载茜素明胶–透明质酸水凝胶的构建及止血性能初步评价

Preparation of Alizarin-Loaded Gelatin-Hyaluronic Acid Hydrogel and Preliminary Evaluation of Its Hemostatic Performance

摘要: 目的：制备负载中药茜素的明胶/透明质酸水凝胶复合止血材料(Al@Ge/HA)，并对其性能进行评价。方法：采用双注射器系统进行原位交联制备Al@Ge/HA，溶胀测试其吸水膨胀率，进行水凝胶黏附测试，体外凝血初步检测止血能力，并通过SD大鼠尾出血和肝脏出血模型检测其快速止血效果。结果：制备了Al@Ge/HA，具有较好的吸水膨胀功能，可浓缩血液实现快速止血，且具有良好的血液相容性。止血实验表明，Al@Ge/HA对大鼠尾损伤和肝脏损伤的止血时间和止血效果均优于空白对照组。结论：Al@Ge/HA具有良好的止血效果和血液相容性，具有进一步研究的价值和临床应用前景。

Abstract: Objective: To prepare a gelatin/hyaluronic acid hydrogel composite hemostatic material loaded with alizarin (Al@Ge/HA) and evaluate its properties. Methods: Al@Ge/HA was prepared using a dual-syringe system for in situ crosslinking. Its water absorption and swelling rate were tested through swelling experiments, and its adhesive properties were assessed via hydrogel adhesion tests. Preliminary in vitro blood clotting assays were conducted to evaluate its hemostatic capability. The rapid hemostatic effect was further examined using SD rat tail bleeding and liver bleeding models. Results: Al@Ge/HA was successfully prepared, demonstrating favorable water absorption and swelling functions, which concentrated blood and achieved rapid hemostasis. It also exhibited good blood compatibility. Hemostatic experiments indicated that Al@Ge/HA outperformed the blank control group in terms of hemostatic time and efficacy for both rat tail and liver injuries. Conclusion: Al@Ge/HA exhibits excellent hemostatic performance and blood compatibility, holding significant value for further research and clinical application prospects.