放养密度对鲢鱼淀粉酶和脂肪酶活性的影响

Influence of Breeding Density on Amylase Activity and Lipase Activity of Hypophthalmichthys molitrix

摘要: 本实验就高密度养殖与低密度养殖下对鱼体内淀粉酶和脂肪酶活性的影响进行了探究。2013年4月~10月，在山西省永济市温流水良种繁育场设置了两组池塘，每组三个，一组为低密度鲢鱼养殖(21.2 g•m−3)，一组为高密度鲢鱼养殖(42.4 g•m−3)。对此两种密度养殖下鲢鱼肠道淀粉酶和脂肪酶活性进行了测定，结果表明：高密度养殖池塘中鲢鱼肠道的淀粉酶活力和脂肪酶活力均高于低密度养殖池塘，且鲢鱼前肠此两种消化酶活性高于后肠；高密度鲢鱼养殖池塘与低密度相比，蓝藻门的细胞丰度明显下降，绿藻门细胞丰度所占百分比明显增加；淀粉酶活力与蓝藻的细胞丰度呈负相关，与其余门类浮游植物细胞丰度呈正相关，脂肪酶活力与蓝藻和裸藻的细胞丰度呈负相关，与其余门类浮游植物细胞丰度呈正相关。

Abstract: This experiment has carried on exploration including the influence on amylase and lipase activities in fish body under high and low breeding density. From April to October in 2013, the influence of different breeding densities on amylase activity and lipase activity in H. molitrix intestinal tract was investigated in Wenliushui excellent fish breeding grounds in Yongji, Shanxi province, North China. Two experimental groups (each including three ponds) were set, which represent low and high breeding of H. molitrix density of 21.2 g•m−3 and 42.4 g•m−3 respectively. The results showed that amylase activity of high density breeding ponds was higher than that of low density breeding ponds, and amylase activity in foregut is higher than that in hindgut, as is the same with the lipase activity. The cell abundance of Cyanophyta in high breeding density ponds was obviously lower, while the proportion of the cell abundance of Chlorophyta was higher than that in low breeding density ponds. The amylase activity had a negative correlation with the cell abundance of Cyanophyta but a positive correlation with the cell abundance of the other divisions. The lipase activity had a negative correlation with the cell abundance of Cyanophyta and Euglenophyta, but a positive correlation with the cell abundance of the other divisions.