海洋微塑料的分析技术研究进展
Research Progress in Analytical Techniques for Marine Microplastics
摘要: 海洋塑料污染已成为全球关注的重大环境问题,其衍生的微塑料因粒径微小、分布广泛、易被生物摄食等特性,对海洋生态系统乃至人类健康构成潜在威胁。当前微塑料污染日益严峻,微塑料在环境中的迁移、转化、归趋和生态毒理效应研究亟需深化,而建立标准化、高效的分析方法既是环境监测的基础,也是开展上述研究的重要前提。本文系统综述了当前国内外海水样品微塑料的采集、预处理及定性、定量检测技术,梳理了人工智能(AI)、高光谱成像技术(HSI)等新型检测技术的应用进展,对比了各类方法的优势与局限,针对现有技术短板明确了未来发展方向。本文对现有技术的系统整合,尤其是传统方法与新兴技术的融合尝试,既反映了跨学科融合的发展趋势,也为后续相关研究提供了参考,对初涉该领域的研究人员具有一定的参考价值。
Abstract: Marine plastic pollution has become a major global environmental concern, and microplastics derived from it pose potential threats to marine ecosystems and even human health due to their characteristics of small particle size, wide distribution, and high susceptibility to ingestion by organisms. As microplastic pollution grows increasingly severe, research on the migration, transformation, fate, and ecotoxicological effects of microplastics in the environment urgently requires further advancement, and establishing standardized and efficient analytical methods is not only the foundation of environmental monitoring but also an essential prerequisite for conducting such research. This paper systematically reviews current technologies for the collection, pretreatment, qualitative, and quantitative detection of microplastics in seawater samples worldwide, summarizes the application progress of emerging detection technologies such as artificial intelligence (AI) and hyperspectral imaging (HSI), compares the advantages and limitations of various methods, and clarifies future development directions to address the shortcomings of existing technologies. The systematic integration of existing technologies in this paper—particularly the attempt to integrate traditional methods with emerging ones—not only reflects the trend of interdisciplinary integration but also provides a valuable reference for subsequent related studies, offering practical guidance and theoretical support to researchers who are new to this field and promoting the in-depth development of microplastic detection and environmental monitoring research.
文章引用:蔡明桐. 海洋微塑料的分析技术研究进展[J]. 环境保护前沿, 2026, 16(3): 430-443. https://doi.org/10.12677/aep.2026.163043

参考文献

[1] Nizzetto, L., Bussi, G., Futter, M.N., Butterfield, D. and Whitehead, P.G. (2016) A Theoretical Assessment of Microplastic Transport in River Catchments and Their Retention by Soils and River Sediments. Environmental Science: Processes & Impacts, 18, 1050-1059. [Google Scholar] [CrossRef] [PubMed]
[2] Jambeck, J.R., Geyer, R., Wilcox, C., Siegler, T.R., Perryman, M., Andrady, A., et al. (2015) Plastic Waste Inputs from Land into the Ocean. Science, 347, 768-771. [Google Scholar] [CrossRef] [PubMed]
[3] Carpenter, E.J., Anderson, S.J., Harvey, G.R., Miklas, H.P. and Peck, B.B. (1972) Polystyrene Spherules in Coastal Waters. Science, 178, 749-750. [Google Scholar] [CrossRef] [PubMed]
[4] Wang, J., Peng, J., Tan, Z., Gao, Y., Zhan, Z., Chen, Q., et al. (2017) Microplastics in the Surface Sediments from the Beijiang River Littoral Zone: Composition, Abundance, Surface Textures and Interaction with Heavy Metals. Chemosphere, 171, 248-258. [Google Scholar] [CrossRef] [PubMed]
[5] Lee, J., Hong, S., Song, Y.K., Hong, S.H., Jang, Y.C., Jang, M., et al. (2013) Relationships among the Abundances of Plastic Debris in Different Size Classes on Beaches in South Korea. Marine Pollution Bulletin, 77, 349-354. [Google Scholar] [CrossRef] [PubMed]
[6] Schwabl, P., Köppel, S., Königshofer, P., Bucsics, T., Trauner, M., Reiberger, T., et al. (2019) Detection of Various Microplastics in Human Stool: A Prospective Case Series. Annals of Internal Medicine, 171, 453-457. [Google Scholar] [CrossRef] [PubMed]
[7] Leslie, H.A., van Velzen, M.J.M., Brandsma, S.H., Vethaak, A.D., Garcia-Vallejo, J.J. and Lamoree, M.H. (2022) Discovery and Quantification of Plastic Particle Pollution in Human Blood. Environment International, 163, Article 107199. [Google Scholar] [CrossRef] [PubMed]
[8] Kanhai, L.D.K., Officer, R., Lyashevska, O., Thompson, R.C. and O’Connor, I. (2017) Microplastic Abundance, Distribution and Composition along a Latitudinal Gradient in the Atlantic Ocean. Marine Pollution Bulletin, 115, 307-314. [Google Scholar] [CrossRef] [PubMed]
[9] Garini, Y., Young, I.T. and McNamara, G. (2006) Spectral Imaging: Principles and Applications. Cytometry Part A, 69, 735-747. [Google Scholar] [CrossRef] [PubMed]
[10] Lin, C., Huang, S., Lin, T. and Wu, P.C. (2023) Metasurface-Empowered Snapshot Hyperspectral Imaging with Convex/Deep (CODE) Small-Data Learning Theory. Nature Communications, 14, Article No. 6979. [Google Scholar] [CrossRef] [PubMed]
[11] Cozzolino, D., Williams, P.J. and Hoffman, L.C. (2023) An Overview of Pre-Processing Methods Available for Hyperspectral Imaging Applications. Microchemical Journal, 193, Article 109129. [Google Scholar] [CrossRef
[12] Amigo, J.M., Babamoradi, H. and Elcoroaristizabal, S. (2015) Hyperspectral Image Analysis. a Tutorial. Analytica Chimica Acta, 896, 34-51. [Google Scholar] [CrossRef] [PubMed]
[13] 薛庆生, 白皓轩, 李辉, 等. 水下高光谱成像探测技术研究进展(特邀) [J]. 光子学报, 2021, 50(12): 1-26.
[14] Jiang, H., Zhang, Y., Bian, K., Wang, C., Xie, X., Wang, H., et al. (2022) Is It Possible to Efficiently and Sustainably Remove Microplastics from Sediments Using Froth Flotation? Chemical Engineering Journal, 448, Article 137692. [Google Scholar] [CrossRef
[15] Geyer, R., Jambeck, J.R. and Law, K.L. (2017) Production, Use, and Fate of All Plastics Ever Made. Science Advances, 3, Article 1700782. [Google Scholar] [CrossRef] [PubMed]
[16] Astorayme, M.A., Vázquez-Rowe, I. and Kahhat, R. (2024) The Use of Artificial Intelligence Algorithms to Detect Macroplastics in Aquatic Environments: A Critical Review. Science of The Total Environment, 945, Article 173843. [Google Scholar] [CrossRef] [PubMed]
[17] Löder, M.G.J. and Gerdts, G. (2015) Methodology Used for the Detection and Identification of Microplastics—A Critical Appraisal. In: Bergmann, M., Gutow, L. and Klages, M., Eds., Marine Anthropogenic Litter, Springer International Publishing, 201-227. [Google Scholar] [CrossRef
[18] Rocha-Santos, T. and Duarte, A.C. (2015) A Critical Overview of the Analytical Approaches to the Occurrence, the Fate and the Behavior of Microplastics in the Environment. TrAC Trends in Analytical Chemistry, 65, 47-53. [Google Scholar] [CrossRef
[19] Karami, A., Golieskardi, A., Keong Choo, C., Larat, V., Galloway, T.S. and Salamatinia, B. (2017) The Presence of Microplastics in Commercial Salts from Different Countries. Scientific Reports, 7, Article No. 46173. [Google Scholar] [CrossRef] [PubMed]
[20] Filella, M. (2015) Questions of Size and Numbers in Environmental Research on Microplastics: Methodological and Conceptual Aspects. Environmental Chemistry, 12, 527-538. [Google Scholar] [CrossRef
[21] Faure, F., Saini, C., Potter, G., Galgani, F., de Alencastro, L.F. and Hagmann, P. (2015) An Evaluation of Surface Micro-and Mesoplastic Pollution in Pelagic Ecosystems of the Western Mediterranean Sea. Environmental Science and Pollution Research, 22, 12190-12197. [Google Scholar] [CrossRef] [PubMed]
[22] Herb, F., Boley, M. and Fong, W. (2025) Machine Learning Outperforms Humans in Microplastic Characterization and Reveals Human Labelling Errors in FTIR Data. Journal of Hazardous Materials, 487, Article 136989. [Google Scholar] [CrossRef] [PubMed]
[23] Dris, R., Gasperi, J., Rocher, V., Saad, M., Renault, N. and Tassin, B. (2015) Microplastic Contamination in an Urban Area: A Case Study in Greater Paris. Environmental Chemistry, 12, 592-599. [Google Scholar] [CrossRef
[24] Desforges, J.W., Galbraith, M., Dangerfield, N. and Ross, P.S. (2014) Widespread Distribution of Microplastics in Subsurface Seawater in the NE Pacific Ocean. Marine Pollution Bulletin, 79, 94-99. [Google Scholar] [CrossRef] [PubMed]
[25] Browne, M.A., Crump, P., Niven, S.J., Teuten, E., Tonkin, A., Galloway, T., et al. (2011) Accumulation of Microplastic on Shorelines Woldwide: Sources and Sinks. Environmental Science & Technology, 45, 9175-9179. [Google Scholar] [CrossRef] [PubMed]
[26] Li, F., Liu, D., Guo, X., Zhang, Z., Martin, F.L., Lu, A., et al. (2024) Identification and Visualization of Environmental Microplastics by Raman Imaging Based on Hyperspectral Unmixing Coupled Machine Learning. Journal of Hazardous Materials, 465, Article 133336. [Google Scholar] [CrossRef] [PubMed]
[27] Haram, L.E., Carlton, J.T., Centurioni, L., Choong, H., Cornwell, B., Crowley, M., et al. (2023) Extent and Reproduction of Coastal Species on Plastic Debris in the North Pacific Subtropical Gyre. Nature Ecology & Evolution, 7, 687-697. [Google Scholar] [CrossRef] [PubMed]
[28] Chong, F., Spencer, M., Maximenko, N., Hafner, J., McWhirter, A.C. and Helm, R.R. (2023) High Concentrations of Floating Neustonic Life in the Plastic-Rich North Pacific Garbage Patch. PLOS Biology, 21, e3001646. [Google Scholar] [CrossRef] [PubMed]
[29] Nuelle, M., Dekiff, J.H., Remy, D. and Fries, E. (2014) A New Analytical Approach for Monitoring Microplastics in Marine Sediments. Environmental Pollution, 184, 161-169. [Google Scholar] [CrossRef] [PubMed]
[30] Technical Subgroup on Marine Litter (MSFD TSG ML) (2011) Marine Litter-Technical recommendations for the Implementation of MSFD Requirements. European Commission.
[31] Scopetani, C., Chelazzi, D., Mikola, J., Leiniö, V., Heikkinen, R., Cincinelli, A., et al. (2020) Olive Oil-Based Method for the Extraction, Quantification and Identification of Microplastics in Soil and Compost Samples. Science of The Total Environment, 733, Article 139338. [Google Scholar] [CrossRef] [PubMed]
[32] Zuo, Z.J., Wang, J., Wang, Y.H. and Xu, M.X. (2023) Research on Soil Microplastic Separation Methods Based on Density Flotation and Oil Extraction. China Environmental Science, 43, Article 5954.
[33] Park, J.W., Lee, J.H. and Kim, D.K. (2020) Froth Flotation of Plastic Particles. Journal of Environmental Engineering, 132, 647-653.
[34] Zhang, Y.C., Liu, Z.Q. and Wang, M.F. (2021) Comparison of Froth Flotation with Density Separation for Microplastic Recovery. Environmental Science and Technology, 54, 7542-7550.
[35] 王昆, 林坤德, 袁东星. 环境样品中微塑料的分析方法研究进展[J]. 环境化学, 2017, 36(1): 27-36.
[36] Cole, M., Webb, H., Lindeque, P.K., Fileman, E.S., Halsband, C. and Galloway, T.S. (2014) Isolation of Microplastics in Biota-Rich Seawater Samples and Marine Organisms. Scientific Reports, 4, Article No. 4528. [Google Scholar] [CrossRef] [PubMed]
[37] 郭静芳. 离子色谱法在常规水环境检测分析中的常见问题及处理方法探讨[J/OL]. 中文科技期刊数据库(全文版)自然科学, 2022(4): 166-169.
http://dianda.cqvip.com/Qikan/Article/Detail?id=1000003412559, 2026-03-13.
[38] Song, Y.K., Hong, S.H., Jang, M., Han, G.M., Rani, M., Lee, J., et al. (2015) A Comparison of Microscopic and Spectroscopic Identification Methods for Analysis of Microplastics in Environmental Samples. Marine Pollution Bulletin, 93, 202-209. [Google Scholar] [CrossRef] [PubMed]
[39] Wang, J. (2021) Scanning Electron Microscopy in the Analysis of Microplastic Morphology. Journal of Applied Microscopy, 54, 98-105.
[40] Liu, B., Zhang, J. and Li, Y. (2020) Microplastic Identification and Analysis Using SEM-EDX. Marine Pollution Bulletin, 95, 234-240.
[41] Zhang, C., Wang, X. and Li, M. (2019) Challenges in the SEM Analysis of Microplastics. Marine Environmental Re-search, 142, 100-106.
[42] Yang, L.Y. (2018) Combining SEM and FTIR for Microplastic Identification. Environmental Science & Technology, 55, 2456-2463.
[43] Hou, H.Y., Dong, M.L., Wang, Y.K., et al. (2016) Rapid and Noninvasive Detection of Skin Cholesterol with Diffuse Reflectance Spectroscopy Technology. Spectroscopy and Spectral Analysis, 36, 3215-3221.
[44] Yang, R., Dong, X., Chen, G., Lin, F., Huang, Z., Manzo, M., et al. (2020) Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose. Polymers, 13, Article 6. [Google Scholar] [CrossRef] [PubMed]
[45] Smith, J.P. (2020) Application of Raman Spectroscopy for Microplastic Detection. Environmental Chemistry, 45, 659-666.
[46] Li, Y.X. and Zhang, H. (2021) Comparison of Raman and FTIR for Microplastic Analysis. Marine Pollution Bulletin, 142, 223-229.
[47] Wang, S. and Liu, J. (2019) Advantages of Raman Spectroscopy in Microplastic Identification. Journal of Analytical Chemistry, 31, 413-419.
[48] Yang, L. (2018) Overcoming Fluorescence Interference in Raman Spectroscopy for Microplastic Analysis. Environmental Science & Technology, 52, 8763-8769.
[49] M. Khedre, A., A. Ramadan, S., Ashry, A. and Alaraby, M. (2023) Pollution of Freshwater Ecosystems by Microplastics: A Short Review on Degradation, Distribution, and Interaction with Aquatic Biota. Sohag Journal of Sciences, 8, 289-295. [Google Scholar] [CrossRef
[50] Liu, Y., Yao, W., Qin, F., Zhou, L. and Zheng, Y. (2023) Spectral Classification of Large-Scale Blended (Micro)Plastics Using FT-IR Raw Spectra and Image-Based Machine Learning. Environmental Science & Technology, 57, 6656-6663. [Google Scholar] [CrossRef] [PubMed]
[51] 杨颖, 吴玉暄, 潘巍, 等. 气相色谱/质谱和红外光谱测定鱼胃肠道内微塑料[J]. 中国环境科学, 2025, 45(7): 4012-4020.
[52] Setälä, O., Fleming-Lehtinen, V. and Lehtiniemi, M. (2014) Ingestion and Transfer of Microplastics in the Planktonic Food Web. Environmental Pollution, 185, 77-83. [Google Scholar] [CrossRef] [PubMed]
[53] Kozlov, M. (2024) Landmark Study Links Microplastics to Serious Health Problems. Nature. [Google Scholar] [CrossRef] [PubMed]
[54] Hu, B., Dai, Y., Zhou, H., Sun, Y., Yu, H., Dai, Y., et al. (2024) Using Artificial Intelligence to Rapidly Identify Microplastics Pollution and Predict Microplastics Environmental Behaviors. Journal of Hazardous Materials, 474, Article 134865. [Google Scholar] [CrossRef] [PubMed]
[55] Gebejes, A., Hrovat, B., Semenov, D., Kanyathare, B., Itkonen, T., Keinänen, M., et al. (2024) Hyperspectral Imaging for Identification of Irregular-Shaped Microplastics in Water. Science of The Total Environment, 944, Article 173811. [Google Scholar] [CrossRef] [PubMed]
[56] Serranti, S., Palmieri, R., Bonifazi, G. and Cózar, A. (2018) Characterization of Microplastic Litter from Oceans by an Innovative Approach Based on Hyperspectral Imaging. Waste Management, 76, 117-125. [Google Scholar] [CrossRef] [PubMed]
[57] Huang, H., Sun, Z., Liu, S., Di, Y., Xu, J., Liu, C., et al. (2021) Underwater Hyperspectral Imaging for in Situ Underwater Microplastic Detection. Science of The Total Environment, 776, Article 145960. [Google Scholar] [CrossRef
[58] Xue, R., Lan, R., Su, W., Wang, Z., Li, X., Zhao, J., et al. (2023) Mechanistic Understanding toward the Maternal Transfer of Nanoplastics in daphnia Magna. ACS Nano, 17, 13488-13499. [Google Scholar] [CrossRef] [PubMed]
[59] Serranti, S., Capobianco, G., Cucuzza, P. and Bonifazi, G. (2024) Efficient Microplastic Identification by Hyperspectral Imaging: A Comparative Study of Spatial Resolutions, Spectral Ranges and Classification Models to Define an Optimal Analytical Protocol. Science of The Total Environment, 954, Article 176630. [Google Scholar] [CrossRef] [PubMed]
[60] Van Cauwenberghe, L., Vanreusel, A., Mees, J. and Janssen, C.R. (2013) Microplastic Pollution in Deep-Sea Sediments. Environmental Pollution, 182, 495-499. [Google Scholar] [CrossRef] [PubMed]
[61] Eriksen, M., Mason, S., Wilson, S., Box, C., Zellers, A., Edwards, W., et al. (2013) Microplastic Pollution in the Surface Waters of the Laurentian Great Lakes. Marine Pollution Bulletin, 77, 177-182. [Google Scholar] [CrossRef] [PubMed]
[62] Dekiff, J.H., Remy, D., Klasmeier, J. and Fries, E. (2014) Occurrence and Spatial Distribution of Microplastics in Sediments from Norderney. Environmental Pollution, 186, 248-256. [Google Scholar] [CrossRef] [PubMed]
[63] Dümichen, E., Barthel, A., Braun, U., Bannick, C.G., Brand, K., Jekel, M., et al. (2015) Analysis of Polyethylene Microplastics in Environmental Samples, Using a Thermal Decomposition Method. Water Research, 85, 451-457. [Google Scholar] [CrossRef] [PubMed]
[64] Russell, M. and Webster, L. (2021) Microplastics in Sea Surface Waters around Scotland. Marine Pollution Bulletin, 166, Article 112210. [Google Scholar] [CrossRef] [PubMed]
[65] Cheung, P.K., Fok, L., Hung, P.L. and Cheung, L.T.O. (2018) Science of The Total Environment, 628, 731-739. [Google Scholar] [CrossRef
[66] Setälä, O., Magnusson, K., Lehtiniemi, M. and Norén, F. (2016) Distribution and Abundance of Surface Water Microlitter in the Baltic Sea: A Comparison of Two Sampling Methods. Marine Pollution Bulletin, 110, 177-183. [Google Scholar] [CrossRef] [PubMed]
[67] 曲玲, 张微微, 王旭, 郭洪发, 陈元, 明浩, 张守锋, 赵骞, 王菊英. 锦州湾表层海水微塑料分布特征[J]. 海洋学报, 2021, 43(2): 98-104.
[68] Aliabad, M.K., Nassiri, M. and Kor, K. (2019) Microplastics in the Surface Seawaters of Chabahar Bay, Gulf of Oman (Makran Coasts). Marine Pollution Bulletin, 143, 125-133. [Google Scholar] [CrossRef] [PubMed]
[69] Olivatto, G.P., Martins, M.C.T., Montagner, C.C., Henry, T.B. and Carreira, R.S. (2019) Microplastic Contamination in Surface Waters in Guanabara Bay, Rio De Janeiro, Brazil. Marine Pollution Bulletin, 139, 157-162. [Google Scholar] [CrossRef] [PubMed]
[70] 李征, 高春梅, 杨金龙, 等. 连云港海州湾海域表层水体和沉积物中微塑料的分布特征[J]. 环境科学, 2020, 41(7): 3212-3221.
[71] Fondation Tara Océan (2022) Chemical Composition of Microplastics Floating on the Surface of the Mediterranean Sea. Marine Pollution Bulletin, 174, Article 113284.
https://preprod.taraocean.aquaray.com//app/uploads/2022/01/chemical-composition-of-microplastics-floating-on-the-surface-of-the-mediterranean-sea.pdf
[72] Ding, R., Li, Q., Wang, K., Tian, J., Lu, L., Li, W., et al. (2024) Occurrence and Distribution of Microplastics in the Adjacent Environment of Yellow River Delta, China. Marine Pollution Bulletin, 199, Article 116019. [Google Scholar] [CrossRef] [PubMed]
[73] Fries, E., Dekiff, J.H., Willmeyer, J., Nuelle, M., Ebert, M. and Remy, D. (2013) Identification of Polymer Types and Additives in Marine Microplastic Particles Using Pyrolysis-GC/MS and Scanning Electron Microscopy. Environmental Science: Processes & Impacts, 15, 1949-1956. [Google Scholar] [CrossRef] [PubMed]
[74] Crichton, E.M., Noël, M., Gies, E.A. and Ross, P.S. (2017) A Novel, Density-Independent and FTIR-Compatible Approach for the Rapid Extraction of Microplastics from Aquatic Sediments. Analytical Methods, 9, 1419-1428. [Google Scholar] [CrossRef
[75] Fok, L. and Cheung, P.K. (2015) Marine Pollution Bulletin, 99, 112-118. [Google Scholar] [CrossRef
[76] Benassi, G., et al. (2017) Characterization of Microplastic Particles in the Environment Using AFM-IR. Environmental Science & Technology, 51, 7433-7440.
[77] Zhou, X., et al. (2020) AFM-IR for the Detection and Characterization of Nano-Sized Plastics in the Environment. Nature Communications, 11, 1-9.
[78] Jiang, X., et al. (2019) Application of Field-Flow Fractionation (FFF) in the Analysis of Nanoplastics in Marine Environments. Environmental Science & Technology, 53, 2005-2012.