白介素37对紫杉醇耐药肺腺癌细胞生物学行为的影响
The Effect of Interleukin-37 on the Biological Behaviour of Paclitaxel-Resistant Lung Adenocarcinoma Cells
DOI: 10.12677/acm.2026.1641340, PDF,   
作者: 唐 骏：青岛大学青岛医学院，山东 青岛；毕庆庆*：康复大学青岛中心医院临床检验科，山东 青岛
关键词: 白介素37；紫杉醇；肺腺癌；非小细胞肺癌；Interleukin-37； Paclitaxel； Lung Adenocarcinoma； Non-Small Cell Lung Cancer
摘要: 目的：本研究旨在探究白介素37 (IL-37)对紫杉醇耐药肺腺癌细胞细胞生物学的影响。方法：利用慢病毒感染构建IL-37过表达的紫杉醇耐药A549 (A549/TAX)细胞系，通过CCK8实验检测A549/TAX细胞对紫杉醇的耐药性以及IL-37对紫杉醇杀伤A549/TAX细胞能力的影响，分别采用克隆形成实验、伤口愈合实验和Transwell侵袭实验研究IL-37对A549/TAX细胞增殖、迁移和侵袭能力的影响，通过荧光染色实验检测细胞内活性氧(ROS)水平，采用实时荧光定量PCR检测IL-37和GAPDH的mRNA水平，使用Western blot检测细胞中IL-37、聚腺苷酸二磷酸核糖聚合酶1 (PARP1)、半胱氨酸天冬氨酸蛋白酶3 (Caspase3)、GAPDH的蛋白表达情况。结果：紫杉醇对A549和A549/TAX细胞均表现出浓度依赖性抗增殖作用。IL-37过表达可以在体外显著增强紫杉醇对A549/TAX细胞的杀伤作用，并抑制A549/TAX细胞的增殖、迁移和侵袭能力。此外，IL-37过表达可以诱导耐药细胞ROS积累并促进细胞凋亡。结论：IL-37过表达抑制A549/TAX细胞增殖、迁移与侵袭能力，还可以诱导细胞活性氧积累和细胞凋亡。
Abstract: Objective: This study aims to investigate the effect of interleukin-37 (IL-37) on the biological behaviors of paclitaxel-resistant lung adenocarcinoma cells. Methods: A paclitaxel-resistant A549 (A549/TAX) cell line overexpressing IL-37 was established using lentiviral infection. The CCK8 assay was performed to evaluate the resistance of A549/TAX cells to paclitaxel and the effect of IL-37 on the cytotoxicity of paclitaxel against A549/TAX cells. Colony formation, wound healing, and Transwell invasion assays were conducted to assess the effects of IL-37 on the proliferation, migration, and invasion capabilities of A549/TAX cells, respectively. Fluorescent staining was used to detect intracellular reactive oxygen species (ROS) levels. Quantitative real-time PCR was employed to measure the mRNA levels of IL-37 and GAPDH. Western blot analysis was performed to examine the protein expression levels of IL-37, poly (ADP-ribose) polymerase 1 (PARP1), caspase-3, and GAPDH in the cells. Results: Paclitaxel exhibited a concentration-dependent anti-proliferative effect on both A549 and A549/TAX cells. Overexpression of IL-37 significantly enhanced the cytotoxic effect of paclitaxel on A549/TAX cells in vitro and inhibited the proliferation, migration, and invasion of A549/TAX cells. Furthermore, IL-37 overexpression induced ROS accumulation and promoted apoptosis in drug-resistant cells. Conclusions: Overexpression of IL-37 inhibits the proliferation, migration, and invasion of A549/TAX cells and induces ROS accumulation and apoptosis in these cells.
文章引用：唐骏, 毕庆庆. 白介素37对紫杉醇耐药肺腺癌细胞生物学行为的影响[J]. 临床医学进展, 2026, 16(4): 1075-1083. https://doi.org/10.12677/acm.2026.1641340

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