双膦酸盐介导Mg2+缓释的双网络功能性 水凝胶体外骨缺损修复的研究
Bisphosphonate-Mediated Mg2+ Sustained Release Dual-Network Functional Hydrogels for in Vitro Bone Defect Repair
DOI: 10.12677/acm.2026.1641540, PDF,   
作者: 赵人杰, 高倩倩, 王元银*：安徽医科大学口腔医学院，安徽医科大学附属口腔医院，安徽省口腔疾病研究重点实验室，安徽 合肥
关键词: 骨缺损；双膦酸盐；镁离子；水凝胶；血管生成；骨生成；Bone Defect； Bisphosphonate； Magnesium Ion； Hydrogel； Angiogenesis； Osteogenesis
摘要: 目的：探究负载双膦酸盐(BP)与镁离子(Mg2+)的复合水凝胶在促进骨形成、诱导血管新生方面的综合效能，为骨缺损修复提供新型材料策略。方法：以海藻酸钠(SA)与明胶(Gel)为基质，通过接枝BP并引入Mg2+，经化学交联构建多功能复合水凝胶。采用扫描电子显微镜与傅里叶变换红外光谱对支架进行系统表征，并通过体外实验从生物相容性、促血管生成、成骨诱导三个维度全面评估材料性能。结果：复合水凝胶的微观形貌与化学结构均优于单一水凝胶，具有优良的生物相容性，可有效促进新生血管形成与骨组织再生。结论：该复合水凝胶兼具多重生物活性功能，能够在骨重塑调控的多个关键环节协同发挥作用，有望为骨缺损的精准修复提供新的治疗思路与技术参考。
Abstract: Objective: To investigate the combined efficacy of bisphosphonate (BP)-loaded and magnesium ion (Mg2+)-loaded composite hydrogels in promoting bone formation and inducing angiogenesis, providing a novel material strategy for bone defect repair. Methods: A multifunctional composite hydrogel was constructed by chemically crosslinking sodium alginate (SA) and gelatin (Gel) as the matrix, grafting BP, and introducing Mg2+. The scaffolds were systematically characterized using scanning electron microscopy and Fourier transform infrared spectroscopy. Material performance was comprehensively evaluated through in vitro experiments across three dimensions: biocompatibility, proangiogenicity, and osteogenic induction. Results: The composite hydrogel exhibited superior microstructure and chemical composition compared to monocomponent hydrogels, demonstrating excellent biocompatibility and effectively promoting neovascularization and bone tissue regeneration. Conclusion: This composite hydrogel possesses multiple bioactive functions and can synergistically act on several key stages of bone remodeling regulation. It holds promise as a novel therapeutic approach and technical reference for the precise repair of bone defects.
文章引用：赵人杰, 高倩倩, 王元银. 双膦酸盐介导Mg2+缓释的双网络功能性 水凝胶体外骨缺损修复的研究[J]. 临床医学进展, 2026, 16(4): 2851-2861. https://doi.org/10.12677/acm.2026.1641540

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