单细胞RNA测序在瘢痕疙瘩研究中的 应用进展
Advances in the Application of Single-Cell RNA Sequencing in Keloid Research
DOI: 10.12677/acm.2026.163868, PDF,   
作者: 袁 鑫, 涂一捷*, 王钲娟：赣南医科大学第一临床医学院，江西 赣州；谢林海#：赣南医科大学第一附属医院整形外科，江西 赣州
关键词: 瘢痕疙瘩；单细胞RNA测序；细胞异质性；分子机制；精准医疗；Keloid； Single-Cell RNA Sequencing； Cellular Heterogeneity； Molecular Mechanism； Precision Therapy
摘要: 瘢痕疙瘩是一种皮肤纤维增生性疾病，表现为伤口愈合过程中组织的过度修复和侵袭性生长，其临床治疗复发率高且缺乏特异性根治手段。由于瘢痕疙瘩组织内部存在高度的细胞异质性以及复杂的分子调控网络，传统研究技术难以全面解析其微环境全景。单细胞RNA测序(scRNA-seq)技术通过在单细胞分辨率的视角下获取转录组信息，重塑了人们对该疾病的认知。本文综述了scRNA-seq在瘢痕疙瘩研究中的最新应用进展，重点包括利用该技术绘制瘢痕疙瘩细胞图谱和解析瘢痕疙瘩的复杂分子机制。同时，本文也探讨了基于scRNA-seq发现的分子分型系统、潜在生物标志物及治疗靶点，并展望了scRNA-seq在临床转化中的前景与挑战，旨在为瘢痕疙瘩的精准诊疗提供理论依据。
Abstract: Keloids are a dermal fibroproliferative disorder characterized by excessive tissue repair and invasive growth during wound healing, with high clinical recurrence rates and a lack of specific radical treatments. Due to the high cellular heterogeneity and complex molecular regulatory networks within keloid tissue, conventional research techniques have been unable to comprehensively dissect its microenvironmental landscape. Single-cell RNA sequencing (scRNA-seq), by providing transcriptomic information at single-cell resolution, has reshaped our understanding of this disease. This review summarizes recent advances in the application of scRNA-seq in keloid research, focusing on its use in mapping the cellular atlas of keloids and deciphering the intricate molecular mechanisms within keloid tissue. Furthermore, it discusses molecular subtyping systems, potential biomarkers, and therapeutic targets identified based on scRNA-seq findings. The prospects and challenges of translating scRNA-seq discoveries into clinical practice are also explored, aiming to provide a theoretical foundation for the precision medicine of keloids.
文章引用：袁鑫, 涂一捷, 王钲娟, 谢林海. 单细胞RNA测序在瘢痕疙瘩研究中的 应用进展[J]. 临床医学进展, 2026, 16(3): 954-963. https://doi.org/10.12677/acm.2026.163868

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