黄精多糖提取工艺优化及抗氧化与降血糖活性研究
Optimization of Polysaccharide Extraction Process from Polygonatum sibiricum and Study on Its Antioxidant and Hypoglycemic Activities
DOI: 10.12677/br.2026.152012, PDF,    科研立项经费支持
作者: 蒋丝丝, 马 敏*, 董 丹：青海民族大学药学院，青海 西宁；叶 菊, 李庆群：遵义医药高等专科学校，药学院，贵州 遵义
关键词: 黄精多糖；响应面法；抗氧化活性；降血糖活性；Polygonatum sibiricum Polysaccharide； Response Surface Methodology； Antioxidant Activity； Hypoglycemic Activity
摘要: 目的：优化黄精多糖提取工艺及评价其抗氧化和降血糖活性。方法：以黄精多糖提取率为指标，考察粉碎粒度、提取时间、pH值、提取次数对热回流法提取效果的影响，采用Box-Behnken响应面法优化多糖的最佳提取工艺，并测定该条件下黄精多糖的抗氧化和降血糖活性。结果：黄精多糖的热回流法最佳提取工艺为粉碎粒度120目、提取时间2 h/次、提取次数2次、pH 8、水浴温度80℃，该工艺条件下总多糖的提取率达到(13.08 ± 0.42)%。当黄精多糖质量浓度为1.0 mg/mL时，其对ABTS+自由基的清除率达到 86.6%，对α-淀粉酶的抑制率达到56.8%。结论：最佳提取工艺条件为提高黄精多糖提取率以及开发具有抗氧化、降血糖产品提供了可靠的研究依据。
Abstract: Objective: To optimize the extraction process of polysaccharides from Polygonatum sibiricum and evaluate their antioxidant and hypoglycemic activities. Methods: Taking the extraction rate of polysaccharides from Polygonatum sibiricum as the index, the effects of particle size, extraction time, pH value and extraction times on the extraction efficiency by hot reflux method were investigated. The optimal extraction process of polysaccharides was optimized by Box-Behnken response surface methodology, and the antioxidant and hypoglycemic activities of polysaccharides under this condition were determined. Results: The optimal extraction process of polysaccharides from Polygonatum sibiricum by hot reflux method is as follows: particle size of 120 mesh, extraction time of 2 hours per time, extraction times of 2, pH of 8, and water bath temperature of 80˚C. Under these conditions, the extraction rate of total polysaccharides reaches (13.08 ± 0.42)%. When the mass concentration of polysaccharides from Polygonatum sibiricum is 1.0 mg/mL, the scavenging rate of ABTS+ free radicals reaches 86.6%, and the inhibitory rate of α-amylase reaches 56.8%. Conclusion: The process conditions provide a reliable research basis for improving the extraction rate of polysaccharides from Polygonatum sibiricum and developing products with antioxidant and hypoglycemic activities.
文章引用：蒋丝丝, 马敏, 叶菊, 董丹, 李庆群. 黄精多糖提取工艺优化及抗氧化与降血糖活性研究[J]. 植物学研究, 2026, 15(2): 96-108. https://doi.org/10.12677/br.2026.152012

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