on different biomarkers and principles, and they have different clinic application and detection effects, so the progress of the HBV biomarkers and detection methods is more and more important. This article introduced the physical and chemical characteristics, structure, biomarkers of HBV and the research progress of analytical methods, in order to provide the reference for the clinical detection of HBV.
文章引用：张力玲, 隋志伟, 刘瑛颖, 王晶, 傅博强, 陈文, 王佳媛. 乙肝病毒生物标志物及其检测方法研究进展[J]. 医学诊断, 2013, 3(4): 57-63. http://dx.doi.org/10.12677/MD.2013.34013

参考文献

[1] 吴簧, 沈佐君 (2010) 乙型肝炎病毒基因型的中国国内研究进展. 国际检验医学杂志, 7, 703-704.
[2] 梅玉峰, 黄敏, 陈丽娟 (2010) HBV-DNA 阳性乙肝感染者血清学标志物临床分析. 国际检验医学杂志, 9, 1004-1005.
[3] 彭文伟 (1997) 病毒性肝炎研究. 广东科技出版社, 广州, 1.
[4] 田华, 王淑琴, 高建英 (2001) FQ-PCR 检测乙型肝炎患者血清HBV-DNA. 上海医学检验杂志, 6, 363.
[5] 高寿征 (1993) 病毒性肝炎. 人民军医出版社, 北京, 1.
[6] Lok, A.S.F. (2000) Hepatitis B infection: Pathogenesis and management．Journal of Hepatology, 32, 89.
[7] 何长伦, 许家璋, 畅志国等 (1998) 单一庚型肝炎病毒感染的临床和病理. 南京部队医药, 4, 8-10.
[8] 成萍, 尤忠胜, 孔玉英等 (1999) 乙型肝炎病毒的核心启动子各区段功能的研究. 生物化学与生物物理学报, 5, 577.
[9] Reifenberg, K., Lohler, J., Pudollek, H.P., et al. (1997) Long- term expression of the hepatitis B virus core-e-and x protein does not cause pathologic changes in transgenic mice. Journal of Hepatology, 26, 119.
[10] Wang, X.W., Gibson, M.K., Vermulen, W., et a1. (1995) Abrogation of p53-induced apoptosis by the hepatitis B virus X gene. Cancer Research, 23, 53.
[11] Prince, P., Hirschman, S.Z. and Garfinkel, E. (1980) DNA cloned from the ayw subtype of hepatitis B virus. Journal of Me- dical Virology, 6, 139-145.
[12] Chemin, I., Baginski, I., Petit, M.A., et al. (1991) Correlation between HBV-DNA detection by polymerase chain reaction and Pre-S1 antigenemia in symptomatic and asymptomatic hepatitis B virus infections. Journal of Medical Virology, 33, 51-57.
[13] 李琴, 孙桂珍, 魏玉香等 (2004) Pre-S1蛋白与乙肝病毒DNA 和e抗原在诊断乙肝病毒复制时的对比性研究. 中华肝脏病杂志, 12, 134-136.
[14] Bonino, F. and Brunetto M.R. (1993) Hepatitis B virus heterogeneity, one of many factors influencing the severity of hepatitis B. Journal of Hepatology, 18, 5-8．
[15] Suzuki, F., Miyakoshi, H., Kobayashi, M., et a1. (2009) Correla- tion between serum hepatitis B virus core-related antigen and intrahepatie covalently closed circular DNA in chronic hepatitis B patients. Journal of Medical Virology, 81, 27-33．
[16] Tanaka, E., Matsumoto, A. and Yoshizawa, K. (2008) Hepatitis B core-related antigen assay is useful for monitoring the antiviral effects of nucleoside analogue therapy. Inter virol. , l, 3-6．
[17] Guo, H.T., Jiang, D., Zhou, T.L., et a1. (2007) Characterization of the intracellular deproteinized relaxed circular DNA of hepatitis B virus: An intermediate of covalently closed circular DNA formation. Journal of Virology, 81, 12472-12484．
[18] 吴凤婷, 吕其军 (2007) 肝病患者血清中HBVcccDNA检测的临床意义. 肝脏, 4, 246-248．
[19] Pollicino, T., Squadrito, G., Cerenzir, G., et al. (2004) Hepatitis B virus maintains its pro-oncogenic properties in the case of occult HBV infection. Gastroenterology, 126, 102-110.
[20] Yuen, M.F., Wong, D.K., Sablon, E., et al. (2004) HBsAg seroclearance in chronic hepatitis B in the Chinese: Virological, histological, and clinical aspects. Hepatology, 39, 1694-1701.
[21] Werle-Lapostolle, B., Bowden, S., Locarnini, S., et al. (2004) Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy. Gastroenterology, 126, 1750-1758.
[22] Yuen, M.F., Wong, D.K., Fung, J., et al. (2008) HBsAg seroclearance in chronic hepatitis B in Asian patients: Replicative level and risk of hepatocellular carcinoma. Gastroenterology, 135, 1192-1199.
[23] Chen, Y., Sze, J. and He, M.L. (2004) HBV cccDNA in patients’ sera as an indicator for HBV reactivation and an early signal of liver damage. World Journal of Gastroenterology, 10, 82-85.
[24] Lu, L., Zhang, Y.H., Yueng, Y.H., et al. (2009) Intracellullar levels of hepatitis B virus DNA and pregenomic RNA in peripheral blood mononuclear cells of chronically infected patients. Journal of Viral Hepatitis, 16, 104-112.
[25] Takkenberg, R.B.，Zaaijer, H.L., Molenkamp, R., et al. (2009) Validation of a sensitive and specific real-time PCR for detection and quantitation of hepatitis B virus covalently closed circular DNA in plasma of chronic hepatitis B patients. Journal of Medical Virology, 81, 988-995.
[26] Faria, L.C., ,Gigou, M., Roque-Afonso, A.M., et al. (2008) He- patocellular carcinoma is associated with an increased risk of hepatitis B virus recurrence after liver transplantation. Gastro- enterology, 134, 1890-1899.
[27] Lenci, I., Marcuccilli, F., Tisone, G., et al. (2010) Total and covalently closed circular DNA detection in liver tissue of long- term survivors transplanted for HBV-related cirrhosis. Digestive and Liver Disease, 42, 578-584.
[28] 中华医学会肝病分会, 中华医学会感染病学分会 (2006) 慢性乙型肝炎防治指南. 中国病原生物学杂志, l, 67-76.