基于UPLC-MS/MS技术及生物信息学探究藤三七抗胃溃疡的物质基础及作用机制
Exploring the Material Basis and Mechanism of Anredera cordifolia (Ten.) Sreenis against Gastric Ulcer Based on UPLC-MS/MS and Bioinformatics
DOI: 10.12677/pi.2026.152017, PDF,    科研立项经费支持
作者: 方镕泽, 黄燕秋, 欧阳若水, 陈 岭, 敬美莲, 黄敏洁*：广东江门中医药职业学院，广东 江门
关键词: 胃溃疡；藤三七；UPLC-MS/MS；网络药理学；Gastric Ulcer； Anredera cordifolia (Ten.) Sreenis； UPLC-MS/MS； Network Pharmacology
摘要: 目的：明确藤三七抗胃溃疡(GU)的药效物质及其作用机制。方法：UPLC-Q-TOF-MS鉴定藤三七水提液成分，在TCMSP、GeneCards筛选活性成分与疾病靶点，Venny取交集后构建PPI网络，Cytoscape拓扑分析获取核心靶点。DAVID进行GO与KEGG富集。结果：共鉴定出藤三七成分160种，其中40个符合OB > 30%、DL > 0.18，映射239个GU相关靶点。TNF、IL-6、AKT1、TP53、IL-1β为度值前五位核心靶点；IL-17与TNF信号通路富集最显著。结论：藤三七通过多成分协同调控IL-17/TNF通路，抑制炎症与氧化应激发挥抗胃溃疡作用。
Abstract: Objective: To identify the bioactive constituents and potential mechanisms of Anredera cordifolia (Ten.) Sreenis in the treatment of gastric ulcer (GU). Methods: Components of the aqueous extract of Anredera cordifolia (Ten.) Sreenis were characterized by UPLC-Q-TOF-MS. Putative active ingredients and GU-related targets were retrieved from TCMSP and GeneCards, respectively. Intersection targets were imported into a Protein-Protein Interaction (PPI) network constructed in Cytoscape. Topological analysis was applied to screen core targets. GO and KEGG enrichment analyses were performed with DAVID. Results: A total of 160 constituents were identified, 40 of which met the criteria of Oral Bioavailability (OB) > 30% and Drug-Likeness (DL) > 0.18. These compounds mapped to 239 GU-associated targets. The top five hub genes ranked by degree were TNF, IL-6, AKT1, TP53 and IL-1β. The IL-17 and TNF signaling pathways were the most significantly enriched. Conclusion: Anredera cordifolia (Ten.) Sreenis exerts anti-gastric-ulcer effects through multi-component synergy that modulates the IL-17/TNF pathway, thereby suppressing inflammation and oxidative stress.
文章引用：方镕泽, 黄燕秋, 欧阳若水, 陈岭, 敬美莲, 黄敏洁. 基于UPLC-MS/MS技术及生物信息学探究藤三七抗胃溃疡的物质基础及作用机制 [J]. 药物资讯, 2026, 15(2): 144-153. https://doi.org/10.12677/pi.2026.152017

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