减味连梅汤对DSS + LPS诱导的脓毒症小鼠的保护作用

Protective Effect of Jianwei Lianmei Decoction on DSS and LPS-Induced Septic Mice

摘要: 目的：探究连梅汤中的乌梅与黄连配伍(W + H)的减味连梅汤对脓毒症模型小鼠的保护效应。方法：C57BL/6J雄性小鼠经随机化处理后分为3组(n = 6)：CON组、DSS + LPS模型组与W + H治疗组。模型组与W + H治疗组通过腹腔注射LPS (5 mg/kg)联用DSS建立肠损伤相关脓毒症模型。治疗组预先进行21天的减味连梅汤灌胃干预。随后，实验终点评估小鼠一般状态及肠、肝器官损伤状态。利用HE染色评估肠、肝形态学病变，并借助RT-qPCR分析结肠紧密连接蛋白(ZO-1)及肠、肝组织中关键炎性介质(IL-1β, IL-8, IL-17, TNF-α)的表达差异。结果：相比对照组，模型组表现出明显的体重减失及粪便性状异常(P < 0.001)，以及肠、肝器官损伤。减味连梅汤干预显著逆转了上述表型，不仅上调了结肠ZO-1的表达(P < 0.05)，还下调了肠道与肝脏内的促炎因子水平，并有效减轻了组织病理损害。结论：减味连梅汤对脓毒症引起的结肠屏障功能障碍具有修复作用，并能缓解结肠与肝脏的协同损伤。

Abstract: Objective: To investigate the protective effects of the flavor-reduced Lianmei Decoction combined with Wumei and Coptis (W + H) on septic model mice. Methods: Male C57BL/6J mice were randomly allocated into three experimental cohorts: the control (CON) group, the DSS + LPS-induced model group, and the W + H intervention group. The model group and W + H treatment group were subjected to intraperitoneal injection of LPS (5 mg/kg) combined with DSS to induce intestinal injury-related sepsis. The treatment group received a 21-day pre-treatment of flavor-reduced Lianmei Decoction via gavage. Following the intervention, general conditions of the mice and damage to intestinal and hepatic organs were assessed. HE staining was used to evaluate morphological lesions in the intestine and liver, and RT-qPCR was applied to analyze differences in the expression of colonic tight junction protein (ZO-1) and key inflammatory mediators (IL-1β, IL-8, IL-17, TNF-α) in intestinal and hepatic tissues. Results: Compared to the control group, the model group exhibited significant weight loss and abnormal fecal characteristics (P < 0.0001), as well as intestinal and hepatic organ damage. Intervention with flavor-reduced Lianmei Decoction significantly reversed these phenotypes, upregulated colonic ZO-1 expression (P < 0.05), downregulated pro-inflammatory factors in the intestine and liver, and effectively alleviated histopathological damage. Conclusion: Flavor-reduced Lianmei Decoction has a reparative effect on colonic barrier dysfunction induced by sepsis and can mitigate synergistic damage to the colon and liver.