关于消除试纸条钩状效应问题的研究进展
Research Progress on Eliminating the Hook Effect in Test Strips
DOI: 10.12677/acm.2026.1641424, PDF,    科研立项经费支持
作者: 秦一之, 吴茳铃, 崔 曼, 蒋美燕, 刘定华：重庆医科大学附属大学城医院检验科，重庆
关键词: 钩状效应；侧流免疫层析技术；试纸条；免疫测定；Hook Effect； Lateral Flow Immunoassay； Test Strips； Immunoassay
摘要: 侧流免疫层析技术(Lateral Flow Immunoassay, LFIA)是一种快速、简便、成本效益高的检测技术，广泛应用于医学检测、食品安全、环境监测等多个领域。其基于抗原抗体免疫反应，通过试纸条的形式实现对特定生物分子的快速检测。尽管LFIA具有许多优点，但也存在一些技术挑战，譬如钩状效应会影响试纸条诊断灵敏度与检测范围。钩状效应作为临床检验中经常出现的现象越来越被广泛研究，其是指由于抗原抗体比例不合适而导致假阴性的现象。本文基于目前领域中消除试纸条钩状效应的方法及最新进展，总结并为读者提供了在设计试纸条方面新的思路与参考，有助于试纸条的进一步研发与革新。
Abstract: Lateral Flow Immunoassay (LFIA) is a rapid, simple and cost-effective detection technology, which is widely used in many fields such as medical testing, food safety and environmental monitoring. It is based on an antigen-antibody immune reaction and realizes rapid detection of specific biomolecules in the form of test strips. Although LFIA has many advantages, there are some technical challenges, such as the hook effect, which affects the diagnostic sensitivity and detection range of the test strips. The hook effect is increasingly widely studied as a frequent phenomenon in clinical testing, which refers to the phenomenon of false negatives due to inappropriate antigen-antibody ratios. This paper summarizes and provides readers with new ideas and references in designing test strips based on the current methods and recent advances in the field to eliminate the hooking effect, which can help further development and innovation of test strips.
文章引用：秦一之, 吴茳铃, 崔曼, 蒋美燕, 刘定华. 关于消除试纸条钩状效应问题的研究进展[J]. 临床医学进展, 2026, 16(4): 1837-1843. https://doi.org/10.12677/acm.2026.1641424

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