羟基酪醇在制备、抗皮肤衰老方面的研究 与应用进展
Research and Application Progress of Hydroxytyrosol in Preparation and Anti-Aging of the Skin
摘要: 近年来，羟基酪醇(hydroxytyrosol, HT)作为橄榄来源的天然酚类化合物，凭借优异的抗衰老活性，在化妆品活性成分研究领域备受关注，成为开发高效、绿色抗衰产品的核心研究对象。基于国内外相关文献，本文旨在具体综述HT的制备技术与皮肤抗衰医学研究的进展，包括分析植物提取、化学合成及生物合成三大路径的技术优势与局限，深入阐述HT通过调控氧化应激、抑制炎症反应及诱导细胞自噬等多维度发挥抗皮肤衰老的作用机制与市场应用现状。最后，总结并指出现阶段研究仍然存在的问题，并对该成分在皮肤健康领域的未来发展方向进行了展望，旨在为该成分在皮肤医学研发领域的深度研究与产业化应用提供参考。
Abstract: In recent years, hydroxytyrosol (HT), a natural phenolic compound derived from olives, has attracted significant attention in the field of active cosmetic ingredients due to its excellent anti-aging properties, becoming a key research focus for developing efficient and eco-friendly anti-aging products. Based on relevant domestic and international literature, this article aims to specifically review the preparation techniques and advancements in skin anti-aging medical research of HT, including a detailed analysis of the technical advantages and limitations of the three main approaches: plant extraction, chemical synthesis, and biosynthesis. It further elaborates on the multidimensional mechanisms by which HT exerts anti-skin aging effects, such as regulating oxidative stress, inhibiting inflammatory responses, and inducing cellular autophagy, as well as its current market applications. Finally, the article summarizes existing research challenges and provides insights into future development directions for this compound in the field of skin health, aiming to offer reference for in-depth research and industrial application of HT in dermatological medicine.
文章引用：唐嘉, 张兴江, 孙丹丹, 刘睿颖, 黄庆, 吴建新. 羟基酪醇在制备、抗皮肤衰老方面的研究 与应用进展[J]. 临床医学进展, 2026, 16(4): 561-569. https://doi.org/10.12677/acm.2026.1641281

参考文献

[1] Bertelli, M., Kiani, A.K., Paolacci, S., Manara, E., Kurti, D., Dhuli, K., et al. (2020) Hydroxytyrosol: A Natural Compound with Promising Pharmacological Activities. Journal of Biotechnology, 309, 29-33. [Google Scholar] [CrossRef] [PubMed]
[2] 李春燕. 油橄榄叶中羟基酪醇的提取及橄榄酒的研制[D]: [硕士学位论文]. 成都: 西华大学, 2014.
[3] 罗成, 陈明智, 李艳. 羟基酪醇的药理作用研究进展[J]. 中国药学杂志, 2019, 54(20): 1642-1646.
[4] 张燕琴, 曹健. 新型天然活性物质羟基酪醇的研究进展[J]. 成都医学院学报, 2016, 11(2): 270-272.
[5] 臧皓, 张露云, 徐倩, 等. 羟基酪醇药理作用研究的新进展[J]. 人参研究, 2021, 33(1): 54-59.
[6] Wong, Y.L., Boulos, S. and Nyström, L. (2025) The Olive Biophenol Hydroxytyrosol in Neutral Aqueous Solutions—A UPLC-MS/MS Investigation of Its Stability and Oxidative Color Formation. Frontiers in Nutrition, 12, Article 1532087. [Google Scholar] [CrossRef] [PubMed]
[7] 原姣姣, 叶建中, 王成章, 等. 羟基酪醇的热稳定性和分解动力学研究[J]. 林产化学与工业, 2016, 36(6): 87-92.
[8] 杨子琪, 龙建纲, 刘健康. 羟基酪醇的生物学活性及其代谢特征[J]. 中国药理学通报, 2016, 32(9): 1189-1193.
[9] Karković Marković, A., Torić, J., Barbarić, M. and Jakobušić Brala, C. (2019) Hydroxytyrosol, Tyrosol and Derivatives and Their Potential Effects on Human Health. Molecules, 24, Article 2001. [Google Scholar] [CrossRef] [PubMed]
[10] 徐保鑫, 李转梅, 张学兰, 等. 女贞子中橄榄苦苷与羟基酪醇在大鼠血浆中的药动学差异[J]. 中成药, 2017, 39(7): 1387-1390.
[11] 鞠星可. 中药青果中羟基酪醇的提取、衍生化及活性研究[D]: [硕士学位论文]. 西安: 陕西科技大学, 2021.
[12] 张邦全, 胡静. 油橄榄提取物的应用探讨[J]. 口腔护理用品工业, 2018, 28(2): 16-20.
[13] 刘香萍. 紫丁香主要活性成分制备及抗氧化应激活性评价[D]: [博士学位论文]. 哈尔滨: 东北林业大学, 2013.
[14] 田立鹏. 油橄榄叶提取物的制备及生物活性研究[D]: [硕士学位论文]. 兰州: 甘肃农业大学, 2022.
[15] 郭晓强, 曹敏, 梁立, 等. 油橄榄叶水提物的有效成分与抗氧化活性研究[J]. 食品研究与开发, 2019, 40(10): 26-31.
[16] Borjan, D., Leitgeb, M., Knez, Ž. and Hrnčič, M.K. (2020) Microbiological and Antioxidant Activity of Phenolic Compounds in Olive Leaf Extract. Molecules, 25, Article 5946. [Google Scholar] [CrossRef] [PubMed]
[17] 王玉洁. 油橄榄叶中羟基酪醇的制备及其抗氧化活性研究[D]: [硕士学位论文]. 雅安: 四川农业大学, 2020.
[18] Britton, J., Davis, R. and O’Connor, K.E. (2019) Chemical, Physical and Biotechnological Approaches to the Production of the Potent Antioxidant Hydroxytyrosol. Applied Microbiology and Biotechnology, 103, 5957-5974. [Google Scholar] [CrossRef] [PubMed]
[19] Gao, Y., Fan, M., Cheng, X., Liu, X., Yang, H., Ma, W., et al. (2024) Deep Eutectic Solvent: Synthesis, Classification, Properties and Application in Macromolecular Substances. International Journal of Biological Macromolecules, 278, Article ID: 134593. [Google Scholar] [CrossRef] [PubMed]
[20] 刘月含, 吕明俊, 姜晓坤. 超声辅助低共熔溶剂提取油橄榄果渣中羟基酪醇的工艺优化研究[J]. 东北农业科学, 2024, 49(2): 102-107.
[21] Wang, E., Jiang, Y. and Zhao, C. (2024) Hydroxytyrosol Isolation, Comparison of Synthetic Routes and Potential Biological Activities. Food Science & Nutrition, 12, 6899-6912. [Google Scholar] [CrossRef] [PubMed]
[22] Kalampaliki, A.D., Giannouli, V., Skaltsounis, A. and Kostakis, I.K. (2019) A Three-Step, Gram-Scale Synthesis of Hydroxytyrosol, Hydroxytyrosol Acetate, and 3,4-Dihydroxyphenylglycol. Molecules, 24, Article 3239. [Google Scholar] [CrossRef] [PubMed]
[23] Ziosi, P., Paolucci, C., Santarelli, F., Tabanelli, T., Passeri, S., Cavani, F., et al. (2018) A Two‐Step Process for the Synthesis of Hydroxytyrosol. ChemSusChem, 11, 2202-2210. [Google Scholar] [CrossRef] [PubMed]
[24] 刘英梅, 郭新艳, 张秀华, 等. 响应面法优化羟化酶催化酪醇合成羟基酪醇工艺[J]. 化学与生物工程, 2024, 41(7): 44-49, 55.
[25] 汪鑫, 孙涛, 孙美莉, 等. 生物合成功能性食品原料羟基酪醇的研究进展[J]. 生物技术通报, 2025, 41(11): 35-46.
[26] Liu, Y., Liu, H., Hu, H., Ng, K.R., Yang, R. and Lyu, X. (2022) de Novo Production of Hydroxytyrosol by Metabolic Engineering of saccharomyces Cerevisiae. Journal of Agricultural and Food Chemistry, 70, 7490-7499. [Google Scholar] [CrossRef] [PubMed]
[27] Deri-Zenaty, B., Bachar, S., Rebroš, M. and Fishman, A. (2020) A Coupled Enzymatic Reaction of Tyrosinase and Glucose Dehydrogenase for the Production of Hydroxytyrosol. Applied Microbiology and Biotechnology, 104, 4945-4955. [Google Scholar] [CrossRef] [PubMed]
[28] Zhang, C., Gao, X., Li, M., Yu, X., Huang, F., Wang, Y., et al. (2023) The Role of Mitochondrial Quality Surveillance in Skin Aging: Focus on Mitochondrial Dynamics, Biogenesis and Mitophagy. Ageing Research Reviews, 87, Article ID: 101917. [Google Scholar] [CrossRef] [PubMed]
[29] Böhm, M., Stegemann, A., Paus, R., Kleszczyński, K., Maity, P., Wlaschek, M., et al. (2025) Endocrine Controls of Skin Aging. Endocrine Reviews, 46, 349-375. [Google Scholar] [CrossRef] [PubMed]
[30] 吕永博, 曲召辉, 宋阳, 等. 皮肤氧化损伤途径及皮肤抗氧化屏障系统[J]. 香料香精化妆品, 2025(5): 23-30.
[31] Bovicelli, P. (2007) Radical-Scavenging Polyphenols: New Strategies for Their Synthesis. Journal of Pharmacy and Pharmacology, 59, 1703-1710. [Google Scholar] [CrossRef] [PubMed]
[32] Fitsiou, E., Pulido, T., Campisi, J., Alimirah, F. and Demaria, M. (2021) Cellular Senescence and the Senescence-Associated Secretory Phenotype as Drivers of Skin Photoaging. Journal of Investigative Dermatology, 141, 1119-1126. [Google Scholar] [CrossRef] [PubMed]
[33] Avola, R., Graziano, A.C.E., Pannuzzo, G., Bonina, F. and Cardile, V. (2018) Hydroxytyrosol from Olive Fruits Prevents Blue‐Light‐Induced Damage in Human Keratinocytes and Fibroblasts. Journal of Cellular Physiology, 234, 9065-9076. [Google Scholar] [CrossRef] [PubMed]
[34] 戴韵佳, 张建中, 邓丹琪, 曹文婷. 炎症与皮肤衰老: 机制、干预与未来展望[J]. 中国皮肤性病学杂志, 2026, 40(3): 237-243.
[35] Smeriglio, A., Denaro, M., Mastracci, L., Grillo, F., Cornara, L., Shirooie, S., et al. (2019) Safety and Efficacy of Hydroxytyrosol-Based Formulation on Skin Inflammation: In Vitro Evaluation on Reconstructed Human Epidermis Model. DARU Journal of Pharmaceutical Sciences, 27, 283-293. [Google Scholar] [CrossRef] [PubMed]
[36] Liu, M., Fan, R., Chen, L., Zhang, D. and Chen, C. (2025) Hydroxytyrosol Ameliorates Imiquimod-Induced Psoriasis-Like Dermatitis by Modulating ERK and NF-κB Signaling Pathways in Mice. Scientific Reports, 15, Article No. 30125. [Google Scholar] [CrossRef] [PubMed]
[37] Wang, J., Yuan, M., Li, Q., Shen, C., Zhang, X., Zhu, C., et al. (2025) Combined Protection against UVB-Induced Photoaging by Oleuropein, Hydroxytyrosol, and Verbascoside through Modulation of Inflammation, Oxidative Stress, and Collagen Homeostasis. Scientific Reports, 15, Article No. 41008. [Google Scholar] [CrossRef] 
[38] Eckhart, L., Tschachler, E. and Gruber, F. (2019) Autophagic Control of Skin Aging. Frontiers in Cell and Developmental Biology, 7, Article 143. [Google Scholar] [CrossRef] [PubMed]
[39] Chen, Q., Sun, T., Wang, J., Jia, J., Yi, Y., Chen, Y., et al. (2019) Hydroxytyrosol Prevents Dermal Papilla Cells Inflammation under Oxidative Stress by Inducing Autophagy. Journal of Biochemical and Molecular Toxicology, 33, e22377. [Google Scholar] [CrossRef] [PubMed]
[40] 广州腾麟生物科技有限公司. 一种敏感肌适用的抗衰老面霜及其制备方法[P]. 中国专利, CN121059456A. 2025-12-05.
[41] 北京青颜博识健康管理有限公司, 北京大学. 一种可溶性纳米纤维微针透皮贴片、其制备方法及应用[P]. 中国专利, CN121287590A. 2026-01-09.
[42] 江宇航. 共晶技术改善羟基酪醇的物理性状及稳定性[D]: [硕士学位论文]. 上海: 中国科学院上海药物研究所, 2021.
[43] Martins, B.T., Faria, N.A., Macedo, A.C., Miragaia, M., Serra, A.T., Bronze, M.R., et al. (2024) Exploring the Biological Potential of Hydroxytyrosol and Derivatives: Synthetic Strategies and Evaluation of Antiproliferative, Antioxidant, and Antimicrobial Activities. Journal of Agricultural and Food Chemistry, 72, 26699-26710. [Google Scholar] [CrossRef] [PubMed]
[44] Zhang, X., Wang, P., Hu, Y., Ke, H., Wang, R., Zhang, X., et al. (2024) The Impact of Cistanche on the Therapeutic Potential of Acne after Fermentation with Beauveria bassiana. Food Science and Human Wellness. [Google Scholar] [CrossRef] 

为你推荐