酶解法提取楠竹叶黄酮的工艺研究

Research on the Extraction Process of Flavonoids from Moso Bamboo Leaves by Enzymatic Hydrolysis

摘要: 竹叶黄酮是一种天然的有机混合物，可以有效地降血糖、血脂，对于心脑血管疾病也有一定的预防作用，而且还可以起到抗氧化作用。本文采用单因素实验法，从酶的种类、料液比、酶解时间和酶的用量四方面，并进行正交实验对楠竹叶总黄酮的提取工艺进行了优化。结果显示，采用纤维素酶，以4 g竹粉为原料，酶用量为0.05 g，酶解时间为1.5 h，料液比为1:16 g/ml时，黄酮得率达最高(1.75%)。本文所采用的酶解法相较于传统的溶剂提取法更为高效环保，为竹叶黄酮提取工艺的研究提供了新的思路，助力竹叶黄酮的开发与利用。

Abstract: Bamboo leaf flavonoids are a natural organic mixture that can effectively lower blood sugar, blood lipids, and also have a certain preventive effect on cardiovascular and cerebrovascular diseases, and can also play an antioxidant role. In this paper, the extraction process of total flavonoids in moso bamboo leaves was optimized from the four aspects of enzyme type, material-liquid ratio, enzyme hydrolysis time and enzyme dosage, and orthogonal experiments were carried out by using the single-factor experimental method. The results showed that when cellulase was used, the enzyme dosage was 0.05 g, the enzymatic hydrolysis time was 1.5 h, and the material-liquid ratio was 1:16 g/ml, the yield of flavonoids reaches its maximum value of 1.75%. Compared with the traditional solvent extraction method, the enzymatic hydrolysis method used in this paper is more efficient and environmentally friendly, which provides a new idea for the research of bamboo leaf flavonoid extraction process, thereby helping the development and utilization of bamboo leaf flavonoids.